生物技术通报 ›› 2013, Vol. 0 ›› Issue (10): 142-147.

• 研究报告 • 上一篇    下一篇

西沙野生诺尼种子内生细菌群落多样性的初步研究

刘洋1,李辉1,李金霞1,曹艳花1,姚粟1,白飞荣1,谭望桥2, 程池1   

  1. (1. 中国食品发酵工业研究院 中国工业微生物菌种保藏管理中心,北京 100027 ;2. 海南诺尼生物工程开发有限公司,海口 570125)
  • 收稿日期:2013-03-28 修回日期:2013-10-15 出版日期:2013-10-14 发布日期:2013-10-15
  • 作者简介:刘洋, 男, 博士, 工程师, 研究方向: 微生物分子生物学与生态学;E-mail :ly81150@163.com
  • 基金资助:

    中国食品发酵工业研究院科技发展基金( 博士基金) 项目(2012KJFZ-BS-01)

Investigation on Diversity of Endophytic Bacterial Community in Xisha Wild Noni(Morinda citrifolia L.)Seed

Liu Yang1,Li Hui1,Li Jinxia1,Cao Yanhua1,Yao Su1,Bai Feirong1,Tan Wangqiao2, Cheng Chi1,   

  1. (1. China Center of Industrial Culture Collection,China National Research Institute of Food and Fermentation Industries,Beijing 100027 ; 2. Hainan Noni Biological Engineering Development Co.,Ltd.,Haikou 570125)
  • Received:2013-03-28 Revised:2013-10-15 Published:2013-10-14 Online:2013-10-15

摘要:

通过构建16S rDNA 克隆文库的非培养方法对我国西沙野生诺尼种子内生细菌群落结构多样性进行研究,共涉及Proteobacteria 、Firmicutes 、Bacteroidetes 及Actinobacteria 四大类群,包含33 个属,42 个OTU, 优势菌属(丰度)依次为Enterob-acter sp.(17.55%)、Bacillus sp.(16.79%)、Acinetobacter sp.(6.11%)、Pseudomonas sp.(6.11%) 及Piscinibacter sp.(6.11%)。这是国内外首次利用非培养方法针对诺尼种子内生细菌群落多样性进行研究的报道。

关键词: 诺尼, 种子, 内生细菌, 群落多样性, 非培养方法

Abstract:

Using the 16S rDNA clone library technique, a priliminary study was conducted on the community diversity of endophytic bacteria in seeds of Xisha wild Noni. The results indicated that the endophytic bacterial communities contained 42 Operational Taxonomic Units (OTUs)in their clone libraries respectively, involving 33 genus from 4 groups of Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria.
The dominant bacterium(abundance)were Enterobacter sp.(17.55%), Bacillus sp.(16.79%), Acinetobacter sp.(6.11%), Pseudomonas sp.(6.11%)and Piscinibacter sp.(6.11%). This was the first report of the study on endophytic bacterial communities in the Noni seeds with culture-independent method.

Key words: Noni, Seed, Endophytic bacteria, Bacterial community, Culture-independent method