粗甘油发酵生产1, 3-丙二醇的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2021-0603

摘要/Abstract

摘要：

粗甘油是生物柴油工业生产中的主要副产物，通过微生物发酵直接将其转化成高值化学品1, 3-丙二醇，是其绿色高值化利用的有效途径。对微生物代谢甘油产1, 3-丙二醇途径及关键酶、粗甘油杂质对微生物发酵影响、不同微生物直接发酵粗甘油生产1, 3-丙二醇以及混菌发酵粗甘油提高1, 3-丙二醇产率等方面的最新研究进展进行了综述，旨在为直接发酵粗甘油生产1, 3-丙二醇的工程菌株开发及其工业化应用提供参考。

关键词: 粗甘油, 微生物发酵, 1, 3-丙二醇, 杂质

Abstract:

Crude glycerol is the main by-product in biodiesel industrial. Direct bioconversion of crude glycerol into 1, 3-propanediol by microbial fermentation is promising for the value-added utilization of crude glycerol. This review outlines glycerol-based 1, 3-propanediol synthetic pathways and key enzymes，the effect of crude glycerol on microbial fermentation，the diverse 1, 3-propanediol-producing microbes involved in crude glycerol fermentation as well as improvement of crude glycerol conversion rate by microbial consortium. This review aims to provide insights for engineering 1, 3-propanediol-producing strain and improving bioconversion of crude glycerol.

Key words: crude glycerol, microbial fermentation, 1, 3-propanediol, impurity

蒋欢, 马江山, 曾柏全, 张良波, 李培旺. 粗甘油发酵生产1, 3-丙二醇的研究进展[J]. 生物技术通报, 2022, 38(10): 45-53.

JIANG Huan, MA Jiang-shan, ZENG Bai-quan, ZHANG Liang-bo, LI Pei-wang. Research Progress in 1, 3-Propanediol Production by Fermenting Crude Glycerol[J]. Biotechnology Bulletin, 2022, 38(10): 45-53.

图/表 2

参考文献 64

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菌株或菌群 Strain or microbial consortium	底物 Substrate	产率 Yield	产量Concent-ration/（g·L^-1）	生产强度Produc- tivity/（g·L^-1·h^-1）	发酵方式 Fermentation	文献 Reference
K. pneumoniae ATCC 8724	粗甘油	0.65 mol/mol	20	-	补料发酵	[48]
K. pneumoniae 2e	粗甘油	0.64 mol/mol	10.28	-	分批发酵	[46]
K. pneumoniae ATCC 200721 ∆ldhA	粗甘油	-	81.1	3.38	补料发酵	[50]
C. butyricum AKR102a	粗甘油	-	76.2	2.3	补料发酵	[52]
C. beijerinckii A1	粗甘油+玉米浆	0.54 g/g	20.0	0.71	分批发酵	[53]
C. pasteurianum MNO6	粗甘油	0.25 mol/mol		1.21	分批发酵	[54]
L. brevis N1E9.3.3	粗甘油+葡萄糖	0.83 g/g	18.6	0.45	分批发酵	[31]
L. reuteri HR2	粗甘油	0.51 mol/mol	9.1	-	分批发酵	[58]
菌群（梭菌属、乳酸杆菌属和肠杆菌属占据95%）	粗甘油（无长链脂肪酸）	0.44 g/g	-	-	分批发酵	[60]
菌群C2-2M	粗甘油	0.45 g/g	57.86	5.55	分批发酵	[61]
菌群DL38	粗甘油	0.63 mol/mol	81.4	0.99	分批发酵	[62]
菌群DL38-BH	粗甘油	0.60 mol/mol	31.17	-	分批发酵	[63]

菌株或菌群 Strain or microbial consortium	底物 Substrate	产率 Yield	产量Concent-ration/（g·L^-1）	生产强度Produc- tivity/（g·L^-1·h^-1）	发酵方式 Fermentation	文献 Reference
K. pneumoniae ATCC 8724	粗甘油	0.65 mol/mol	20	-	补料发酵	[48]
K. pneumoniae 2e	粗甘油	0.64 mol/mol	10.28	-	分批发酵	[46]
K. pneumoniae ATCC 200721 ∆ldhA	粗甘油	-	81.1	3.38	补料发酵	[50]
C. butyricum AKR102a	粗甘油	-	76.2	2.3	补料发酵	[52]
C. beijerinckii A1	粗甘油+玉米浆	0.54 g/g	20.0	0.71	分批发酵	[53]
C. pasteurianum MNO6	粗甘油	0.25 mol/mol		1.21	分批发酵	[54]
L. brevis N1E9.3.3	粗甘油+葡萄糖	0.83 g/g	18.6	0.45	分批发酵	[31]
L. reuteri HR2	粗甘油	0.51 mol/mol	9.1	-	分批发酵	[58]
菌群（梭菌属、乳酸杆菌属和肠杆菌属占据95%）	粗甘油（无长链脂肪酸）	0.44 g/g	-	-	分批发酵	[60]
菌群C2-2M	粗甘油	0.45 g/g	57.86	5.55	分批发酵	[61]
菌群DL38	粗甘油	0.63 mol/mol	81.4	0.99	分批发酵	[62]
菌群DL38-BH	粗甘油	0.60 mol/mol	31.17	-	分批发酵	[63]