利用原生质体融合技术构建耐酸絮凝性产乙醇酿酒酵母

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.013

摘要/Abstract

摘要： 为获得具有多种优良性状的高效乙醇生产菌株,以絮凝性酿酒酵母RHZ-1及耐酸耐温酿酒酵母SEB2为亲本,通过紫外诱变获得营养缺陷型标记菌株,并利用原生质体融合技术选育耐酸絮凝性酿酒酵母。通过对融合子的筛选获得一株优秀的耐酸絮凝性酿酒酵母菌株RS-1;该菌株在30℃,pH2.2,15% YPD条件下发酵24 h,乙醇产生量为47.87 g/L,糖消耗速率和基于糖消耗的乙醇收率分别比亲本菌株RHZ-1提高了19.5%和9.8%。在35℃,pH2.2,15% YPD条件下发酵48 h,乙醇产生量为38 g/L,糖消耗速率和乙醇收率分别比亲本菌株RHZ-1提高了46.8%和21.6%。结果表明,获得的菌株可为提高燃料乙醇的生产效率奠定基础。

关键词: 酿酒酵母, 紫外诱变, 原生质体融合, 耐酸性, 乙醇发酵

Abstract: In order to obtain an excellent strain with multi traits for ethanol production,using flocculating strain RHZ-1 and acid-tolerant strain SEB2 as the parents,auxotrophic strains were obtained by UV mutagenesis induction. Saccharomyces cerevisiae strain RS-1 with excellent acid-tolerant and flocculating ability was selected by screening fusants with the technology of protoplast fusion. When fermenting 15% YPD at 30℃ and pH2.2 for 24 h,the ethanol yield was 47.87 g/L,the glucose consumption rate and ethanol yield of strain RS-1 was greatly improved compared to the original strain RHZ-1,increased 19.5% and 9.8%,respectively. When fermenting 15% YPD at 35℃ and pH2.2 for 48 h,the ethanol yield was 38 g/L,the glucose consumption rate and ethanol yield of strain RS-1 increased 46.8% and 21.6%,respectively compared to the original strain RHZ-1. The results suggest that the strain obtained in this study could be a potential microorganism for ethanol industry.

Key words: Saccharomyces cerevisiae, UV mutagenesis, protoplast fusion, acid tolerance, ethanol fermentation

苟敏, 杨白雪, 汤岳琴, 木田建次. 利用原生质体融合技术构建耐酸絮凝性产乙醇酿酒酵母[J]. 生物技术通报, 2016, 32(11): 115-123.

GOU Min, YANG Bai-xue, TANG Yue-qin, Kida Kenji. Construction of Acid-tolerant and Flocculating Saccharomyces cerevisiae Strain for Ethanol Production by Protoplast Fusion[J]. Biotechnology Bulletin, 2016, 32(11): 115-123.

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