关键词: 枸杞, 品种, SSR, 分子身份证

Abstract: Using 16 cultivars as experimental materials,wolfberry molecular ID was constructed by SSR marker,aiming at providing references for identification and protection for wolfberry cultivars. Twenty-four primer pairs with high polymorphism,repeatability and distribution on the 12 chromosomes of wolfberry were screened from 600 pairs of SSR primers,and amplified 16 wolfberry cultivars. A total of 155 alleles were detected,the number of alleles per pair of primers ranged in 3-10,averagely 6.5 alleles per locus. Two hundred and eight genotypes were obtained ranging from 3 to 14 with average of 8.7 genotypes per locus. Polymorphic information content（PIC）ranged from 0.461 to 0.848 with an average of 0.682 per locus. Shannon’s information index ranged from 0.939 to 2.055 with an average of 1.487. Based on the principle of identifying the most cultivars using the minimum pairs of primers,the number of screened alleles,the number of genotypes,and PIC values were great than the average,and the value of Shannon’s information index was great than 1.7. Finally,the combination of primers LBSSR0052 and LBSSR0423 distinguished all tested cultivars. Based on these two pairs of primers,the alleles of all the cultivars were sequenced in descending order,and then the values of each cultivar in these two SSR loci were sequentially combined,thus the molecular ID of 16 wolfberry cultivars were established. The results indicate that SSR marker can be used as an effective technique for the variety identification and molecular ID construction of wolfberry.

Key words: wolfberry, cultivars, SSR, molecular ID