环介导等温扩增技术在转基因水稻Bt63检测中的应用

生物技术通报 ›› 2014, Vol. 0 ›› Issue (2): 69-74.

环介导等温扩增技术在转基因水稻Bt63检测中的应用

张明哲¹, 陈曦¹, 徐俊锋², 陈吴健¹, 吴蓉³, 吴志毅^1,

1.浙江省检验检疫科学技术研究院，杭州 310016；2.浙江省农业科学研究院，杭州 310021；3.杭州出入境检验检疫局，杭州 310012

收稿日期:2013-09-06 出版日期:2014-02-27 发布日期:2014-02-27
作者简介:张明哲，男，高级农艺师，研究方向：分子生物学和植物检疫；E-mail：mzzhang429@163.com
基金资助:
国家质检总局科技计划项目（2011IK249），浙江省科技厅公益性项目（2011C22065），浙江省科技厅重大专项（2011C12023），浙江出入境检验检疫局科研项目（ZK200958）

Specific Detection of Genetically Modified Rice Bt63 Using Loop-Mediated Isothermal Amplification

Zhang Mingzhe¹, Chen Xi¹, Xu Junfeng², Chen Wujian¹, Wu Rong³, Wu Zhiyi¹,

（1. Zhejiang Academy of Science and Technology for Inspection and Quarantine，Hangzhou 310016；2. Zhejiang Academy of Agricultural Sciences，Hangzhou 310021；3. Hangzhou Entry-Exit Inspection and Quarantine Bureau，Hangzhou 310012）

Received:2013-09-06 Published:2014-02-27 Online:2014-02-27

摘要/Abstract

摘要： 采用环介导等温扩增技术（Loop-Mediated Isothermal Amplification，LAMP）对转基因水稻Bt63进行应用检测。研究表明，环介导等温扩增技术检测转基因水稻Bt63特异性好，并且灵敏度达到0.01%（W/W），具有简便、快速、准确等特点，可以在进出口检验检疫、食品安全监测等领域进行应用。

关键词: 环介导等温扩增技术, 转基因水稻Bt63, 检测

Abstract: It focused on the specific detection of genetically modified rice Bt63 using loop-mediated isothermal amplification（LAMP）. The LAMP could effectively detect genetically modified rice Bt63 with high specificity and the sensitivity limit is 0.01%（W/W）. LAMP is a rapid and accurate method and would be applied to inspection, quarantine and food safety.

张明哲, 陈曦, 徐俊锋, 陈吴健, 吴蓉, 吴志毅. 环介导等温扩增技术在转基因水稻Bt63检测中的应用[J]. 生物技术通报, 2014, 0(2): 69-74.

Zhang Mingzhe, Chen Xi, Xu Junfeng, Chen Wujian, Wu Rong, Wu Zhiyi,. Specific Detection of Genetically Modified Rice Bt63 Using Loop-Mediated Isothermal Amplification[J]. Biotechnology Bulletin, 2014, 0(2): 69-74.

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