UV-B辐射对小麦叶片蛋白的影响

生物技术通报 ›› 2013, Vol. 0 ›› Issue (3): 53-59.

UV-B辐射对小麦叶片蛋白的影响

齐婷婷,段江燕

山西师范大学生命科学学院，临汾 041000

收稿日期:2012-09-26 修回日期:2013-03-21 出版日期:2013-03-20 发布日期:2013-03-21
作者简介:齐婷婷，女，硕士研究生，研究方向：植物信号转导和生物化学；E-mail ：361498415@qq.com
基金资助:
山西省自然科学基金项目（2009011044-2）

UV-B Radiation Affects Protein Expression of the Wheat’s Leaf

Qi Tingting, Duan Jiangyan

College of Life Science of Shanxi Normal University, Linfen 041000)

Received:2012-09-26 Revised:2013-03-21 Published:2013-03-20 Online:2013-03-21

摘要/Abstract

摘要： 为研究增强的UV-B 辐射对植物的影响，选取了生长于中国北方的经济作物冬小麦为研究对象，采用双向电泳的方法，分析了经UV-B 辐射后小麦叶片蛋白的变化。结果显示，经UV-B 辐射后，第4 天、第8 天的小麦叶片蛋白变化明显，双向电泳图谱显示发现15 个蛋白差异点；通过质谱鉴定了3 个蛋白差异点，分别为铜/ 锌过氧化物歧化酶、钙调素、Rubiso 大亚基结合蛋白α 亚基。结果表明增强的UV-B 辐射可以通过调节小麦叶片基因编码蛋白而调节植物生长。

关键词: UV-B辐射, 小麦叶片蛋白, 双向电泳

Abstract: The reduction in average global ozone layer may cause the enhance of solar ultradiation adiation（UV-B）that reach to the ground surface. We selected the cash crops of winter wheat which grown in northern China to studied the influence of UV-B radiation on plants. Using the method of two-dimensional electrophoresis to analysis changes of the protein in wheat leaves by UV-B radiation. The results showed that wheat leaf protein of the first four days, eight days changes significantly. The two dimensional gel electrophoresis revealed 15 differentially expressed polypeptides, 3 of which were successful identified by mass spectrometry, including Copper/Zinc Superoxide dismutase, Calmodulin, Rubisco large subunit binding protein subunit alpha. The results showed that enhanced UV-B radiation can be regulating the gene encoding protein of wheat leaf to adjust plant growth.

Key words: UV-B radiation, Protein of wheat leaves, Two-dimensional electrophoresis

齐婷婷,段江燕. UV-B辐射对小麦叶片蛋白的影响[J]. 生物技术通报, 2013, 0(3): 53-59.

Qi Tingting, Duan Jiangyan. UV-B Radiation Affects Protein Expression of the Wheat’s Leaf[J]. Biotechnology Bulletin, 2013, 0(3): 53-59.

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