生物技术通报 ›› 2016, Vol. 32 ›› Issue (10): 199-204.doi: 10.13560/j.cnki.biotech.bull.1985.2016.10.024

• 研究报告 • 上一篇    下一篇

疣粒野生稻胚性细胞与叶片抗白叶枯病信号物质和相关抗病基因的表达差异

柳琳1, 彭波2, 蒋春苗3, 4, 程在全4   

  1. 1. 信阳学院,信阳 464000;
    2. 信阳师范学院,信阳 464000;
    3. 云南大学,昆明 650091;
    4. 云南省农业科学院生物技术与种质资源研究所,昆明 650223
  • 收稿日期:2016-03-30 出版日期:2016-10-25 发布日期:2016-10-12
  • 作者简介:柳琳,女,助教,研究方向:生物化学与分子生物学;E-mail:419657124@qq.com
  • 基金资助:
    NSFC项目(31160067),云南省基金重点项目(2012FA005),行业专项项目(201003021),云南青年项目(201301YC00011)

The Expression Differences Between Bacterial Blight Resistance Genes and Signal Materials in Embryonic Cell and Leaf of O.meyeriana Bail

LIU Lin1, PENG Bo2, JIANG Chun-miao3, 4, CHENG Zai-quan4   

  1. 1. Xinyang University,Xinyang 464000;
    2. Xinyang Normal University,Xinyang 464000;
    3. Yunnan University,Kunming 650091;
    4. Biotechnology﹠Genetic Resources Institute,Yunan Academy of Agriculture Sciences,Kunming 650223
  • Received:2016-03-30 Published:2016-10-25 Online:2016-10-12

摘要: 为了研究白叶枯病菌对疣粒野生稻的抗病信号物质和抗病基因的影响,将疣粒野生稻的胚性细胞(即愈伤组织,非特异化)和叶片细胞(已特异化)进行白叶枯病原菌胁迫处理,然后分别检测其抗病信号物质及抗白叶枯病相关基因的表达情况。结果发现,愈伤组织的SA和JA含量无论是未处理还是处理后均高于叶片细胞,这可能与愈伤组织没有特异化、细胞壁较薄致使病原菌易侵入有关,为了防止病原菌的侵害而产生高的表达量,同时说明疣粒野生稻抗白叶枯病能力是在原胚细胞就已具备;ME207基因在疣粒野生稻细胞特异化前后均有表达,而ME281基因则在细胞特异化前是诱导型表达基因、特异化后是组成型表达基因。

关键词: 水稻, 抗病信号物质, 水杨酸(SA), 茉莉酸(JA), 抗白叶枯病基因

Abstract: In order to research the effect of the Oryzae on the resistance signal substance and resistant bacterial blight gene of O.meyeriana Bai,the O.meyeriana embryonic cells(callus,specialized cells)and leaf cells(no-specialized cells)were treated with the Oryzae. Then the content change of resistance signal substance salicylic acid(SA)and jasmonic acid(JA),and the expression of genes related to bacterial blight resistance were detected. The research has shown that the content of SA and JA in callus before and after treatment was higher than that in leaf cells. This may be related to the cell wall of no-specialized callus which are thinner in order to prevent the invasion of pathogenic bacteria.In order to prevent the invasion of pathogenic bacteria,the expression was increased. At the same time,it shows that the ability to resist the Oryzae of the O.meyeriana is in the primary germ cell. The ME207 gene was expressed before and after the specialized differentiation of the O.meyeriana cells. But the ME281 gene is an inducible expression gene before the specialized differentiation of the O.meyeriana cells,which is a constitutive expression gene after the specialized differentiation of the O.meyeriana cells.

Key words: O. meyeriana, resistance signal substance, salicylic acid(SA), jasmonic acid(JA), resistant bacterial blight gene