拟南芥FD与14-3-3/GRF7蛋白的相互作用研究

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.017

生物技术通报 ›› 2017, Vol. 33 ›› Issue (5): 117-122.doi: 10.13560/j.cnki.biotech.bull.1985.2017.05.017

拟南芥FD与14-3-3/GRF7蛋白的相互作用研究

袁敏,王莉,葛伟娜,张岚

华北理工大学生命科学学院基因组学与计算生物学研究中心,唐山 063000

收稿日期:2016-12-05 出版日期:2017-05-25 发布日期:2017-05-19
作者简介:袁敏,女,博士,研究方向：植物生长发育;E-mail：yuanmin308@163.com
基金资助:
国家自然科学基金项目（31401212）,河北省自然科学基金项目（C2014209134）,唐山市科技局项目（14130274a）

Studies on the Interaction Between FD and 14-3-3/GRF7 in Arabidopsis thaliana

YUAN Min WANG Li GE Wei-na ZHANG Lan

Genomics and Computational Biology Research Center,College of Life Science,North China University of Science and Technology,Tangshan 063000

Received:2016-12-05 Published:2017-05-25 Online:2017-05-19

摘要/Abstract

摘要： 为了明确拟南芥FD与14-3-3/GRFs家族成员14-3-3/GRF7之间的互作关系,利用酵母双杂交和双分子荧光互补两种技术分别做了研究。在酵母中,与阴性对照相比,共转化FD-BD与14-3-3/GRF7-AD的酵母菌落在-Leu/-Trp/-His/-Ade四缺培养基上生长良好,表明在酵母中FD与4-3-3/GRF7之间存在直接的相互作用。在烟草中,与阴性对照相比,转化FD-cYFP与14-3-3/GRF7-nYFP,以及FD-nYFP与14-3-3/GRF7-cYFP载体的农杆菌共注射烟草细胞之后均在细胞核内观察到很强的荧光信号,表明在烟草细胞中FD与14-3-3/GRF7之间存在直接的相互作用。两种技术的结果共同证实FD与14-3-3/GRFs家族成员14-3-3/GRF7之间存在直接相互作用。

关键词: FD, 14-3-3/GRF7, 酵母双杂交, 双分子荧光互补

Abstract: In order to prove whether or not there is any interaction between FD and 14-3-3/GRF7 of 14-3-3/GRFs family,both yeast two hybrid and BiFC assays were performed. In yeast,the yeast colonies co-transformed with FD-BD and 14-3-3/GRF7-AD constructs grew well in -Leu/-Trp/-His/-Ade medium comparing with negative controls,indicating that FD directly interacted with 14-3-3/GRF7 in yeast. In tobacco,comparing with negative controls,the obvious YFP fluorescence signals were detected in the tobacco cells in which Agrobacterium co-transformed with FD-cYFP and 14-3-3/GRF7-nYFP,or FD-nYFP and 14-3-3/GRF7-cYFP vectors was injected,revealing that FD also directly interacted with 14-3-3/GRF7 in tobacco. The results by both assays confirm that there is direct interaction between FD and 14-3-3/GRF7 of 14-3-3/GRFs family.

Key words: FD, 14-3-3/GRF7, yeast two hybrid, BiFC

袁敏,王莉,葛伟娜,张岚. 拟南芥FD与14-3-3/GRF7蛋白的相互作用研究[J]. 生物技术通报, 2017, 33(5): 117-122.

YUAN Min WANG Li GE Wei-na ZHANG Lan. Studies on the Interaction Between FD and 14-3-3/GRF7 in Arabidopsis thaliana[J]. Biotechnology Bulletin, 2017, 33(5): 117-122.

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拟南芥FD与14-3-3/GRF7蛋白的相互作用研究

Studies on the Interaction Between FD and 14-3-3/GRF7 in Arabidopsis thaliana

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