生物技术通报 ›› 2014, Vol. 0 ›› Issue (4): 181-186.

• 研究报告 • 上一篇    下一篇

Fe3+对小球藻的生长及油脂含量的影响

孙远, 刘文彬, 周铁柱, 谢通慧, 梁斌, 张永奎   

  1. (四川大学化学工程学院制药与生物工程系,成都 610065)
  • 收稿日期:2014-02-10 出版日期:2014-04-29 发布日期:2014-04-29
  • 作者简介:孙远,女,硕士研究生,硕士,研究方向:微生物发酵;E-mail:suny88@163.com
  • 基金资助:
    四川大学青年教师科研启动基金项目(2014SC011058)

Effects of Fe3+ on the Growth and Oil Content of Chlorella vulgaris for Biodiesel Production

Sun Yuan, Liu Wenbin, Zhou Tiezhu, Xie Tonghui, Liang Bin, Zhang Yongkui   

  1. (Department of Pharmaceutical & Biological Engineering,School of Chemical Engineering,Sichuan University,Chengdu 610065)
  • Received:2014-02-10 Published:2014-04-29 Online:2014-04-29

摘要: 传统化石能源储量日益减少,生物柴油因其环保可再生性成为优质的石化柴油替代品。利用小球藻生产生物柴油速度快、油脂含量高,受到了广泛关注。为进一步提高小球藻生产生物柴油效率,分别探究了Fe3+的浓度及添加时间对自养和异养小球藻生长及产油的影响,获得最优Fe3+培养条件为:自养小球藻延滞期添加10-3 g/L Fe3+,生物量及油脂含量达2.80g/L及30.90%;异养小球藻指数期添加10-5 g/L Fe3+,生物量及油脂含量达3.30 g/L及29.05%。经脂肪酸分析,以上条件获得的微藻油脂均可作为生物柴油生产原料。

关键词: 小球藻, Fe3+, 自养, 异养, 生长, 油脂

Abstract: Biodiesel is a clean renewable energy as the high quality substitute for traditional diesel. Chlorella vulgaris, used for biodiesel production, has been gaining the attention of the society because of its fast growth and high oil content. Iron ion could improve the growth and oil content of C. vulgaris. Effects of the concentration of Fe3+and the time to add Fe3+on the growth and oil content of autotrophic and heterotrophic C. vulgaris were explored in this study. The best conditions of Fe3+ were as follow:(1)10-3 g/L Fe3+ added in the lag phase of autotrophic C. vulgaris, biomass of 2.80 g/L and oil content of 30.90% were obtained, (2)10-5 g/L Fe3+ added in the exponential phase of heterotrophic C. vulgaris, biomass of 3.30 g/L and oil content of 29.05% were obtained. And the oil produced by C. vulgaris under autotrophic and heterotrophic conditions was an ideal feedstock for biodiesel production.

Key words: Chlorella vulgaris, Fe3+, Autotrophy, Heterotrophy, Growth, Oil content