固定化小球藻产氧及光合速率的研究

doi:10.13560/j.cnki.biotech.bull.1985.2020-0860

摘要/Abstract

摘要：

通过对固定化小球藻产氧及光合速率的研究,进一步解决水产养殖水体溶解氧不足的问题,为生物增氧技术投入使用奠定基础。固定化具有较高的生物量及生物活性,在研究中被广泛使用。运用固定化技术对固定化小球藻产氧及光合速率进行了研究,结果表明,固定化小球藻具有较好的生物量及生物活性,海藻酸钠+羧甲基纤维素钠+硅藻土作为固定化基质有利于小球藻的放氧,其最高放氧量介于第3-4天,对于1 L水体增氧的最适固定化小球藻体积为150 mL,固定化小球藻的光合速率明显高于游离态小球藻。固定化技术增加了小球藻生物量和生物活性,具有较好的生物增氧特性,是较好的生物增氧材料。

关键词: 固定化, 小球藻, 溶解氧

Abstract:

To further solve the problem of insufficient dissolved oxygen in aquaculture and lay the foundation for the application of biological oxygenation technology,the oxygen release and photosynthetic rate of immobilized chlorella were studied. Because of its high biomass and biological activity,immobilization had been widely used in research. The oxygen production and photosynthetic rate of immobilized chlorella were studied by immobilization technique. The results showed that the immobilized chlorella biomass was higher than the free chlorella’s,composite immobilized material（sodium carboxymethyl cellulose +sodium alginate + diatomite）was conducive to the release of oxygen on chlorella and its top release between 3-4 d,the optimal immobilized chlorella volume was 150 mL providing oxygen for 1 L water,and net photosynthetic rate of immobilized chlorella was obviously higher than that of free chlorella. The immobilization increases the biomass and biological activity of chlorella,and has better biological oxygenation characteristics,it is a fine biological oxygenation material.

Key words: immobilization, chlorella, dissolved oxygen

芦尚德, 刘婧婧, 冯一平, 赵鹏, 徐仰仓. 固定化小球藻产氧及光合速率的研究[J]. 生物技术通报, 2021, 37(3): 92-98.

LU Shang-de, LIU Jing-jing, FENG Yi-ping, ZHAO Peng, XU Yang-cang. Study on Oxygen Release and Photosynthetic Rate of Immobilized Chlorella[J]. Biotechnology Bulletin, 2021, 37(3): 92-98.

图/表 9

图1 固定化小球藻和游离态小球藻生物量的比较

图2 固定化小球藻和游离小球藻的氧气释放量对比

图3 加硅藻土固定化小球藻和单一固定化小球藻的氧气释放量对比

图4 加羧甲基纤维素钠固定化小球藻和单一固定化小球藻的氧气释放量对比

图5 加羧甲基纤维素钠固定化小球藻和游离态小球藻的氧气释放量对比

图6 加羧甲基纤维素钠和硅藻土固定化小球藻和加羧甲基纤维素钠固定化小球藻的氧气释放量对比

图7 复合固定化小球藻和游离态小球藻的氧气释放量对比

图8 固定化小球藻数量对放氧的影响

图9 固定化小球藻和游离态小球藻净光合速率的比较

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