嗜热纤维素降解菌研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2022-1240

生物技术通报 ›› 2023, Vol. 39 ›› Issue (6): 73-87.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1240

嗜热纤维素降解菌研究进展

张晶¹^,²^,³(), 张浩睿⁴^,⁵, 曹云¹^,²^,³, 黄红英¹^,²^,³(), 曲萍¹^,²^,³, 张志萍⁴^,⁵()

1.江苏省农业科学院农业资源与环境研究所，南京 210014
2.农业农村部长江下游平原农业环境重点实验室，南京 210014
3.江苏省有机固体废弃物资源化协同创新中心，南京 210095
4.河南农业大学机电工程学院，郑州 450002
5.农业农村部可再生能源新材料与装备重点实验室，郑州 450002

收稿日期:2022-10-09 出版日期:2023-06-26 发布日期:2023-07-07
通讯作者: 张志萍，女，博士，副教授，研究方向：生物质能源开发利用；E-mail: zhangzhipingHAU@henau.edu.cn；
黄红英，男，硕士，研究员，研究方向：农业废弃物资源化利用；E-mail: sfmicrolab@163.com
作者简介:张晶，女，博士，助理研究员，研究方向：农业废弃物资源化利用；E-mail: zhangjing@jaas.ac.cn
基金资助:
国家自然科学基金面上项目(52276183);国家自然科学基金面上项目(CX(20)3076);国家自然科学基金面上项目(ZX(21)1221);2022年河南农业大学研究生教育改革与质量提升工程项目(NDYJSGC2022-7)

Research Progress in Thermophilic Microorganisms for Cellulose Degradation

ZHANG Jing¹^,²^,³(), ZHANG Hao-rui⁴^,⁵, CAO Yun¹^,²^,³, HUANG Hong-ying¹^,²^,³(), QU Ping¹^,²^,³, ZHANG Zhi-ping⁴^,⁵()

1. Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
2. Key Laboratory of Agricultural Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014
3. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095
4. College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002
5. Key Laboratory of New Materials and Facilities for Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Zhengzhou 450002

Received:2022-10-09 Published:2023-06-26 Online:2023-07-07

摘要/Abstract

摘要：

纤维素是地球上含量丰富的可再生资源，对其进行充分利用可有效缓解日益严峻的能源和环境问题。纤维素酶是纤维素生物转化的关键，其催化效果决定了纤维素能源化利用的商业价值。鉴于常规的中温酶很难适应工业化生产中的极端条件，来源于嗜热微生物的纤维素酶引起了科研人员关注。结合国内外研究现状，综述了嗜热纤维素降解菌的种类、纤维素酶类型以及纤维素降解方式，讨论了嗜热纤维素酶在工业生产中的作用及应用现状。最后提出了嗜热纤维素酶大规模应用的技术瓶颈和今后的研究重点，展望了嗜热纤维素酶的商业化前景。

关键词: 嗜热微生物, 纤维素酶, 降解方式

Abstract:

Cellulosic biomass is a geographically abundant renewable resource, showing enormous potential to contribute to alleviate the escalating energy and environmental problems. Cellulase is the key to bioconversion of cellulose, and its catalytic effect determines the commercial potential of using cellulose for bioenergy production. In view of the fact that it is difficult for conventional mesophilic enzymes are to adapt to harsh conditions of industrial processing, cellulase from thermophilic microorganisms has attracted the attention of researchers. In this paper, recent progress in thermophilic cellulolytic microorganisms, cellulase types and cellulose degradation strategies was reviewed. Further, the role and application status of thermophilic cellulase in industrial production were discussed. Finally, the technical bottleneck and research emphases of large-scale application of thermophilic cellulose were presented, and the commercial prospect of thermophilic cellulose was prospected.

Key words: thermophilic microorganism, cellulase, degradation paradigm

张晶, 张浩睿, 曹云, 黄红英, 曲萍, 张志萍. 嗜热纤维素降解菌研究进展[J]. 生物技术通报, 2023, 39(6): 73-87.

ZHANG Jing, ZHANG Hao-rui, CAO Yun, HUANG Hong-ying, QU Ping, ZHANG Zhi-ping. Research Progress in Thermophilic Microorganisms for Cellulose Degradation[J]. Biotechnology Bulletin, 2023, 39(6): 73-87.

图/表 9

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嗜热纤维素降解菌研究进展

Research Progress in Thermophilic Microorganisms for Cellulose Degradation

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菌种名称（现行命名法） Species（present nomenclature）	其他名称 Other names	培养温度 Culture temperature/℃	纤维素酶类型 Type of cellulase	参考文献 References
Chaetomium thermophilum	Chaetomium thermophile	50	内切葡聚糖酶、外切葡聚糖酶、 β-葡萄糖苷酶	[10]
Melanocarpus albomyces	Myriococcum albomyces, Thielavia albomyces	45	内切葡聚糖酶、纤维二糖水解酶	[11]
Malbranchea cinnamomea	Trichothecium cinnamomeum, Thermoidium sulfureum, Malbranchea pulchella var.sulfurea	45	内切葡聚糖酶、纤维二糖水解酶、β-葡萄糖苷酶	[12]
Mycothermus thermophilus	Torula thermophila Scytalidium thermophilum	45	内切葡聚糖酶、纤维二糖水解酶、β-葡萄糖苷酶	[13]
Rasamsonia emersonii	Talaromyces emersonii	45	内切葡聚糖酶、外切葡聚糖酶、 β-葡萄糖苷酶	[14]
Thermoascus aurantiacus		50	内切葡聚糖酶、纤维二糖水解酶、β-葡萄糖苷酶	[15]
Thermomyces lanuginosus	Sepedonium lanuginosum Monotospora lanuginosa Humicola lanuginosa	50	内切葡聚糖酶、外切葡聚糖酶、 β-葡萄糖苷酶	[16-17]

菌种名称Species	培养温度Culture temperature/℃	纤维素酶类型Type of cellulase	参考文献Reference
Clostridium thermocellum	60	内切葡聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶	[29]
Caldicellulosiruptor saccharolyticus	70	内切葡聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶	[30]
Geobacillus thermoleovorans	60	内切葡聚糖酶	[31]
Acidothermus cellulolyticus	55	内切葡聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶	[32]
Alicyclobacillus acidocaldarius	60	内切葡聚糖酶	[33]
Caldibacillus cellulovorans	70	内切葡聚糖酶	[34]

菌种名称Species	培养温度Culture temperature /℃	纤维素酶类型Type of cellulase	参考文献References
Thermomonospora curvata	52	内切葡聚糖酶、β-葡萄糖苷酶	[48-49]
Thermobispora bispora	58	内切葡聚糖酶、纤维二糖水解酶、β-葡萄糖苷酶	[50]
Streptomyces thermoalkaliphilus	50	内切葡聚糖酶	[51]
Thermobifida fusca	50	内切葡聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶	[52]
Thermobifida halotolerans	45	内切葡聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶	[53-54]
Thermoactinospora rubra	50	内切葡聚糖酶、β-葡萄糖苷酶	[55]

来源菌株Microorganism	酶的名称Enzyme	酶的结构Modules	酶的活性Activity	参考文献Reference
Rasamsonia emersonii	CBHII	CBM1, GH6	纤维二糖水解酶	[63]
Rasamsonia emersonii	CBHIB	GH7	纤维二糖水解酶	[64]
Chaetomium thermophilum	CBH1	GH7, CBM1	纤维二糖水解酶	[65]
	CBH2	CBM1, GH6	纤维二糖水解酶	[66]
	CBH3	GH7	纤维二糖水解酶	[67]
Thermoascus aurantiacus	CBH1	GH7	纤维二糖水解酶	[68]
Thermoascus aurantiacus	EGI	GH5	内切-β-1,4-葡聚糖酶	[69]
Thermobifida fusca	Cel5A	CBM2, GH5	内切-β-1,4-葡聚糖酶	[70]
	Cel6A	GH6, CBM2	内切-β-1,4-葡聚糖酶	[70]
	Cel9A	GH9, CBM3, CBM2	内切-β-1,4-葡聚糖酶	[70]
	Cel48A	CBM2, GH48	外切-β-1,4-葡聚糖酶	[70]
Thermobifida halotolerans	Cel5A	CBM2, GH5	内切-β-1,4-葡聚糖酶	[53]
Thermobispora bispora	BglB	GH1	β-葡萄糖苷酶	[71]
Acidothermus cellulolyticus	Cel5A	GH5, CBM2	内切-β-1,4-葡聚糖酶	[42]
Alicyclobacillus acidocaldarius	CelA	GH9	内切-β-1,4-葡聚糖酶	[72]
Rhodothermus marinus	CelA	GH12	内切-β-1,4-葡聚糖酶	[73]
Caldicellulosiruptor bescii	CelA	GH9^a, CBM3, CBM3, CBM3, GH48^b	^a内切-β-1,4-葡聚糖酶，^b纤维二糖水解酶	[74]
Geobacillus sp.	CelA	GH5	内切-β-1,4-葡聚糖酶	[75]

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