RNA干扰及其在植物研究中的应用

摘要/Abstract

摘要： RNA 干扰（RNAi）是利用双链RNA 特异性地降解同源靶标mRNA，从而特异性地阻断相应基因表达的现象，它属于转录后水平的基因沉默（PTGS）机制。RNAi 广泛存在于生物体中，是2l 世纪的研究热点之一。近年来，RNAi 在植物功能基因组学研究中发挥着越来越重要的作用。在此对RNAi 的作用机制和特点，及其在植物基因功能分析、作物品种改良、抗病虫害和抗逆性等方面的研究进行概述，旨在为RNAi 在植物研究上的应用提供参考。

关键词: RNA干扰, 双链RNA, 基因沉默, 植物, 应用

Abstract: RNA interference（RNAi）belongs to the post-transcriptional gene silencing phenomenon in which double-stranded RNA triggers the degradation of homologous mRNA, thereby specific to suppression the corresponding gene expression. RNAi is a phenomenon that commonly existed in organisms, and it has become one of the hotspots of scientific research in 21st century. In recent years, RNAi plays a more and more important role in plant functional genomics. We reviewed characteristics and action mechanism of RNAi, as well as the research progress in aspects including the plant gene function analysis, the crop variety improvement and the resistance to disease or pests, in order to provide reference for RNAi application in plant research.

Key words: RNA interference, Double-stranded RNA, Gene silencing, Plant, Application

王婷婷,王丹丹,Rahman Laibi Chelab,康丹,游腾飞,眭安平,杨星勇. RNA干扰及其在植物研究中的应用[J]. 生物技术通报, 2013, 0(3): 48-52.

Wang Tingting, Wang Dandan, Rahman Laibi Chelab, Kang Dan, You Tengfei, Sui Anping, Yang Xingyong. Technology of RNAi and Its Application in Plant Research[J]. Biotechnology Bulletin, 2013, 0(3): 48-52.

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