曙厉蝽和益蝽线粒体基因组全序列特征及系统发育分析

doi:10.13560/j.cnki.biotech.bull.1985.2024-0387

摘要/Abstract

摘要：

【目的】曙厉蝽（Eocanthecona concinna）和益蝽（Picromerus lewisi）是农林业害虫重要的捕食性天敌昆虫资源，为了解其线粒体基因组的特点与差异，探究益蝽亚科昆虫的系统发育关系，对两种蝽类天敌昆虫的线粒体基因组序列进行测定及分析。【方法】通过测序后拼接、校正、注释获得两种蝽的线粒体全基因组序列，并利用最大似然法构建了基于益蝽亚科昆虫13个蛋白质编码基因（protein-coding gene, PCG）序列的系统发育树。【结果】曙厉蝽和益蝽线粒体基因组大小分别为18 740 bp（GenBank登录号：OR979468）和17 181 bp（GenBank登录号：OR972558），均包括13个PCG、2个核糖体RNA基因（rRNA）、22个转运RNA基因（tRNA）和1个控制区，基因排列与典型的蝽科昆虫相同。曙厉蝽和益蝽线粒体基因组控制区均位于rrnS和trnI之间，长度分别为3 862和2 438 bp，结构差异较大，均包含了不同长度的随机复制区域。对比益蝽亚科昆虫的线粒体基因组全序列和PCG序列的相似度，结果显示同属之间相似度较高，益蝽不同样本PCG序列的相似度超过98%。系统发育分析结果表明益蝽属（Picromerus）的昆虫聚为一支，曙厉蝽、叉角曙厉蝽（E. furcellata）、黑曙厉蝽（E. thomsoni）（GenBank登录号：OP920755）聚为一支，曙厉蝽属与益蝽属的亲缘关系最近，两个属与疣蝽属（Cazira）亲缘关系较远。【结论】明确了两种蝽类天敌昆虫线粒体基因组的特征，构建了益蝽亚科昆虫的系统发育关系，结果与传统分类学鉴定结果一致。

关键词: 曙厉蝽, 益蝽, 线粒体基因组, 益蝽亚科, 系统发育

Abstract:

【Objective】Eocanthecona concinna and Picromerus lewisi are important predatory natural enemy resources of agricultural and forestry pests in China. It is aimed to investigate their characteristics and differences of mitochondrial genome and explore the phylogenetic relationship of Asopinae insects. The mitochondrial genome sequence of two main insect species were determined and analyzed.【Method】The mitochondrial genome sequences of two enemies were determined and analyzed. After sequencing, the complete mitochondrial genome sequences of E. concinna and P. lewisi were obtained by splicing, correcting and annotating, and the phylogenetic trees of Asopinae insects were constructed based on the protein nucleotide and amino acid sequences using the maximum likelihood method.【Result】The complete mitochondrial genome sequences of E. concinna and P. lewisi were 18 740 bp（GenBank accession No. OR979468）and 17 181 bp（GenBank accession No. OR972558）in size, respectively. Including 13 protein-coding genes（PCGs）, two ribosomal RNA genes（rRNAs）, 22 transfer RNA genes（tRNAs）, and a control region. The same gene arrangement of the two enemies is consistent with the typical Pentatomidae insects. The control regions of the mitochondrial genomes of E. concinna and P. lewisi were located between rrnS and trnI, with lengths of 3 862 and 2 438 bp, respectively. The structures were different, and both contained random replication regions of different lengths. By comparing the similarity of the full sequence and PCGs of the mitochondrial genomes with other Asopinae insects, the results showed that the similarity between the same genus was high, and PCGs similarity between different geographical populations of P. lewisi was more than 98%. Phylogenetic analysis showed that the Picromerus species were clustered together and E. concinna, E. furcellata, E. thomsoni（GenBank accession No. OP920755）were clustered into one branch. The two genera, Eocanthecon and Picromerus, have close relationship to each other, and far relationships between Cazira and Eocanthecon or Picromerus.【Conclusion】The mitochondrial genome characteristics of E. concinna and P. lewisi have been clarified in this study, and the phylogenetic relationship of Asopinae bugs has been constructed, which was consistent with the results of traditional taxonomic identification.

Key words: Eocanthecona concinna, Picromerus lewisi, mitochondrial genome, Asopinae, phylogeny

江宏燕, 陈世春, 廖姝然, 陈亭旭, 王晓庆. 曙厉蝽和益蝽线粒体基因组全序列特征及系统发育分析[J]. 生物技术通报, 2025, 41(1): 312-323.

JIANG Hong-yan, CHEN Shi-chun, LIAO Shu-ran, CHEN Ting-xu, WANG Xiao-qing. The Complete Sequences and Phylogenetic Analysis of Mitochondrial Genomes in Eocanthecona concinna and Picromerus lewisi[J]. Biotechnology Bulletin, 2025, 41(1): 312-323.

图/表 9

参考文献 32

[1]	唐艺婷, 王孟卿, 李玉艳, 等. 捕食性蝽防治草地贪夜蛾的研究进展[J]. 中国生物防治学报, 2019, 35(5): 682-690. doi: 10.16409/j.cnki.2095-039x.2019.05.014
	Tang YT, Wang MQ, Li YY, et al. Research advances of predatory bugs to Spodoptera frugiperda[J]. Chin J Biol Contr, 2019, 35(5): 682-690.
[2]	唐艺婷. 一种新天敌——益蝽的生物防治潜能研究[D]. 北京: 中国农业科学院, 2020.
	Tang YT. Study on potential of a novel natural enemy insect Picromerus lewisi Scott in biological control[D]. Beijing: Chinese Academy of Agricultural Sciences, 2020.
[3]	唐美君, 王志博, 张欣欣, 等. 茶园新天敌绿点益蝽的初步研究[J]. 植物保护, 2023, 49(3): 231-235.
	Tang MJ, Wang ZB, Zhang XX, et al. Picromerus viridipunctatus Yang: a new natural enemy in tea plantations[J]. Plant Prot, 2023, 49(3): 231-235.
[4]	陈琴. 植食性和捕食性蝽线粒体基因组比较研究[D]. 贵阳: 贵州师范大学, 2022.
	Chen Q. Comparative study on the mitochondrial genomes of phytophagous and predatory stink bugs[D]. Guiyang: Guizhou Normal University, 2022.
[5]	魏书军, 陈学新. 昆虫比较线粒体基因组学研究进展[J]. 应用昆虫学报, 2011, 48(6): 1573-1585.
	Wei SJ, Chen XX. Progress in research on the comparative mitogenomics of insects[J]. Chin J Appl Entomol, 2011, 48(6): 1573-1585.
[6]	王维, 孟智启, 石放雄, 等. 鳞翅目昆虫比较线粒体基因组学研究进展[J]. 科学通报, 2013, 58(30): 3017-3029.
	Wang W, Meng ZQ, Shi FX, et al. Advances in comparative mitogenomic studies of Lepidoptera(Arthropoda: Insecta)[J]. Chin Sci Bull, 2013, 58(30): 3017-3029.
[7]	赵玲. 蝽科四亚科代表物种的比较线粒体基因组学研究[D]. 太谷: 山西农业大学, 2021.
	Zhao L. Study on the comparative mitochondrial genomes of representative species of four subfamilies of Pentatomidae(Hemiptera: Pentatomoidea)[D]. Taigu: Shanxi Agricultural University, 2021.
[8]	廉丹, 魏久锋, 丁晓飞, 等. 蝽科线粒体基因组tRNA和rRNA基因的比较及应用(半翅目: 蝽总科)[J]. 福建农林大学学报: 自然科学版, 2022, 51(6): 782-791.
	Lian D, Wei JF, Ding XF, et al. Comparison and application of tRNA and rRNA genes in the mitochondrial genome of Pentatomidae(Hemiptera: Pentatomoidea)[J]. J Fujian Agric For Univ Nat Sci Ed, 2022, 51(6): 782-791.
[9]	李荣荣, 李敏, 孙珊珊, 等. 红角辉蝽和紫翅果蝽线粒体完整基因组测序及蝽亚科系统发育分析(半翅目:蝽科)(英文)[J]. 昆虫学报, 2022, 65(10): 1343-1353.
	Li RR, Li M, Sun SS, et al. Sequencing of the complete mitochondrial genomes of Carbula crassiventris and Carpocoris purpureipennis and phylogenetic analysis of Pentatominae(Hemiptera: Pentatomidae)[J]. Acta Entomologica Sinca, 2022, 65(10): 1343-1353.
[10]	李根丽, 王宇宸, 刘鑫阳, 等. 褐兜蝽线粒体全基因组序列及系统发育分析[J]. 福建农林大学学报: 自然科学版, 2023, 52(2): 149-159.
	Li GL, Wang YC, Liu XY, et al. Complete mitochondrial genome and phylogenetic analysis of Coridius brunneus[J]. J Fujian Agric For Univ Nat Sci Ed, 2023, 52(2): 149-159.
[11]	Zhao Q, Wei JF, Zhao WQ, et al. The first mitochondrial genome for the subfamily asopinae(Heteroptera: Pentatomidae)and its phylogenetic implications[J]. Mitochondrial DNA B Resour, 2017, 2(2): 804-805.
[12]	Mu YL, Zhang CH, Zhang YJ, et al. Characterizing the complete mitochondrial genome of Arma custos and Picromerus lewisi(Hemiptera: Pentatomidae: asopinae)and conducting phylogenetic analysis[J]. J Insect Sci, 2022, 22(1): 6.
[13]	Chen XH, Leng M, Yao DH, et al. The complete mitochondrial genome of Picromerus lewisi Scott 1874(Hemiptera: Pentatomidae: asopinae)[J]. Mitochondrial DNA B Resour, 2023, 8(2): 285-287.
[14]	Yuan ML, Zhang QL, Guo ZL, et al. Comparative mitogenomic analysis of the superfamily pentatomoidea(Insecta: Hemiptera: Heteroptera)and phylogenetic implications[J]. BMC Genomics, 2015, 16(1): 460.
[15]	赵清. 中国益蝽亚科修订及蠋蝽属、辉蝽属和二星蝽属的DNA分类学研究(半翅目: 蝽科)[D]. 天津: 南开大学, 2013.
	Zhao Q. A revision of the Asopinae from China and the study of DNA taxonomy of Arma, Carbula and Eysarcoris(Hemipetra: Pentatomidae)[D]. Tianjin: Nankai University, 2013.
[16]	Thomas DB. Taxonomic synopsis of the old world asopine Genera(Heteroptera: Pentatomidae)[J]. Insect Mundi, 1994, 8: 145-212.
[17]	Thomas DB. Taxonomic synopsis of the asopinae Pentatomidae(Heteroptera)of thewestemn Hemisphere[M]. Lanham: Thomas Say foundation monographs - Entomological Society of American, 1992.
[18]	Brugnera R, Campos LA, Grazia J. Revision of Tynacantha Dallas with description of two new species(Hemiptera: Heteroptera: Pentatomidae: asopinae)[J]. Zootaxa, 2019, 4656(3): 445-458. doi: 10.11646/zootaxa.4656.3.3
[19]	David AD, 郑乐怡. 中国蝽科名录与有关学名变动I.益蝽亚科(半翅目: 异翅亚目)[J]. 昆虫分类学报, 2002, 24(2): 107-115.
	David AD, Zheng YY. Checklist and nomenclatural notes on the Chinese Pentatomidae(Heteroptera)I. Asopinae[J]. Entomotaxonomia, 2002, 24(2): 107-115.
[20]	曾明森, 吴光远, 王庆森. 茶园害虫捕食性天敌——厉蝽的生物学特性初步研究[J]. 福建农业学报, 2004, 19(3): 137-139.
	Zeng MS, Wu GY, Wang QS. Biological characteristics of Can-theconidea concinna Walker, a natural enemy to pests in tea garden[J]. Fujian J Agric Sci, 2004, 19(3): 137-139.
[21]	曾明森, 吴光远, 王庆森. 茶园天敌厉蝽的初步研究[J]. 武夷科学, 2003, 19(0): 76-79.
	Zeng MS, Wu GY, Wang QS. Preliminary studies on Cantheconidea concinna walker[J]. Wuyi Sci J, 2003, 19(0): 76-79.
[22]	Lai Y, Li KY, Liu XY. Comprehensive DNA barcode reference library and optimization of genetic divergence threshold facilitate the exploration of species diversity of green lacewings(Neuroptera: Chrysopidae)[J]. Insect Sci, 2024, 31(2): 613-632.
[23]	Li BY, Duan YG, Du ZY, et al. Natural selection and genetic diversity maintenance in a parasitic wasp during continuous biological control application[J]. Nat Commun, 2024, 15(1): 1379. doi: 10.1038/s41467-024-45631-2 pmid: 38355730
[24]	Li HR, Peng Y, Wang YS, et al. Global patterns of genomic and phenotypic variation in the invasive harlequin ladybird[J]. BMC Biol, 2023, 21(1): 141. doi: 10.1186/s12915-023-01638-7 pmid: 37337183
[25]	Bernt M, Donath A, Jühling F, et al. MITOS: improved de novo metazoan mitochondrial genome annotation[J]. Mol Phylogenet Evol, 2013, 69(2): 313-319.
[26]	Lowe TM, Chan PP. tRNAscan-SE on-line: integrating search and context for analysis of transfer RNA genes[J]. Nucleic Acids Res, 2016, 44(W1): W54-W57.
[27]	Lefort V, Longueville JE, Gascuel O. SMS: smart model selection in PhyML[J]. Mol Biol Evol, 2017, 34(9): 2422-2424. doi: 10.1093/molbev/msx149 pmid: 28472384
[28]	Zhang D, Gao FL, Jakovlić I, et al. PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies[J]. Mol Ecol Resour, 2020, 20(1): 348-355. doi: 10.1111/1755-0998.13096 pmid: 31599058
[29]	Nguyen LT, Schmidt HA, von Haeseler A, et al. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies[J]. Mol Biol Evol, 2015, 32(1): 268-274.
[30]	Chen SC, Jiang HY, Liao SR, et al. Complete mitochondrial genome of Stethoconus japonicus(Hemiptera: Miridae): insights into the evolutionary traits within the family Miridae[J]. Gene, 2024, 891: 147830.
[31]	Zhang H, Lu CC, Liu Q, et al. Insights into the evolution of aphid mitogenome features from new data and comparative analysis[J]. Animals, 2022, 12(15): 1970.
[32]	丁晓飞, 魏久锋, 高晓云, 等. 天敌昆虫蠋蝽线粒体基因组蛋白编码基因分析[J]. 山西农业科学, 2023, 51(2): 123-132.
	Ding XF, Wei JF, Gao XY, et al. Analysis of protein coding genes in mitochondrial genome of a natural enemy, Arma custos[J]. J Shanxi Agric Sci, 2023, 51(2): 123-132.

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基因 Gene	基因位置Gene position/bp		基因长度Gene length/bp		基因间隔Intergenic space/bp		起始密码子/终止密码子Start codon/Stop codon
基因 Gene	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi
cox1	1-1 537	1-1 537	1 537	1 537	15	7	TTG/T-	TTG/T-
trnL2	1 538-1 602	1 538-1 601	65	64	0	0
cox2	1 603-2 281	1 602-2 280	679	679	0	0	ATA/T-	ATA/T-
trnK	2 282-2 354	2 281-2 354	73	74	0	0
trnD	2 354-2 420	2 358-2 424	67	67	-1	3
atp8	2 421-2 582	2 425-2 583	162	159	0	0	TTG/TAA	GTG/TAA
atp6	2 576-3 250	2 577-3 251	675	675	-7	-7	ATG/TAA	ATG/TAA
cox3	3 256-4 044	3 259-4 047	789	789	5	7	ATG/TAA	ATG/TAA
trnG	4 066-4 130	4 049-4 112	65	64	21	1
nad3	4 131-4 484	4 114-4 467	354	354	0	1	ATC/TAA	ATA/TAA
trnA	4 497-4 560	4 487-4 555	64	69	12	19
trnR	4 590-4 654	4 562-4 628	65	67	29	6
trnN	4 658-4 724	4 635-4 702	67	68	3	6
trnS1	4 731-4 800	4 702-4 771	70	70	6	-1
trnE	4 800-4 865	4 771-4 836	66	66	-1	-1
trnF	4 882-4 946	4 837-4 904	65	68	16	0
nad5	4 947-6 654	4 915-6 624	1 708	1 710	0	10	ATT/T-	ATT/TAA
trnH	6 656-6 726	6 626-6 694	71	69	1	1
nad4	6 729-8 060	6 699-8 027	1 332	1 329	2	4	ATG/TAA	ATG/TAA
nad4L	8 054-8 341	8 021-8 308	288	288	-7	-7	ATT/TAA	ATT/TAA
trnT	8 344-8 407	8 311-8 377	64	67	2	2
trnP	8 408-8 471	8 378-8 441	64	64	0	0
nad6	8 473-8 952	8 446-8 925	480	480	1	4	ATG/TAA	ATG/TAA
cob	8 968-10 107	8 933-10 072	1 140	1 140	15	7	ATG/TAA	ATG/TAG
trnS2	10 119-10 187	10 075-10 143	69	69	11	2
nad1	10 216-11 136	10 169-11 089	921	921	28	25	TTG/TAA	TTG/TAA
trnL1	11 137-11 202	11 090-11 156	66	67	0	0
rrnL	11 203-12 482	11 157-12 449	1 280	1 293	0	0
trnV	12 483-12 550	12 450-12 517	68	68	0	0
rrnS	12 551-13 343	12 518-13 318	793	801	0	0
CR	13 344-17 205	13 319-15 756	3 862	2 438	0	0
trnI	17 206-17 272	15 757-15 823	67	67	0	0
trnQ	17 374-17 442	15 827-15 897	69	71	101	3
trnM	17 451-17 518	15 912-15 982	68	71	8	14
nad2	17 538-18 500	15 983-16 963	963	981	19	0	ATA/TAG	ATT/TAA
trnW	18 520-18 585	16 981-17 047	66	67	19	17
trnC	18 591-18 653	17 040-17 102	63	63	5	-8
trnY	18 661-18 725	17 108-17 174	65	67	7	5

基因 Gene	基因位置Gene position/bp		基因长度Gene length/bp		基因间隔Intergenic space/bp		起始密码子/终止密码子Start codon/Stop codon
基因 Gene	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi
cox1	1-1 537	1-1 537	1 537	1 537	15	7	TTG/T-	TTG/T-
trnL2	1 538-1 602	1 538-1 601	65	64	0	0
cox2	1 603-2 281	1 602-2 280	679	679	0	0	ATA/T-	ATA/T-
trnK	2 282-2 354	2 281-2 354	73	74	0	0
trnD	2 354-2 420	2 358-2 424	67	67	-1	3
atp8	2 421-2 582	2 425-2 583	162	159	0	0	TTG/TAA	GTG/TAA
atp6	2 576-3 250	2 577-3 251	675	675	-7	-7	ATG/TAA	ATG/TAA
cox3	3 256-4 044	3 259-4 047	789	789	5	7	ATG/TAA	ATG/TAA
trnG	4 066-4 130	4 049-4 112	65	64	21	1
nad3	4 131-4 484	4 114-4 467	354	354	0	1	ATC/TAA	ATA/TAA
trnA	4 497-4 560	4 487-4 555	64	69	12	19
trnR	4 590-4 654	4 562-4 628	65	67	29	6
trnN	4 658-4 724	4 635-4 702	67	68	3	6
trnS1	4 731-4 800	4 702-4 771	70	70	6	-1
trnE	4 800-4 865	4 771-4 836	66	66	-1	-1
trnF	4 882-4 946	4 837-4 904	65	68	16	0
nad5	4 947-6 654	4 915-6 624	1 708	1 710	0	10	ATT/T-	ATT/TAA
trnH	6 656-6 726	6 626-6 694	71	69	1	1
nad4	6 729-8 060	6 699-8 027	1 332	1 329	2	4	ATG/TAA	ATG/TAA
nad4L	8 054-8 341	8 021-8 308	288	288	-7	-7	ATT/TAA	ATT/TAA
trnT	8 344-8 407	8 311-8 377	64	67	2	2
trnP	8 408-8 471	8 378-8 441	64	64	0	0
nad6	8 473-8 952	8 446-8 925	480	480	1	4	ATG/TAA	ATG/TAA
cob	8 968-10 107	8 933-10 072	1 140	1 140	15	7	ATG/TAA	ATG/TAG
trnS2	10 119-10 187	10 075-10 143	69	69	11	2
nad1	10 216-11 136	10 169-11 089	921	921	28	25	TTG/TAA	TTG/TAA
trnL1	11 137-11 202	11 090-11 156	66	67	0	0
rrnL	11 203-12 482	11 157-12 449	1 280	1 293	0	0
trnV	12 483-12 550	12 450-12 517	68	68	0	0
rrnS	12 551-13 343	12 518-13 318	793	801	0	0
CR	13 344-17 205	13 319-15 756	3 862	2 438	0	0
trnI	17 206-17 272	15 757-15 823	67	67	0	0
trnQ	17 374-17 442	15 827-15 897	69	71	101	3
trnM	17 451-17 518	15 912-15 982	68	71	8	14
nad2	17 538-18 500	15 983-16 963	963	981	19	0	ATA/TAG	ATT/TAA
trnW	18 520-18 585	16 981-17 047	66	67	19	17
trnC	18 591-18 653	17 040-17 102	63	63	5	-8
trnY	18 661-18 725	17 108-17 174	65	67	7	5

基因区域 Gene region	A/%		T/%		C/%		G/%		A+T/%		AT-偏斜AT-skew		GC-偏斜GC-skew
基因区域 Gene region	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi
全基因组 Whole genome	40.52	40.80	33.94	32.97	14.47	15.31	11.07	10.91	74.46	73.77	0.088	0.106	-0.133	-0.168
蛋白质编码基因PCG	31.47	31.76	41.12	40.86	13.91	13.81	13.49	13.57	72.60	72.62	-0.133	-0.125	-0.015	-0.009
1st codon	34.50	35.36	34.88	34.30	12.26	12.14	18.35	18.20	69.39	69.66	-0.005	0.015	0.199	0.200
2nd codon	21.03	20.73	45.82	45.76	18.48	18.29	14.67	15.22	66.86	66.49	-0.371	-0.376	-0.115	-0.092
3rd codon	38.88	39.18	42.67	42.53	10.99	11.01	7.46	7.28	81.55	81.71	-0.046	-0.041	-0.192	-0.204
tRNA	38.58	37.79	37.08	36.99	11.11	11.57	13.22	13.65	75.66	74.78	0.020	0.011	0.087	0.083
rRNA	33.77	32.81	43.37	43.94	8.25	8.31	14.62	14.95	77.13	76.74	-0.124	-0.145	0.278	0.285

基因区域 Gene region	A/%		T/%		C/%		G/%		A+T/%		AT-偏斜AT-skew		GC-偏斜GC-skew
基因区域 Gene region	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi	曙厉蝽 E. concinna	益蝽 P. lewisi
全基因组 Whole genome	40.52	40.80	33.94	32.97	14.47	15.31	11.07	10.91	74.46	73.77	0.088	0.106	-0.133	-0.168
蛋白质编码基因PCG	31.47	31.76	41.12	40.86	13.91	13.81	13.49	13.57	72.60	72.62	-0.133	-0.125	-0.015	-0.009
1st codon	34.50	35.36	34.88	34.30	12.26	12.14	18.35	18.20	69.39	69.66	-0.005	0.015	0.199	0.200
2nd codon	21.03	20.73	45.82	45.76	18.48	18.29	14.67	15.22	66.86	66.49	-0.371	-0.376	-0.115	-0.092
3rd codon	38.88	39.18	42.67	42.53	10.99	11.01	7.46	7.28	81.55	81.71	-0.046	-0.041	-0.192	-0.204
tRNA	38.58	37.79	37.08	36.99	11.11	11.57	13.22	13.65	75.66	74.78	0.020	0.011	0.087	0.083
rRNA	33.77	32.81	43.37	43.94	8.25	8.31	14.62	14.95	77.13	76.74	-0.124	-0.145	0.278	0.285

	曙厉蝽 E. concinna	益蝽1 P. lewisi 1	益蝽2 P. lewisi 2	益蝽3 P. lewisi 3	益蝽4 P. lewisi 4	叉角曙厉蝽E. furcellata	黑曙厉蝽1 E. thomsoni 1	黑曙厉蝽2 E. thomsoni 2	黑益蝽 P. griseus	绿点益蝽P. viridipunctatus	欧亚绿蝽 A. custos	朝鲜镯蝽 A. koreana	峰疣蝽 C. horvathi	疣蝽C. verrucosa	奥缘蝽D. dybowskyi	蓝蝽 Z. caerulea
曙厉蝽 E. concinna	——	71.53	71.58	68.64	73.60	70.88	68.32	63.87	67.45	68.94	67.22	66.12	67.49	66.62	67.10	66.39
益蝽1 P. lewisi 1	81.24	——	85.62	93.24	91.79	76.22	74.45	69.99	76.57	78.70	73.59	72.61	73.53	72.62	74.18	72.26
益蝽2 P. lewisi 2	81.13	98.16	——	81.18	88.45	66.73	64.99	61.46	66.98	68.67	64.35	63.50	64.57	63.73	64.77	63.24
益蝽3 P. lewisi 3	81.17	99.80	98.17	——	85.41	81.10	79.46	74.52	79.66	82.21	78.58	77.48	78.74	77.21	78.42	76.98
益蝽4 P. lewisi 4	81.07	98.43	98.60	98.47	——	72.41	70.22	66.41	72.21	74.37	69.73	68.78	69.73	68.75	70.04	68.45
叉角曙厉蝽 E. furcellata	83.23	82.59	82.36	82.54	82.18	——	81.44	75.76	78.23	80.49	79.90	78.94	80.06	78.48	79.54	78.88
黑曙厉蝽1 E. thomsoni 1	81.59	81.34	81.48	81.24	81.35	83.41	——	75.71	76.37	78.10	79.55	78.47	78.72	77.88	77.99	78.15
黑曙厉蝽2 E. thomsoni 2	81.43	81.39	81.41	81.36	81.47	82.46	81.42	——	72.43	74.39	76.89	75.80	75.15	74.53	74.48	77.96
黑益蝽P. griseus	79.26	81.68	81.83	81.66	81.71	80.31	79.47	79.78	——	81.15	75.61	74.68	76.13	75.69	76.66	74.75
绿点益蝽 P. viridipunctatus	81.39	84.39	84.04	84.32	84.03	82.99	81.26	82.21	82.45	——	77.35	76.36	78.03	77.59	78.31	76.54
欧亚绿蝽 A. custos	81.71	81.57	81.60	81.52	81.51	83.06	81.50	81.90	79.48	81.70	——	86.40	79.22	77.91	78.89	82.62
朝鲜镯蝽 A. koreana	80.14	80.29	80.23	80.21	80.06	81.74	80.19	80.66	78.18	80.36	86.97	——	78.19	77.30	77.57	81.18
峰疣蝽 C. horvathi	80.84	81.23	81.14	81.15	81.10	82.33	80.66	81.88	79.44	81.61	82.45	81.19	——	80.73	79.20	78.88
疣蝽C. verrucosa	79.94	79.57	79.53	79.48	79.52	80.94	79.48	81.08	78.56	80.53	80.93	80.06	82.22	——	78.52	77.33
奥缘蝽 D. dybowskyi	79.84	80.32	80.65	80.25	80.64	80.99	79.67	80.61	78.27	80.08	81.29	79.64	80.98	80.21	——	77.78
蓝蝽Z. caerulea	81.33	81.07	81.19	81.01	81.03	83.00	81.14	81.85	79.41	81.69	84.44	83.04	82.81	81.18	81.11	——