一种具有琼胶酶活性的海洋细菌淀粉酶的表达和分析

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.018

生物技术通报 ›› 2017, Vol. 33 ›› Issue (2): 125-130.doi: 10.13560/j.cnki.biotech.bull.1985.2017.02.018

一种具有琼胶酶活性的海洋细菌淀粉酶的表达和分析

林伯坤^1,2, 宋燕², 陆国永², 赵敏², 钟名其², 胡忠²

1. 广东医科大学公共卫生学院东莞市环境医学重点实验室,东莞 523808;
2. 汕头大学广东省海洋生物技术重点实验室,汕头 515063

收稿日期:2016-05-19 出版日期:2017-02-26 发布日期:2017-02-08
作者简介:林伯坤,男,博士,研究方向：生物技术、卫生检验学;E-mail：albertbklin@foxmail.com
基金资助:
国家自然科学基金项目（31200077,41476150）,广东省扬帆计划（4YF16003G）,广东省海洋生物技术重点实验室开放基金项目（GPKLMB201504）

Expression and Analysis of an Amylase with Agarase Activity from a Marine Bacterium

LIN Bo-kun^1,2, SONG Yan², LU Guo-yong², ZHAO Min², ZHONG Ming-qi², HU Zhong²

1. Dongguan Key Laboratory of Environmental Medicine,School of Public Health,Guangdong Medical University,Dongguan 523808;
2. Guangdong Provincial Key Laboratory of Marine Biotechnology,Shantou University,Shantou 515063

Received:2016-05-19 Published:2017-02-26 Online:2017-02-08

摘要/Abstract

摘要： 旨在验证海洋新种细菌ZC1中可能存在的具有琼胶酶活性的淀粉酶。对菌株ZC1中的淀粉酶编码基因amy440进行基因克隆和重组原核表达,并用活性电泳法验证重组蛋白的淀粉酶和琼胶酶活性。结果显示,基因amy440的重组蛋白在淀粉酶和琼胶酶活性电泳上均出现活性显色条带。菌株ZC1中基因amy440的编码蛋白是一种具有琼胶酶活性的淀粉酶。

关键词: 琼胶酶, 淀粉酶, 多功能酶, 基因克隆, 重组表达

Abstract: Our aim is to confirm that there is amylase with agarase activity in the novel marine bacterial strain ZC1. The amylase gene amy440 of strain ZC1 was cloned and expressed in recombinant prokaryotic vector and the amylase and agarase activity of the recombinant protein was determined using the method of activity staining electrophoresis. Results indicated that the recombinant protein of gene amy440 showed amylase and agarase activity in the activity staining electrophoresis. Conclusively,the encoded protein of gene amy440 of strain ZC1 is an amylase with agarase activity.

Key words: agarase, amylase, multifunctional enzyme, gene cloning, recombinant expression

林伯坤, 宋燕, 陆国永, 赵敏, 钟名其, 胡忠. 一种具有琼胶酶活性的海洋细菌淀粉酶的表达和分析[J]. 生物技术通报, 2017, 33(2): 125-130.

LIN Bo-kun, SONG Yan, LU Guo-yong, ZHAO Min, ZHONG Ming-qi, HU Zhong. Expression and Analysis of an Amylase with Agarase Activity from a Marine Bacterium[J]. Biotechnology Bulletin, 2017, 33(2): 125-130.

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一种具有琼胶酶活性的海洋细菌淀粉酶的表达和分析

Expression and Analysis of an Amylase with Agarase Activity from a Marine Bacterium

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