生物技术通报 ›› 2015, Vol. 31 ›› Issue (10): 184-190.doi: 10.13560/j.cnki.biotech.bull.1985.2015.10.029

• 研究报告 • 上一篇    下一篇

同步脱氮除硫菌的筛选鉴定及其生长特性研究

黄俊1,张诗颖1,王翻翻1,李月寒1,吴鹏1,宋吟玲1,2   

  1. 1.苏州科技学院环境科学与工程学院, 苏州 215009;
    2.江苏省环境科学与工程重点实验室, 苏州 215009
  • 收稿日期:2014-12-08 出版日期:2015-10-28 发布日期:2015-10-28
  • 作者简介:黄俊, 男, 硕士研究生, 研究方向:水污染控制与理论; E-mail:hjusts@hotmail.com
  • 基金资助:
    江苏省高校自然科学基金项目(13KJB610014), 江苏省环境科学与工程重点实验室开放基金项目(Zd131201),  江苏高校优势学科建设工程资助项目

Screening, Identification and Characterization of Strains Coupling Nitrogen Removal with Sulfide Removal

Huang Jun1, Zhang Shiying1, Wang Fanfan1, Li Yuehan1, Wu Peng1, Song Yinling1, 2   

  1. 1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009; 2. Jiangsu Provincial Key Lab of Environmental Science and Engineering, Suzhou 215009
  • Received:2014-12-08 Published:2015-10-28 Online:2015-10-28

摘要: 为促进反硝化脱硫工艺的工程化应用, 从稳定运行的反硝化脱硫UASB中筛选出2株高活性自养反硝化菌H3和H7, 并对其进行了鉴定和生长及反硝化特性的研究。结果表明, 2株菌均为革兰氏阴性菌, 16S rDNA序列分析表明, 分别与Pseudomonas stutzeri CONC12和 Pseudomonas stutzeri 19smn4相似性为99.6% 和98.9%。结合生理生化特性和16S rDNA序列分析, 确定两菌株都为Pseudomonas stutzeri。对两菌株的生长和反硝化特性研究表明, 菌株H3和H7的生长最适起始pH为6.94和6.88, 最适反硝化pH分别为6.77和6.56。H3和H7的最适生长温度为30.4℃和30.6℃, 最适反硝化温度分别为31.4℃和31.2℃, 两株菌种都为非耐盐菌株。

关键词: 脱氮除硫菌, 16S rDNA, 反硝化特性, 非耐盐菌株

Abstract: In order to promote the engineering application of denitrification and desulfurization process, 2 highly-active autotrophic denitrifying bacterial strains H3 and H7 were screened from stable UASB with denitrification and desulfurization, and the strains were identified and characterized. Two strains were both gram negative bacteria, the 16S rDNA sequence analysis revealed that strain H3 and H7 had a similarity of 99.6%, 98.9% with Pseudomonas stutzeri CONC12 and P. stutzeri 19smn4 respectively. Combing the morphologic, physiobiochemical characteristics and the analysis of its 16S rDNA, 2 strains were identified as P. stutzeri. The results of growth and denitrification of 2 strains demonstrated that the optimal initial pH for H3 and H7 growth were 6.94 and 6.88 respectively, for denitrification were 6.77 and 6.56 respectively. The optimal temperatures for growth were 30.4℃ and 30.6℃, for denitrification were 31.4℃ and 31.2℃ respectively, both of H3 and H7 were non-resistant strains to salt.

Key words: desulfurization denitrifying bacteria, 16S rDNA, denitrification characteristic, non-resistant strain to salt