生物技术通报 ›› 2020, Vol. 36 ›› Issue (9): 211-217.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0090

• 根际微生物专题(专题主编:张瑞福 研究员) • 上一篇    下一篇

羊肚菌菌丝体对土壤酶活性的影响

魏晶晶, 张浩然, 王志鸽, 王慧春   

  1. 青海师范大学生命科学学院 青海省青藏高原药用动植物资源重点实验室,西宁 810008
  • 收稿日期:2020-01-29 出版日期:2020-09-26 发布日期:2020-09-30
  • 作者简介:魏晶晶,女,硕士研究生,研究方向:化学生态学;E-mail:1213415397@qq.com
  • 基金资助:
    青海省科学基金(2019-ZJ-913),青海省创新平台建设专项(2017-ZJ-Y20)

Effect of Morchella Mycelium on Soil Enzymatic Activity

WEI Jing-jing, ZHANG Hao-ran, WANG Zhi-ge, WANG Hui-chun   

  1. Key Laboratory of Medicinal Plant and Animal,College of Life Sciences of Qinghai Normal University,Xining 810008
  • Received:2020-01-29 Published:2020-09-26 Online:2020-09-30

摘要: 探究羊肚菌菌丝体对土壤酶活性的影响。通过单因素和正交试验,采用3,5-二硝基水杨酸比色法测定羊肚菌菌丝体对土样中蔗糖酶和淀粉酶活性的影响。结果显示,菌土影响土壤蔗糖酶活性最佳条件为温度28℃,时间3 d,菌丝体添加量为1∶5,土壤蔗糖酶活性增加为对照组的12倍;土壤淀粉酶活性的最佳条件为时间7 d,菌丝体添加量1∶5,温度28℃,土壤淀粉酶活性增加为对照组的18倍。在短期内羊肚菌菌丝体能提高土壤中蔗糖酶和淀粉酶活性,对改良土壤具有重要意义。

关键词: 羊肚菌菌丝体, 蔗糖酶, 淀粉酶

Abstract: This work aims to study on the effect of Morchella mycelium on soil enzymatic activity. The sucrase and amylase activities of soil samples were determined by 3,5-dinitrosalicylic acid colorimetric method with single factor and orthogonal experimental design. The optimal condition of the soil sucrase activity was that the temperature was 28℃,the time was 3 d,the amount of added mycelium was 1∶5,and the soil sucrase activity increased 12 times compared with the control group. The optimal condition of soil amylase activity was as such:the amount of added mycelium was 1∶5,the temperature was 28℃,the time was 7 d,and the soil amylase activity increased 18 times compared with the control group. Morchella mycelium can improve the sucrase and amylase activities in the soil in a short period of time,which is of significance for improving soil.

Key words: Morchella mycelium, sucrase, amylase