黄曲霉核糖体蛋白基因在不同生长时期的表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2017-1049

生物技术通报 ›› 2018, Vol. 34 ›› Issue (4): 194-200.doi: 10.13560/j.cnki.biotech.bull.1985.2017-1049

黄曲霉核糖体蛋白基因在不同生长时期的表达分析

耿龙泼, 王鑫旺, 黄路华, 邓基利, 汪世华, 张峰

福建农林大学生命科学学院福建省病原真菌与真菌毒素重点实验室,福州 350002

收稿日期:2017-12-07 出版日期:2018-04-20 发布日期:2018-05-04
作者简介:耿龙泼,男,硕士研究生,研究方向：病原真菌与真菌毒素;E-mail：glpabc2016@outlook.com
基金资助:
福建省科技计划重点项目（2014Y0047）

Expression Analysis of Ribosomal Protein Genes in Aspergillus flavus

GENG Long-po, WANG Xin-wang, HUANG Lu-hua, DENG Ji-li, WANG Shi-hua, ZHANG Feng

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province,School of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002

Received:2017-12-07 Published:2018-04-20 Online:2018-05-04

摘要/Abstract

摘要： 核糖体是生物合成蛋白质的主要场所。核糖体蛋白是核糖体的重要组分之一。为了探究黄曲霉核糖体蛋白与其生长发育之间的关系,分别收集处于菌丝期、孢子期、菌核期的黄曲霉。在提取了总RNA和反转录获得了cDNA后,利用荧光定量RT-PCR对74个黄曲霉核糖体蛋白基因的表达量进行分析。结果表明,在所有被检测的核糖体蛋白基因中,没有一个基因的表达量在3个时期都是恒定的。与菌丝期相比,在孢子期,有54个核糖体蛋白基因的表达量发生上调,而表达量下调的基因只有2个;在菌核期,有57个基因的表达量上调,而有12个基因的表达量下调。GO功能分析表明,核糖体蛋白基因富集在结构分子活性,结合,细胞,大分子复合物,细胞器,细胞部分,细胞器部分,细胞过程,代谢过程。可见,核糖体蛋白与黄曲霉的生长发育存在紧密联系。

关键词: 黄曲霉, 核糖体蛋白, 黄曲霉毒素, 生长发育, 荧光定量RT-PCR

Abstract: Ribosome is a major organelle of protein synthesis in organism. Ribosomal protein is one of main components of ribosome. In order to explore the relationship between Aspergillus flavus ribosomal protein and its development,A. flavus were collected at mycelial stage,spore stage,and sclerotial stage. After total RNA extraction and the cDNA preparation,the expression levels of 74 genes of A. flavus ribosomal protein were detected by quantitative RT-PCR. The results showed that,none of the tested genes was expressed invariably at all three stages. Compared with mycelial stage,the expression levels of 54 ribosomal protein genes were up-regulated at the spore stage,while 2 genes were down-regulated. At sclerotial stage,the expression levels of 57 genes were up-regulated,while 12 genes were down-regulated. The GO functional analysis showed that the ribosomal protein genes were enriched into structural molecular activity,binding,cell,macromolecular complex,organelle,cell part,organelle part,cell process,and metabolic process. It can be concluded that ribosomal proteins are closely related to the growth and development of A. flavus.

Key words: Aspergillus flavus, ribosomal protein, aflatoxin, growth and development, quantitative RT-PCR

耿龙泼, 王鑫旺, 黄路华, 邓基利, 汪世华, 张峰. 黄曲霉核糖体蛋白基因在不同生长时期的表达分析[J]. 生物技术通报, 2018, 34(4): 194-200.

GENG Long-po, WANG Xin-wang, HUANG Lu-hua, DENG Ji-li, WANG Shi-hua, ZHANG Feng. Expression Analysis of Ribosomal Protein Genes in Aspergillus flavus[J]. Biotechnology Bulletin, 2018, 34(4): 194-200.

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黄曲霉核糖体蛋白基因在不同生长时期的表达分析

Expression Analysis of Ribosomal Protein Genes in Aspergillus flavus

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