纳豆激酶生产菌BSNK-5 鉴定及发酵条件优化

生物技术通报 ›› 2013, Vol. 0 ›› Issue (2): 184-189.

纳豆激酶生产菌BSNK-5 鉴定及发酵条件优化

李淑英¹ 聂莹¹ 杜欢¹ 赵仲麟² 袁超² 李燕³ 宋晓燕⁴ 唐选明¹

（1. 中国农业科学院农产品加工研究所，北京 100193; 2. 河南农业大学理学院，郑州 450002; 3. 郑州轻工业学院食品与生物工程学院，郑州 450002; 4. 河南农业大学食品科学技术学院，郑州 450002）

收稿日期:2012-09-29 修回日期:2013-02-27 出版日期:2013-02-26 发布日期:2013-02-27
作者简介:李淑英，女，博士，助理研究员，研究方向：微生物生化与分子生物学；E-mail ：lishuying@caas.net.cn
基金资助:
中国农业科学院科技经费项目（201211）

Identification of BSNK-5 and Optimization of the Fermentation Conditions

Li Shuying¹ Nie Ying¹ Du Huan¹ Zhao Zhonglin² Yuan Chao² Li Yan³ Song Xiaoyan⁴ Tang Xuanming¹

（1. Institute of Agro-products Processing Science and Technology，Chinese Academy of Agricultural Sciences，Beijing 100193 ；2. College of Sciences，Henan Agricultural University，Zhengzhou 450002 ；3. School of Food and Biological Engineering，Zhengzhou University of Light Industry，Zhengzhou 450002 ；4. College of Food Science and Technology，Henan Agricultural University，Zhengzhou 450002）

Received:2012-09-29 Revised:2013-02-27 Published:2013-02-26 Online:2013-02-27

摘要/Abstract

摘要： 对分离的纳豆激酶生产菌BSNK-5 进行初步鉴定；并对其液体发酵产酶条件进行了初步研究，旨在提高纳豆激酶的产量。通过鉴定BSNK-5 为枯草芽孢杆菌属菌株。试验优化了发酵培养基的最适氮源和碳源种类及百分含量，发酵培养基的起始pH 值，最适种子接种量，发酵温度，培养时间等。最终确定了两个最适的产酶条件，在该条件下，BSNK-5 通过液体发酵产纳豆激酶的活力高达1 160 U/mL，比优化前提高283%。

关键词: 枯草芽孢杆菌, 纳豆激酶, 液体发酵

Abstract: Nattokinase production bacteria BSNK-5 was preliminarily identified. The effects of liquid fermentation on nattokinase activity produced by BSNK-5 were studied in order to improve production yield. BSNK-5 is a Bacillus subtilis. Different carbon sources, nitrogen sources, pH, inoculate size；temperature, and time were optimized. Two optimum culture conditions were obtained. The nattokinase activity was 1 160 U/mL under the optimum culture conditions, which was 283% times of the former culture conditions.

Key words: Bacillus natto, Nattokinase, Liquid fermentation

李淑英, 聂莹, 杜欢, 赵仲麟, 袁超, 李燕, 宋晓燕, 唐选明. 纳豆激酶生产菌BSNK-5 鉴定及发酵条件优化[J]. 生物技术通报, 2013, 0(2): 184-189.

Li Shuying, Nie Ying, Du Huan, Zhao Zhonglin, Yuan Chao, Li Yan, Song Xiaoyan, Tang Xuanming. Identification of BSNK-5 and Optimization of the Fermentation Conditions[J]. Biotechnology Bulletin, 2013, 0(2): 184-189.

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