关键词: 沙冬青, 脱水素基因AmCIP, 外显子, 内含子, 生物信息学分析

Abstract: According to the full length cDNA of the AmCIP gene we designed primers, amplified its DNA sequence by PCR, and analyzed the gene structure of AmCIP by using bioinformatics methods. Sequence analysis revealed that the DNA sequence of the AmCIP gene is 1 548 bp, containing two exons and one intron, GenBank accession number KU744005. One 78 bp intron is within the ORF of AmCIP gene, and the intron contains enhancer-like element involved in anoxic specific inducibility（GC-motif）and part of a light responsive element（TCCC-motif）. The 3'-UTR contains more cis-acting elements, which involve in light responsiveness, stress responsiveness, hormone responsiveness and so on; the 5'-UTR region contains only a gibberellin responsive element. The cis-acting elements may play a role in transcription and expression regulation of AmCIP gene. Furthermore, the adenine and thymine（A+T）content of AmCIP gene is 64.7% of total bases. The intron of AmCIP gene is 5'-GT......TG-3', not the GT-AG intron standard form. We suggested the mRNA of the AmCIP gene may have unique splice mechanism.

Key words: Ammopiptanthus mongolicus, dehydrin gene AmCIP, exon, intron, bioinformatics analysis