生物技术通报 ›› 2019, Vol. 35 ›› Issue (10): 64-70.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0541

• 研究报告 • 上一篇    下一篇

外源茉莉酸对牛皮杜鹃UV-B辐射缓解作用研究

梁东雨, 王禹佳, 苏敖纯, 徐洪伟, 周晓馥   

  1. 吉林师范大学 吉林省植物资源科学与绿色生产重点实验室,四平136000
  • 收稿日期:2019-06-19 出版日期:2019-10-26 发布日期:2019-09-30
  • 作者简介:梁东雨,女,硕士,研究方向:生物学,E-mail:1935431917@qq.com
  • 基金资助:
    国家自然科学基金项目(31070224),吉林省科技发展计划项目(20140101136JC)

Alleviation Effect of Exogenous Jasmonic Acid on Detrimental Effect of Rhododendron chrysanthum Pall Under UV-B Radiation

LIANG Dong-yu, WANG Yu-jia, SU Ao-chun, XU Hong-wei, ZHOU Xiao-fu   

  1. Jilin Provincial Key Laboratory of Plant Resource Science and Green Production,Jilin Normal University,Siping 136000
  • Received:2019-06-19 Published:2019-10-26 Online:2019-09-30

摘要: 为了探究外源茉莉酸在UV-B辐射中可能的作用及缓解作用机制。茉莉酸预处理牛皮杜鹃4 d,PAR和UV-B下辐射48 h,对叶绿素荧光、过氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、NADPH氧化酶和花青素进行测定。结果显示,UV-B辐射使Fm、Fv/Fm、Fv'/Fm'、Fv/Fo、qP、花青素含量以及CAT活性显著降低,qP和rETR快速光响应曲线下降幅度增大,同时显著提高SOD和NADPH氧化酶的活性。JA预处理显著提高Fm、Fv/Fm、Fv'/Fm'、Fv/Fo、qP、花青素含量以及SOD和NADPH氧化酶活性,并且维持MDA含量和CAT活性在对照水平,且使qP、NPQ和rETR快速光响应曲线呈现出最佳状态。这些结果证实了外施茉莉酸能够使牛皮杜鹃具有抗胁迫能力,部分抵消了UV-B辐射对牛皮杜鹃的负面影响的假说。

关键词: 牛皮杜鹃, UV-B辐射, 茉莉酸, 叶绿素荧光, 抗氧化酶

Abstract: The objective of this work is to explore the feasible role of jasmonic acid in UV-B radiation and the mechanism of its mitigation. Jasmonic acid pretreated Rhododendron chrysanthum for 4 d,then PAR and UV-B radiation was conducted for 48 h,and chlorophyll fluorescence parameters,superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA),NADPH oxidase and anthocyanin were measured.UV-B radiation induced a significant reduction of Fm,Fv/Fm,Fv'/Fm',Fv/Fo,qP,anthocyanin content,and CAT activity,and increased the decreased amplitude of qP and rETR rapid photoresponse curves,while significantly increased the activity of SOD and NADPH oxidase. JA pretreatment obviously mitigated the detrimental effect of UV-B on R. chrysanthum by increasing Fm,Fv/Fm,Fv'/Fm',Fv/Fo,qP,anthocyanin content,and SOD and NADPH oxidase activities,and maintained MDA content and CAT activity at the control level,and the qP,NPQ and rETR fast light response curves presented the optimal state. In conclusion,these results confirm that the hypothesis of the external application of jasmonic acid can make R. chrysanthum resistant to stress,partially offset the negative effect of UV-B radiation on R. chrysanthum.

Key words: Rhododendron chrysanthum Pall., UV-B radiation, jasmonic acid, chlorophyll fluorescence, antioxidant enzyme.