香蕉枯萎病菌全局性调控因子FocVeA基因的克隆及功能预测

生物技术通报 ›› 2014, Vol. 0 ›› Issue (10): 101-106.

香蕉枯萎病菌全局性调控因子FocVeA基因的克隆及功能预测

漆艳香, 张欣, 陆英, 张贺, 蒲金基, 喻群芳, 谢艺贤

中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海口 571101

收稿日期:2014-03-20 出版日期:2014-10-20 发布日期:2014-10-17
作者简介:漆艳香,女,博士,副研究员,研究方向：热带果树病害的诊断、病原学及防治
基金资助:
现代农业产业技术体系建设专项资金（CARS-32-04）,中央级公益性科研院所基本科研业务费专项资金（2013hzs1J009）

Cloning and Functional Prediction of the Global Regulator FocVeA Gene from Fusarium oxysporum f. sp. cubense

Qi Yanxiang, Zhang Xin, Lu Ying, Zhang He, Pu Jinji, Yu Qunfan, g Xie Yixian

Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests,Ministry of Agriculture,Environment and Plant Protection Institute,CATAS,Haikou 571101

Received:2014-03-20 Published:2014-10-20 Online:2014-10-17

摘要/Abstract

摘要： 根据轮枝样镰刀菌（Fusarium verticillioides）和藤仓镰刀菌（F. fujikuroi）veA同源基因相关序列设计引物,利用PCR与RT-PCR技术分别克隆了香蕉枯萎病菌2个生理小种的FocVeA基因序列和开放阅读框,同时对该基因的编码蛋白进行序列特征、系统发育与结构域分析。结果表明,2个生理小种的FocVeA基因（分别命名为F1VeA和F4VeA）的DNA片段全长分别为1 693 bp 和1 690 bp,开放阅读框分别为1 599 bp 和1 596 bp,分别编码532个和531个氨基酸。F1VeA和F4VeA基因预测氨基酸序列相似性均为99%,与GenBank中公布的Fusarium spp. veA基因氨基酸序列均有78%-99%相似性。系统聚类分析显示,F1VeA和F4VeA与GenBank中已登录的轮枝样镰刀菌（F. verticillioides）和藤仓镰刀菌（F. fujikuroi）等veA同源蛋白高度同源。蛋白质保守域搜索表明,FocVeA具有veA蛋白的功能域,属于veA蛋白家族的一员,推测该基因可能参与调控F. oxysporum f. sp. cubense的生长、毒素合成及致病性等。

关键词: 香蕉枯萎病菌, FocVeA基因, 克隆, 序列分析

Abstract: Based on the conserved amino acid sequence of veA homologs from F. verticillioides and F. fujikuroi, FocVeA gene and its open reading frame of F. oxysporum f. sp. cubense race 1 and race 4（Foc1 and Foc4）were cloned by PCR and RT-PCR. Sequences characterization, phylogenetic clustering and conserved domains on the two predicted proteins of FocVeA gene were also analyzed, respectively. The results showed that the complete DNA sequences of FocVeA from Foc1 and Foc4（named as F1VeA and F4VeA）were 1 693 bp and 1 690 bp, respectively. The cDNA of F1VeA and F4VeA were 1 599 bp and 1 596 bp, and encoded a deduced protein of 532 amino acid and 531 amino acid, respectively. The deduced amino acid sequences of F1VeA and F4VeA shared 99% similar to each other, and had 78%-99% similarity with the sequences of veA factor from isolates of Fusarium spp., respectively. Phylogenetic clustering suggested that the amino acid sequences of F1VeA and F4VeA showed high similarity to veA homologs of F. verticillioides and F. fujikuroi deposited in GenBank. Conservative structure domain analysis showed that F1VeA and F4VeA had the sequence characteristics of veA gene family in filamentous fungi, which meant that FocVeA might involve in morphological development, toxin biosynthesis and pathogenicity of F. oxysporum f. sp. cubense.

Key words: Fusarium oxysporum f. sp. Cubense, FoVeA gene, Cloning, Sequence analysis

漆艳香, 张欣, 陆英, 张贺, 蒲金基, 喻群芳, 谢艺贤. 香蕉枯萎病菌全局性调控因子FocVeA基因的克隆及功能预测[J]. 生物技术通报, 2014, 0(10): 101-106.

Qi Yanxiang, Zhang Xin, Lu Ying, Zhang He, Pu Jinji, Yu Qunfan, g Xie Yixian. Cloning and Functional Prediction of the Global Regulator FocVeA Gene from Fusarium oxysporum f. sp. cubense[J]. Biotechnology Bulletin, 2014, 0(10): 101-106.

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