红罗非鱼MyoD基因克隆及序列分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (11): 147-151.

红罗非鱼MyoD基因克隆及序列分析

郭奕惠,范嗣刚,黄桂菊,朱克诚,喻达辉

中国水产科学研究院南海水产研究所农业部南海渔业资源开发利用重点实验室, 广州 510300

收稿日期:2014-04-16 出版日期:2014-11-07 发布日期:2014-11-07
作者简介:郭奕惠,女,助理研究员,研究方向：鱼类功能基因
基金资助:
农业部水生动物遗传育种和养殖生物学重点实验室开放研究基金项目（BM2007-02）,中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金项目（2006A003）

Cloning and Sequence Analysis of MyoD Gene in Red Tilmpa Oreochromis sp.(O.mossambicus×O.niloticus)

Guo Yihui ,Fan Sigang, Huang Guiju, Zhu Kecheng, Yu Dahui

Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture；South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300

Received:2014-04-16 Published:2014-11-07 Online:2014-11-07

摘要/Abstract

摘要： MyoD基因是MRFs家族中的一员,能调控肌肉细胞活性和增殖,在肌肉形成过程中起正调控作用。采用同源基因克隆和RACE方法获得红罗非鱼MyoD基因的cDNA序列。开放阅读框长903 bp,编码300个氨基酸,其中第1-114个氨基酸为碱性结构（Basic domain）,第109-165个氨基酸为螺旋-环-螺旋结构（Helix-Loop-Helix domain）,第199-213个氨基酸为周期蛋白依赖性蛋白激酶4（CDK4）结合区域,第233-249为Helix III结构,无信号肽序列。MyoD蛋白的二级结构为螺旋-环-螺旋二聚体。基于MyoD构建的鱼类系统进化树显示红罗非鱼,尼罗罗非鱼和奥利亚罗非鱼聚为一支。

关键词: MyoD, 序列分析, 克隆, 罗非鱼

Abstract: As a member of myogenic regulatory factors（MRFs）, MyoD play a positive regulation in the process of muscle proliferating.In this study, MyoD cDNA of Red Tilmpa Oreochromis sp.（O.mossambicus×O.niloticus）was obtained using homology cloning strategy and rapid cDNA end amploification. The results showed that the open reading frame（ORF）of MyoD was 903 bp length and encoded 300 amino acids, including basic helix-loop-helix（bHLH）domain which composed of 1^st to 114^th amino acids and 109^th to 165^th amino acids, CDK4 combined structure（199^th to 213^th）and Helix III structure（233^th to 249^th）. The signal peptide was not detected in MyoD. The secondary structure of MyoD protein is HLH dimmer. The phylogenetic trees was constructed based on some fish MyoD ORF and suggested that Oreochromis sp. O. aureaus and O. niloticus were clustered together firstly.

Key words: MyoD , Sequence analysis , Cloning , Red Tilmpa（Oreochromis sp.）

郭奕惠,范嗣刚,黄桂菊,朱克诚,喻达辉. 红罗非鱼MyoD基因克隆及序列分析[J]. 生物技术通报, 2014, 0(11): 147-151.

Guo Yihui ,Fan Sigang, Huang Guiju, Zhu Kecheng, Yu Dahui. Cloning and Sequence Analysis of MyoD Gene in Red Tilmpa Oreochromis sp.(O.mossambicus×O.niloticus)[J]. Biotechnology Bulletin, 2014, 0(11): 147-151.

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