一株拮抗链霉菌的鉴定及其多相分类特征

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.021

摘要/Abstract

摘要： 通过16S rRNA基因序列分析一株拮抗放线菌JXNU03的系统发育关系，通过生理生化试验分析其生理生化特征，利用菌丝生长率法和杯碟法测定其拮抗活性。结果发现，放线菌JXNU03在高氏一号培养基上培养时，基内菌丝发达，分支多且不断裂，气生菌丝发育良好，孢子丝直形或螺旋状，孢子表面带刺，细胞水解液中检测到葡萄糖，未检测出特征性糖，糖类型属于C型，细胞壁氨基酸中含有L，L-DAP，属于细胞壁Ⅰ型，16S rRNA基因序列与灭癌素链霉菌的同源性高达98%，其发酵液对革兰氏阳性细菌、革兰氏阴性细菌、酵母菌和霉菌等都具有较强的拮抗作用。因此放线菌JXNU03被鉴定为灭癌素链霉菌，记为灭癌素链霉菌JXNU03（Streptomyes gancidicus JXNU03），是一株对细菌、酵母菌和霉菌均有较强拮抗活性的链霉菌，具有潜在开发价值。

关键词: 灭癌素链霉菌, 系统发育, 多相分类

Abstract: To identify a strain of actinomycetes JXNU03 through the sequences of 16S rRNA gene. The physiological characteristics were acquired through physiological and biochemical experiments. The antagonistic activity was determined by plate assay and adopting mycelium growth rate method respectively. When the actinomycetes JXNU03 was cultured on Gause 1 medium, the vegetative hyphae were well developed and abundant without fracture, and the aerial hyphae were grown well with straight or spiral spore hypha. There were some thorns on the surface of spores. The glucose was detected in the whole cell hydrolysis, while not other dominant diagnostic sugars, and the L, L-DAP was detected in cell wall. The phylogenetic analysis showed that the level of 16S rRNA gene sequence similarity between the actinomycete JXNU03 and Streptomyes gancidicus NBRC 15412. The strain JXNU03 demonstrated a board spectrum of antagonistic activity against. The Gram-positive bacteria, the Gram-negative bacteria, yeasts and molds. The actinomycetes JXNU03 was identified as Streptomyes gancidicus, and termed Streptomyes gancidicus JXNU03. As its antagonistic activity against bacteria, yeast and filamentous fungi. The actinomycetes JXNU03 possessed an important prospect for antibiotics development.

Key words: Streptomyes gancidicus, phylogenesis, polyphasic taxonomy

焦林培金,黄运红,李素珍,龙中儿. 一株拮抗链霉菌的鉴定及其多相分类特征[J]. 生物技术通报, 2015, 31(1): 138-143.

Jiao Linpeijin, Huang Yunhong, Li Suzhen, Long Zhonger. Identification and Polyphasic Taxonomy Character of a Strain of Streptomycete[J]. Biotechnology Bulletin, 2015, 31(1): 138-143.

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