不同多年生稻品种内生细菌群落多样性比较分析

doi:10.13560/j.cnki.biotech.bull.1985.2023-1088

摘要/Abstract

摘要：

【目的】为了解多年生稻内生细菌微生态的结构和潜在功能，探究多年生稻内生细菌与寄主优良性状的关系。【方法】通过16S rRNA扩增子测序技术比较分析了不同多年生稻品种（PR23、PR25和PR107）内生细菌多样性、物种组成和代谢功能。【结果】不同基因型的多年生稻具有不同的内生细菌群落，三者β多样性存在显著差异。多年生稻地下和地上部组织分化形成了不同的内生细菌群落，其地下部内生细菌主要由变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）、拟杆菌门（Bacteroidetes）等组成，而地上部内生细菌主要由变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、厚壁菌门（Firmicutes）等组成。不同多年生稻内生细菌特有扩增子序列变体（amplicon sequence variants, ASVs）数量较多，且具有不同的优势内生细菌属和生物标记物种类。多年生稻内生细菌的代谢功能主要为生物合成、生物降解与利用、解毒、代谢产物前体和能量的产生、甘氨酸途径、大分子修饰和代谢簇。品种间内生细菌的差异代谢功能主要涉及酯类、醇类、糖类等物质的生物合成和降解。【结论】寄主基因型和组织分化影响了多年生稻内生细菌的群落结构、物种组成和代谢功能。

关键词: 多年生稻, 内生细菌, 群落结构, 16S rRNA测序

Abstract:

【Objective】To explore the structure and potential functions of endophytic bacterial microbiota in perennial rice, as well as the relationship between endophytic bacteria in perennial rice and host agronomic traits. 【Method】The diversity, composition and metabolic function of endophytic bacteria in different in perennial rice varieties（PR23, PR25, and PR107）were characterized via Illumina sequencing of the 16S rRNA gene. 【Result】These results showed that the beta diversity of endophytic bacterial communities significantly differed among the distinct genotypes of perennial rice. Due to the differentiation of tissue structures, perennial rice varieties shaped distinctive ecological niches for endophytes in different tissue compartments. Proteobacteria, Firmicutes and Bacteroidetes were the dominant endophytic bacterial phyla in the underground tissues of perennial rice, while Proteobacteria, Actinobacteria and Firmicutes were the dominant phyla in the aboveground tissues. These perennial rice varieties had different endophytic bacterial taxa with specific amplicon sequence variants（ASVs）, dominant genera and specific biomarkers. The endophytic bacterial communities of perennial rice were reassembled for various metabolic functions, including biosynthesis, degradation/utilization/assimilation, detoxification, generation of precursor metabolite and energy, glycan pathways, macromolecule modification and metabolic clusters. Significantly different MetaCyc pathways of endophytic bacteria in perennial rice varieties with distinct genotypes were involved in the biosynthesis and degradation of esters, alcohols and polysaccharides. 【Conclusion】These results indicated host genotypes and tissues differentiation affect the community structure, species composition and metabolic function of endophytic bacteria in perennial rice.

Key words: perennial rice, endophytic bacteria, community structure, 16S rRNA sequencing

孔德婷, 齐笑含, 刘兴蕾, 李丽萍, 胡凤益, 黄立钰, 秦世雯. 不同多年生稻品种内生细菌群落多样性比较分析[J]. 生物技术通报, 2024, 40(5): 225-236.

KONG De-ting, QI Xiao-han, LIU Xing-lei, LI Li-ping, HU Feng-yi, HUANG Li-yu, QIN Shi-wen. Comparison and Analysis of Endophytic Bacterial Communities in Different Perennial Rice Varieties[J]. Biotechnology Bulletin, 2024, 40(5): 225-236.

图/表 5

图1 多年生稻品种地上和地下部分内生细菌群落α和β多样性

Fig. 1 α and β diversity of endophytic bacterial communities in the aboveground and the underground compartments of perennial rice varieties PR23 and PR25 are japonica subspecies, and PR107 is indica subspecies

图2 多年生稻品种地上和地下部分内生细菌在门水平下的物种组成（A）以及共有和特有内生细菌分类单元（ASV）（B）

Fig. 2 Composition of endophytic bacteria at phyla level in the aboveground and the underground compartments（A）of perennial rice varieties as well as the common and variety-specific endophytic bacterial ASVs in perennial rice（B）

图3 多年生稻品种地上和地下部分相对丰度在Top 20的内生细菌属（A）和标志性内生细菌（B）

Fig. 3 Endophytic bacterial genera（A）and biomarking endophytic bacteria（B）with top 20 of relative abundances in the aboveground and the underground compartments of perennial rice varieties

图4 多年生稻品种地上和地下部分内生细菌的MetaCyc代谢通路

Fig. 4 MetaCyc pathways of endophytic bacteria in the aboveground and the background compartments of perennial rice varieties

图5 多年生稻品种地上和地下部分内生细菌的差异MetaCyc代谢通路

Fig. 5 Significantly differential MetaCyc pathways enriched in the aboveground and the belowground compartments of perennial rice

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