水稻OsRhoGAP1基因的序列特征及表达特性分析

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.014

摘要/Abstract

摘要： OsRhoGAP1是本实验室前期从水稻幼穗cDNA文库中分离获得的功能未知基因。通过生物信息学和现代分子生物学技术,系统探讨了水稻OsRhoGAP1的组织表达特性和不同激素处理下的表达情况。生物信息学分析表明,OsRhoGAP1具有植物RhoGAP蛋白典型的结构特征,即CRIB基序和RhoGAP结构域。qRT-PCR检测结果显示该基因的表达具有时空特异性,幼苗期主要在根和叶中表达,幼穗期主要在幼穗和叶中表达,成熟期则主要在根中表达。同时,经ABA、SA、GA和MeJA处理后,OsRhoGAP1基因表达量有明显上调,经IAA和6-BA处理后,OsRhoGAP1基因表达量出现显著下调,表明该基因的表达受到多种激素调控。

关键词: 水稻, OsRhoGAP1基因, 序列分析, 表达分析, 植物激素

Abstract: A function-unknown gene was isolated from a cDNA library of young spike of rice and designated as OsRhoGAP1. Based on the bioinformatics and modern molecular biology,we systematically surveyed its expression patterns at different development stages and under different hormones treatments. Bioinformatics analysis showed that OsRhoGAP1 had typical structural feature of plant RhoGAP protein,including CRIB motif and RhoGAP domain. qRT-PCR analysis revealed that OsRhoGAP1 gene presented specifically spatio-temporal characteristics,i.e.,relative high expression levels in root and leave at the seedling stage,in young panicle and leave at the panicle stage,and in root at the mature stage. Furthermore,the transcription of OsRhoGAP1 was strongly induced by ABA,SA,GA,and MeJA,but suppressed by IAA and 6-BA. It was speculated that the expression of OsRhoGAP1 was regulated by hormones.

Key words: rice, OsRhoGAP1gene, sequence analysis, expression analysis, plant hormones

闫鑫甜,黄俊骏,楼晨,葛慧雯,梁卫红. 水稻OsRhoGAP1基因的序列特征及表达特性分析[J]. 生物技术通报, 2017, 33(5): 94-101.

YAN Xin-tian HUANG Jun-jun LOU Chen GE Hui-wen LIANG Wei-hong. Sequence Characterization and Expression Analysis of OsRhoGAP1 Gene in Rice[J]. Biotechnology Bulletin, 2017, 33(5): 94-101.

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