ANGPTL4在肠道微生物影响动物脂肪代谢中的作用

doi:10.13560/j.cnki.biotech.bull.1985.2020-0028

摘要/Abstract

摘要： 血管生成素样蛋白4（Angiopoietin-like protein 4,ANGPTL4）是一种与动物脂肪代谢密切相关的蛋白,在动物体多组织广泛表达,是沟通肠道菌群和宿主脂肪代谢的重要媒介之一。综述了肠道微生物与宿主脂肪沉积的关系、肠道微生物对宿主ANGPTL4的影响、ANGPTL4在动物脂肪代谢中的作用以及肠道微生物-ANGPTL4-动物脂肪代谢关系,讨论了肠道微生物通过ANGPTL4调节动物脂肪代谢研究的意义及存在的问题,为将来更好解释益生菌和酸化剂等饲料添加剂影响畜禽脂肪沉积、促进生产性能的现象提供依据。

关键词: 血管生成素样蛋白4, 肠道微生物, 脂肪代谢

Abstract: Angiopoietin-like protein 4（ANGPTL4）is a protein that is closely related to animal lipid metabolism and widely expressed in many tissues of animals. It is recognized as an important medium to communicate intestinal microbiota and host lipid metabolism. This article summarizes the relationship between gut microbes and host fat deposition,the effect of gut microbes on host ANGPTL4,the role of ANGPTL4 on animal lipid metabolism,and the relationship among gut microbes,ANGPTL4 and animal lipid metabolism. Also this article discusses the significance and existing issues in studying intestinal microbiota affecting lipid metabolism of animals via ANGPTL4,aiming at providing a better explanation for the effects of feed additives such as probiotics and acidifiers on fat deposition and production performance of livestock and poultry.

Key words: angiopoietin-like protein 4, intestinal microbiota, lipid metabolism

赵旭, 徐群, 侯彦茹, 李明宇, 张雅宁, 汪海. ANGPTL4在肠道微生物影响动物脂肪代谢中的作用[J]. 生物技术通报, 2020, 36(6): 230-235.

ZHAO Xu, XU Qun, HOU Yan-ru, LI Ming-yu, ZHANG Ya-ning, WANG Hai. Effects of ANGPTL4 on Intestinal Microbiota Affecting Lipid Metabolism of Animals[J]. Biotechnology Bulletin, 2020, 36(6): 230-235.

参考文献

[1] Ley RE, Bäckhed F, Turnbaugh P, et al.Obesity alters gut microbial ecology[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(31):11070-11075.
[2] Ley RE, Turnbaugh PJ, Klein S, et al.Microbial ecology:human gut microbes associated with obesity[J]. Nature, 2006, 444(7122):1022-1023.
[3] Turnbaugh PJ, Ley RE, Mahowald MA, et al.An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature, 2006, 444(7122):1027-1031.
[4] Guo X, Xia X, Tang R, et al.Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs[J]. Letters in Applied Microbiology, 2008, 47(5):367-373.
[5] Guo X, Xia X, Tang R, et al.Real-time PCR quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs[J]. Anaerobe, 2008, 14(4):224-228.
[6] Angelakis E, Raoult D.The increase of Lactobacillus species in the gut flora of newborn broiler chicks and ducks is associated with weight gain[J]. PLoS One, 2010, 5(5):e10463.
[7] Xu A, Lam MC, Chan KW, et al.Angiopoietin-like protein 4 decreases blood glucose and improves glucose tolerance but induces hyperlipidemia and hepatic steatosis in mice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(17):6086-6091.
[8] Alex S, Lange K, Amolo T, et al.Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor γ[J]. Molecular and Cellular Biology, 2013, 33(7):1303-1316.
[9] Bäckhed F, Ding H, Wang T, et al.The gut microbiota as an environmental factor that regulates fat storage[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(44):15718-15723.
[10] 严鸿林. 肠道微生物及其与营养互作对猪骨骼肌表型及代谢的调控[D]. 成都:四川农业大学, 2018.
[11] Zhao X, Guo Y, Guo S, et al.Effects of Clostridium butyricum and Enterococcus faecium on growth performance, lipid metabolism and cecal microbiota of broiler chickens[J]. Applied Microbiology and Biotechnology, 2013, 97(14):6477-6488.
[12] Zhao X, Ding X, Yang Z, et al.Effects of Clostridium butyricum on growth performance, lipid metabolism and the caecal microecological environment of broilers[J]. European Poultry Science. DOI:10. 1399/eps. 2017, 187.
[13] Yang X, Zhang B, Guo Y, et al.Effects of dietary lipids and Clostridium butyricum on fat deposition and meat quality of broiler chickens[J]. Poultry Science, 2010, 89(2):254-260.
[14] Zhang B, Yang X, Guo Y, et al.Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens[J]. Animal, 2011, 5(12):1909-1915.
[15] Grootaert C, Van de Wiele T, Van Roosbroeck I, et al. Bacterial monocultures, propionate, butyrate and H₂O₂ modulate the expression, secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines[J]. Environmental Microbiology, 2011, 13(7):1778-1789.
[16] 张丽萍. 不同品种猪肠道微生物与体脂、ANGPTL4基因关系的研究[D]. 成都:四川农业大学, 2007.
[17] Aronsson L, Huang Y, Parini P, et al.Decreased fat storage by Lactobacillus paracasei is associated with increased levels of angiopoietin-like 4 protein(ANGPTL4)[J]. PLoS One, 2010, 5(9):e13087.
[18] Zhao X, Ding X, Yang Z, et al.Effects of Clostridium butyricum on breast muscle lipid metabolism of broilers[J]. Italian Journal of Animal Science, 2018, 17(4):1010-1020.
[19] Zhao X, Guo Y, Liu H, et al.Clostridium butyricum reduce lipogenesis through bacterial wall components and butyrate[J]. Applied Microbiology and Biotechnology, 2014, 98(17):7549-7557.
[20] Grootaert C, Van de Wiele T, Verstraete W, et al. Angiopoietin-like protein 4:health effects, modulating agents and structure-function relationships[J]. Expert Review of Proteomics, 2012, 9(2):181-199.
[21] Lu B, Moser A, Shigenaga JK, et al.The acute phase response stimulates the expression of angiopoietin like protein 4[J]. Biochemical and Biophysical Research Communications, 2010, 391(4):1737-1741.
[22] 郭亮. ANGPTL4在LPS诱导的急性肺损伤中的作用及机制研究[D]. 重庆:第三军医大学, 2015.
[23] Korecka A, de Wouters T, Cultrone A, et al. ANGPTL4 expression induced by butyrate and rosiglitazone in human intestinal epithelial cells utilizes independent pathways[J]. American Journal of Physiology Gastrointestinal and Liver Physiology, 2013, 304(11):G1025-1037.
[24] Liu L, Zhuang X, Jiang M, et al.ANGPTL4 mediates the protective role of PPARγ activators in the pathogenesis of preeclampsia[J]. Cell Death & Disease, 2017, 8(9):e3054.
[25] 段志轲. 2型糖尿病及其微血管并发症患者血清血管生成素样蛋白4水平及影响因素分析[D]. 福州:福建医科大学, 2017.
[26] Greiner T, Bäckhed F.Effects of the gut microbiota on obesity and glucose homeostasis[J]. Trends in Endocrinology and Metabolism, 2011, 22(4):117-123.
[27] Yoshida K, Shimizugawa T, Ono M, et al.Angiopoietin-like protein 4 is a potent hyperlipidemia-inducing factor in mice and inhibitor of lipoprotein lipase[J]. Journal of Lipid Research, 2002, 43(11):1770-1772.
[28] Mandard S, Zandbergen F, van Straten E, et al. The fasting-induced adipose factor/angiopoietin-like protein 4 is physically associated with lipoproteins and governs plasma lipid levels and adiposity[J]. The Journal of Biological Chemistry, 2006, 281(2):934-944.
[29] Bäckhed F, Manchester J K, Semenkovich C F, et al.Mechanisms underlying the resistance to diet-induced obesity in germ-free mice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(3):979-984.
[30] Ge H, Yang G, Yu X, et al.Oligomerization state-dependent hyperlipidemic effect of angiopoietin-like protein 4[J]. Journal of Lipid Research, 2004, 45(11):2071-2079.
[31] Köster A, Chao YB, Mosior M, et al.Transgenic angiopoietin-like(angptl)4 overexpression and targeted disruption of angptl4 and angptl3:Regulation of triglyceride metabolism[J]. Endocrinology, 2005, 146(11):4943-4950.
[32] Lichtenstein L, Berbée JF, van Dijk SJ, et al. Angptl4 upregulates cholesterol synthesis in liver via inhibition of LPL- and HL-dependent hepatic cholesterol uptake[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2007, 27(11):2420-2427.
[33] Singh AK, Aryal B, Chaube B, et al.Brown adipose tissue derived ANGPTL4 controls glucose and lipid metabolism and regulates thermogenesis[J]. Molecular Metabolism,2018,11:59-69.
[34] 邹思湘. 动物生物化学[M]. 第5版. 北京:中国农业出版社,2013.