香菇dsRNA 病毒LeV 的RT-PCR 检测

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 147-150.

香菇dsRNA 病毒LeV 的RT-PCR 检测

郭杰^1,2,吴小平¹

1. 福建农林大学生命科学学院，福州 350002
2. 三门峡农业科学研究院，三门峡 472000

收稿日期:2013-09-23 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:郭杰，男，助理农艺师，硕士，研究方向: 食用菌病害；E-mail: gjfywx_2008@163.com
基金资助:
福建省科技厅自然科学基金项目(2013J01080)

RT-PCR Detection of dsRNA Virus LeV in Lentinula edodes

Guo Jie^1,2, Wu Xiaoping¹

1. College of Life Science，Fujian Agriculture and Forestry University，Fuzhou 350002;
2. Institute of SanMenxia Agriculture Science，Sanmenxia 472000

Received:2013-09-23 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 香菇病毒HKB(Lentinula edodes mycovirus HKB，LeV)是一种具有潜隐性特点的真菌病毒，广泛存在于香菇菌株中。#br#为了快速、准确地检测出LeV，根据LeV 病毒基因组序列(AB.429556.2)的信息，设计合成一对引物，对25 个香菇菌株进行RTPCR#br#检测，在20 个香菇菌株中分别扩增出LeV 病毒基因组特有的条带，在5 个香菇菌株中未能扩增出条带。通过对25 个香菇菌#br#株进行dsRNA 提取检测，结果也表明不存在扩增片段的菌株提取不到dsRNA，存在扩增片段的菌株能够提取得到dsRNA。因此建#br#立的RT-PCR 方法可以快速检测到香菇dsRNA 病毒LeV，能够对香菇的质量检测提供技术支持。

关键词: dsRNA, 真菌病毒, RT-PCR, 香菇

Abstract: Lentinula edodes mycovirus HKB(LeV)is a fungal virus with the potential symptoms and exists widely in Lentinula edodes strains. In order to detect LeV from abundant strains rapidly and accurately, a pair of primers were designed according to the sequence of LeV (AB.429556.2)and amplified by RT-PCR method. In a total of 25 strains, 20 strains were positive for LeV and 5 strains were negative. The result of dsRNA extraction also showed dsRNA were obtained in the positive strains and the negative strains were not. In conclusion, the study proved that RT-PCR is a rapid and sensitive method for detection of LeV in Lentinula edodes strains and can attribute to the control of strains quality.

Key words: dsRNA, Mycovirus, RT-PCR, Lentinula edodes

郭杰,吴小平. 香菇dsRNA 病毒LeV 的RT-PCR 检测[J]. 生物技术通报, 2014, 0(1): 147-150.

Guo Jie, Wu Xiaoping. RT-PCR Detection of dsRNA Virus LeV in Lentinula edodes[J]. Biotechnology Bulletin, 2014, 0(1): 147-150.

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