生物技术通报 ›› 2019, Vol. 35 ›› Issue (9): 150-155.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0135

• 研究报告 • 上一篇    下一篇

孔雀石绿降解菌群多样性及高效降解菌的降解特性分析

王亚妮1,2, 宋金龙2, 韩刚2, 穆迎春2, 江旭3, 王金耀4, 阮志勇3, 李乐1,2   

  1. 1. 上海海洋大学,上海 201306;
    2. 中国水产科学研究院,北京 100141;
    3. 中国农业科学院农业资源与农业区划研究所,北京 100081;
    4. 巴彦淖尔市第一中学,巴彦淖尔 015002
  • 收稿日期:2019-02-21 出版日期:2019-09-26 发布日期:2019-09-16
  • 作者简介:王亚妮,女,硕士,研究方向:环境微生物;E-mail:1416872125@qq.com
  • 基金资助:

    中央级公益性科研院所基本科研业务费(2017C004),国家自然科学基金项目(31700006)

Analysis of Diversity of Malachite Green-degrading Microbial Community and Degradation Characteristics of Efficiently-degrading Bacteria

WANG Ya-ni1,2, SONG Jin-long2, HAN Gang2, MU Ying-chun2, JIANG Xu3, WANG Jin-yao4, RUAN Zhi-yong3, LI Le1,2   

  1. 1. Shanghai Ocean University,Shanghai 201306;
    2. Chinese Academy of Fishery Sciences,Beijing 100141;
    3. Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;
    4. Bayannaoer No. 1 Middle School,Bayannaoer 015002
  • Received:2019-02-21 Published:2019-09-26 Online:2019-09-16

摘要:

以三苯甲烷类染料孔雀石绿为唯一碳源,通过连续传代驯化,拟获得能够降解孔雀石绿的富集培养物。运用高通量测序对富集培养物进行了微生物群落区系变化分析,获得了与降解相关的微生物信息,进一步分离纯化到了1株孔雀石绿高效降解菌株。该菌7 d内对50 mg/L孔雀石绿降解效率达91.2%。经初步鉴定,该菌为Pseudomonas菌属菌株,并命名为Pseudomonas veronii JW3-6。对该菌进行了降解条件优化,结果表明菌株最适降解温度为30℃,pH为7,接种浓度为1×107 CFU/mL。

关键词: 孔雀石绿, 高通量测序, 微生物降解, 假单胞菌

Abstract:

Using triphenylmethane dye malachite green as the sole carbon source,the enriched culture degrading malachite green was obtained by continuous passage domestication. High-throughput sequencing was applied to analyze the microbial community change of the enriched culture,and the main microbial information related to degradation was acquired,and further an efficient strain degrading malachite green was isolated and purified. This strain degraded 91.2% of 50 mg/L malachite green within 7 days. The strain was initially identified as a Pseudomonas,and named as Pseudomonas veronii JW3-6.The optimized condition of degradation by this strain were temperature 30℃,pH 7,and inoculation concentration 1×107 CFU/mL.

Key words: malachite green, high-throughput sequencing, microbial degradation, Pseudomonas