核糖体肽生物合成中的典型翻译后修饰研究

doi:10.13560/j.cnki.biotech.bull.1985.2020-0136

摘要/Abstract

摘要：

核糖体肽类天然产物（RiPPs）是由核糖体合成,经由翻译后修饰得到的一大类天然产物,具有广泛的结构和生物活性多样性。综述了核糖体肽的类别及种类众多的翻译后修饰机理,探讨了翻译后修饰对结构多样性的意义,并对生物信息学背景下利用基因组挖掘技术来开发更多RiPPs做了展望。

关键词: 核糖体肽, 后修饰, 生物合成途径, 基因组挖掘

Abstract:

Ribosomally synthesized and post-translationally modified peptides（RiPPs）are a large class of natural products synthesized by ribosome via post-translational modification which feature a wide range of structural and biological diversity. We reviewed the various classes of RiPPs and their post-translationally modification mechanisms,discussed the significance of post-translational modifications,and prospected the development of RiPPs using genome mining under bioinformatics.

Key words: RiPPs, post-modification, biosynthetic pathway, genome mining

汪金秀, 张琪, 丁伟, 陈拓. 核糖体肽生物合成中的典型翻译后修饰研究[J]. 生物技术通报, 2020, 36(10): 215-225.

WANG Jin-xiu, ZHANG Qi, DING Wei, CHEN Tuo. Classic Post-translational Modification in Ribosomally Synthesized and Post-translationally Modified Peptides Biosynthesis[J]. Biotechnology Bulletin, 2020, 36(10): 215-225.

图/表 14

图1 RiPPs类天然产物生物合成逻辑

图2 三种形态特征各异具有抗生素活性的核糖体肽类天然产物

图3 RiPPs种类圆圈大小代表种类数目,颜色深浅代表结构多样性

图4 Nisin作用机制及与lipid II作用图 A：nisin进入细胞质膜;B：nisin通过自身末端的两个氨基酸环与lipid II结合;C、D：通过稳定跨膜取向成孔[15]

图5 RiPPs翻译后修饰过程[16]

图6 I型羊毛硫肽合成酶LanB催化丝氨酸（Ser）和苏氨酸（Thr）脱水[20]

图7 来源于半胱氨酸,丝氨酸和苏氨酸的杂环[22]

图8 Ser、Thr和Trp的烯丙基化后的结构[26]

图9 rSAM依赖的生物学转化 A：rSAM作用机理[31];B：sactipeptide中硫醚键的形成[32,33];C：MIA的形成[34]

图10 I型和II型羊毛硫肽和非羊毛硫肽细菌素的序列比对图[37] 红色：完全保守的氨基酸;蓝色：高度保守的氨基酸

图11 几种环化RiPPs[6]

图12 Microcin C7核苷酸化修饰[42]

图13 PQQ的形成过程[46]

图14 RiPPs基因组挖掘策略流程图

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