荧光铜纳米簇介导的生物传感器的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0524

摘要/Abstract

摘要： 荧光铜纳米簇（Fluorescent copper nanoclusters,CuNCs）是以脱氧核糖核酸链（Deoxyribonucleic acid,DNA）为模板,以二价铜离子（Cu²⁺）、抗坏血酸等为反应物生成的铜晶体,纳米级大小,其具有荧光性,可以作为生物传感器输出信号的一种方式。荧光铜纳米簇的生成快速、简便、安全,因此近年来涌现出很多关于荧光铜纳米簇原理和应用方面的研究。从支持传感器工作的介导物质以及信号输出方式两方面对荧光铜纳米簇进行分类,详细阐述了每一类别传感器工作的原理,并对比同类型传感器的优缺点,最后对荧光铜纳米簇介导的生物传感器目前存在的不足及今后的发展趋势进行了展望。以便读者对荧光铜纳米簇生物传感器发展历程和方向,对荧光铜纳米簇生物传感器的实用性和多形性有所了解,在未来的研究发展中得到启示,使荧光铜纳米簇成为一种更加实用和便捷的生物传感工具。

关键词: 荧光, 铜, 纳米簇, 生物传感器, 功能核酸

Abstract: Fluorescent copper nanoparticles（CuNCs）are nanometer-sized copper crystals generated by using deoxyribonucleic acid（DNA）as a template and divalent copper ions（Cu²⁺）and ascorbic acid as reactants,and they are fluorescent and can be used to be output signal in biosensors. The generation of CuNCs is fast,simple,and safe. Therefore,many researches on the principles and applications of CuNCs have emerged in recent years. Here CuNCs mediated biosensors are classified in terms of mediators supporting the sensor operation and signal output mode. A detailed description of the principles of various sensors and comparison of the advantages and disadvantages of the biosensors are included,for the purpose of understanding the feasibility and versatility of（CuNCs）mediated biosensors after reading the development and directions of these biosensors. Finally,this review also discusses the shortcomings in applying the CuNCs biosensors and looks into its future development trend. This is aimed to inspire future research and development in this area and thus to develop copper nanoparticles mediated biosensors into more practical and convenient biosensing tool.

Key words: fluorescence, copper, nanocluster, biosensor, functional nucleic acid

刘星雨, 李春晖, 田晶晶, 邵向丽, 罗云波, 许文涛. 荧光铜纳米簇介导的生物传感器的研究进展[J]. 生物技术通报, 2019, 35(1): 170-186.

LIU Xing-yu, LI Chun-hui, TIAN Jing-jing, SHAO Xiang-Li, LUO Yun-bo, XU Wen-tao. Research Progress on Fluorescent Copper Nanoparticles Mediated Biosensors[J]. Biotechnology Bulletin, 2019, 35(1): 170-186.

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