白蚁肠道木质素降解菌分离鉴定及其降解特性

doi:10.13560/j.cnki.biotech.bull.1985.2020-0128

生物技术通报 ›› 2020, Vol. 36 ›› Issue (8): 61-68.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0128

白蚁肠道木质素降解菌分离鉴定及其降解特性

李锋^1,2, 黄庶识²

1.黔南民族师范学院化学化工学院,都匀 558000;
2.广西科学院广西海洋天然产物与组合生物合成重点实验室,南宁 530007

收稿日期:2020-02-12 出版日期:2020-08-26 发布日期:2020-08-27
作者简介:李锋,男,博士,副教授,研究方向：环境微生物学;E-mail：huagonglflf@163.com
基金资助:
国家自然科学基金项目（31560017）,广西自然科学基金重点项目（2014GXNSFDA118012）,黔南民族师范学院引进高层次人才研究专项（qnsy2019）

Isolation and Identification of Lignin-degrading Bacteria from the Gut of Termite and Their Degradation Characteristics

LI Feng^1,2, HUANG Shu-shi²

1. School of Chemistry and Chemical Engineering,Qiannan Normal College for Nationalities,Duyun 558000;
2. Guangxi Academy of Sciences,Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry,Nanning 530007

Received:2020-02-12 Published:2020-08-26 Online:2020-08-27

摘要/Abstract

摘要： 以白蚁肠道为菌株来源,富集分离木质素降解细菌,并进一步解析木质素降解过程。从白蚁肠道中筛选木质素降解菌,对其进行16S rRNA基因序列鉴定,通过FTIR和TGA等手段解析了该菌对木质素的降解特性。结果显示,菌株MP-132经鉴定为解鸟氨酸拉乌尔菌,以碱木质素为唯一碳源时,培养7 d后,木质素降解率可达53.2%,漆酶和木质过氧化物酶活力最大分别为32 U/L和105.6 U/L。该菌能够使苯胺蓝和天青B脱色。此外,FTIR和TGA分析结果表明细菌生物处理改变了木质素结构。较为系统揭示了菌株MP-132的木质素降解特性,为木质素资源化利用方面提供优良菌株资源。

关键词: 木质素, 拉乌尔菌, 白蚁, 降解

Abstract: The objectives of this work are to enrich and isolate lignin-degrading bacteria from the gut of termite,and to study their degradation characteristics. A lignin-degrading bacterium was first isolated from the gut of a termite species,Microtermes pakistanicus,and then identified by 16S rRNA gene sequence analysis for their phylogeny property. Further,with a variety of evaluation methods,such as FTIR and TGA,the degradation characteristics of lignin for the strain were analyzed. As results,a strain MP-132 was identified as Raoultella ornithinolytica. The degradation efficiency was up to 53.2% after 7 d culture when alkaline lignin was used as sole carbon source. Laccase and lignin peroxidase peroxidase reached maximum activities 32 U/L and 105.6 U/L,respectively. Moreover,strain MP-13 led the azure B and aniline blue decolorized. The results from the FTIR and TGA analysis showed that strain MP-132 uniquely presented an attractive capability to deconstruct lignin components. In conclusion,this work systematically reveals the degradation characteristics of strain MP-132,which may provide fine germplasm resources for the biodegradation of lignin.

Key words: lignin, Raoultella, termite, degradation

李锋, 黄庶识. 白蚁肠道木质素降解菌分离鉴定及其降解特性[J]. 生物技术通报, 2020, 36(8): 61-68.

LI Feng, HUANG Shu-shi. Isolation and Identification of Lignin-degrading Bacteria from the Gut of Termite and Their Degradation Characteristics[J]. Biotechnology Bulletin, 2020, 36(8): 61-68.

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白蚁肠道木质素降解菌分离鉴定及其降解特性

Isolation and Identification of Lignin-degrading Bacteria from the Gut of Termite and Their Degradation Characteristics

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