细菌对重金属吸附和解毒机制的研究进展

摘要/Abstract

摘要： 随着采矿、冶炼和电镀等工业的不断发展，重金属对环境造成的污染也日益严重。近年来提出的微生物修复法，因其成本低和无二次污染等优点而引起国内外学者的广泛关注。目前，用来去除环境中重金属的微生物主要有真菌、藻类、放线菌及细菌等，其中细菌对重金属的微生物吸附和解毒机制研究较为广泛。将从细胞结构的角度，即细菌细胞外部、细胞表面和细胞内部等三方面概述重金属胁迫下细菌的吸附和解毒机制研究进展，主要内容包括细胞外部的沉淀机制、细胞表面的吸附机制以及细胞内部的解毒机制，并提出未来可能的发展方向，旨为重金属污染的微生物修复技术提供理论依据和实践参考。

关键词: 重金属, 细菌, 吸附机制, 解毒机制

Abstract: With the continuous development of mining，smelting and electroplating industries，heavy metals pollutions to the environment are increasingly serious. In recent years，microbial remediation arouses the extensive attentions because of its advantages of low cost and no secondary pollution. The main microorganisms used to remove heavy metals include fungi，algae，actinomyces and bacteria；among them，the biosorption and detoxification mechanisms of bacteria are more widely studied. This paper summaries the research advances on biosorption and detoxification mechanisms of heavy metals by bacteria from the aspects of bacterial cell’s structure，i.e.，the extracellular part，the cell surface and intracellular part，which mainly includes extracellular precipitation mechanisms，biosorption mechanisms on cell surface and intracellular detoxification mechanisms. Furthermore，the paper puts forward the feasible direction of future research，and provides theoretical basis and practical references for the microbial remediation of heavy metals pollution.

Key words: heavy metals, bacteria, biosorption mechanisms, detoxification mechanisms

中图分类号:

10.13560/j.cnki.biotech.bull.1985.2016.12.003

王泽煌,王蒙,蔡昆争，蔡一霞,黄飞,. 细菌对重金属吸附和解毒机制的研究进展[J]. 生物技术通报, 2016, 32(12): 13-18.

WANG Ze-huang,WANG Meng，CAI Kun-zheng，CAI Yi-xia,HUANG Fei,. Research Advances on Biosorption and Detoxification Mechanisms of Heavy Metals by Bacteria[J]. Biotechnology Bulletin, 2016, 32(12): 13-18.

参考文献

[1] 李兆辉, 王光明, 徐云明, 等. 镉、汞、铅污染及其微生物修复研究进展[J] . 中国畜牧兽医, 2010, 37（9）：39-43.
[2] Suranjana AR, Manas KR. Bioremediation of heavy metal toxicity with special reference to chromium[J] . Al Ameen Journal of Medical Sciences, 2009, 2（2）：57-63.
[3] 赵庆龄, 张乃弟, 路文如. 土壤重金属污染研究回顾与展望Ⅱ——基于三大学科的研究热点与前沿分析[J] . 环境科学与技术, 2010, 33（7）：102-106.
[4] Huang F, Dang Z, Guo CL, et al. Biosorption of Cd（II）by live and dead cells of Bacillus cereus RC-1 isolated from cadmium-contaminated soil[J] . Colloids Surf B Biointerface, 2013, 107：11-18.
[5] 王建龙, 陈灿. 生物吸附法去除重金属离子的研究进展[J] . 环境科学学报, 2010, 30（4）：673-701.
[6] Sud D, Mahajan G, Kaur MP. Agricultural waste material as potential adsorbent for sequestering heavy metal ions from aqueous solutions-a review[J] . Bioresour Technol, 2008, 99：6017-6027.
[7] Pavel K, Martina M, Tomas M. Microbial biosorption of metals[M] . Berlin：Springer Science, 2011：320.
[8] Chakraborty J, Das S. Characterization and cadmium-resistant gene expression of biofilm-forming marine bacterium Pseudomonas aerug-inosa JP-11[J] . Environ Sci Pollut Res Int, 2014, 21（24）：14188-14201.
[9] Singh VK, Kumar A. Production and purification of an extracellular cellulase from Bacillus brevis VS-1[J] . Biochem Mol Biol Int, 1998, 45（3）：443-452.
[10] Donati ER, Viera MR, Tavani EL, et al. Sulfidogenesis at low pH by acidophilic bacteria and its potential for the selective recovery of transition metals from mine waters[J] . Advanced Materials Research, 2009, 71-73：693-696.
[11] Fayaz AM, Girilal M, Rahman M, et al. Biosynthesis of silver and gold nanoparticles using thermophilic bacterium Geobacillus stearothermophilus[J] . Process Biocheistry, 2011, 46（10）：1958-1962.
[12] Kambiz AN, Hossein SZ, Sung CY, et al. The production of a cold-induced extracellular biopolymer by Pseudomonas fluorescens BM07 under various growth conditions and its role in heavy metals absorption[J] . Process Biochemistry, 2007, 42：847-855.
[13] Remacle J, Mugaruza I, Fransolet M. Cadmium removal by a strain of Alcaligenes denitrifieam isolated from a metal-polluted pond[J] . Water Resourses, 1992, 26（7）：923-926.
[14] Tripathi MK, Roy U, Jinwal UK, et al. Cloning, sequencing and structural features of a novel Streptococcus lipase[J] . Enzyme and Microbial Technology, 2004, 34：437-445.
[15] Ye JS, Yin H, Peng H, et al. Copper biosorption and ions release by Stenotrophomonas maltophilia in the presence of benzo[a] pyrene[J] . Chemical Engineering Journal, 2013, 219：1-9.
[16] 李辉, 石璐, 张国芳, 等. 蜡状芽孢杆菌SY对镉的吸附机制[J] . 科技导报, 2010, 28（7）：59-62.
[17] 刘瑞霞, 潘建华, 汤鸿霄, 等. Cu（Ⅱ）离子在Micrococcus luteus细菌上的吸附机理[J] . 环境化学, 2002, 1：50-55.
[18] 白洁琼, 尹华, 叶锦绍, 等. 嗜麦芽窄食单胞菌对铜镉的吸附特性与离子交换[J] . 环境科学, 2013（1）：217-225.
[19] Pan XH, Chen Z, Chen YJ, et al. The analysis of the immobilization mechanism of Ni（II）on Bacillus cereus[J] . Journal of Nanoscience and Nanotechnology, 2011, 11（4）：3597-3603.
[20] 刘红娟, 张慧, 党志, 等. 一株耐镉细菌的分离及其富集Cd的机理[J] . 环境工程学报, 2009, 3（2）：367-371.
[21] Bahari ZM, Altowayti W, Ibrahim Z, et al. Biosorption of As（III）by Non-living biomass of an arsenic-hypertolerant Bacillus cereus strain SZ2 isolated from a gold mining environment：equilibrium and kinetic study[J] . Appl Biochemi Biotechnol, 2013, 8：2247.
[22] 杨洲平, 陈平, 王振宇, 等. 粗毛栓菌和蜡状芽孢杆菌及其共固定菌对Pb（Ⅱ）的吸附[J] . 应用生态学报, 2012, 23（8）：2212-2218.
[23] Joo J, Hassan SHA, Oh S. Comparative study of biosorption of Zn2+ by Pseudomonas aeruginosa and Bacillus cereus[J] . International Biodeterioration and Biodegradation, 2010, 64（8）：734-741.
[24] Bai J, Yang XH, Du RY, et al. Biosorption mechanisms involved in immobilization of soil Pb by Bacillus subtilis DBM in a multi-metal-contaminated soil[J] . J Eeviron Sci, 2014, 10：2056-2064.
[25] Valenzuela C, Campos V, Yanez J, et al. Isolation of arsenite-oxidizing bacteria from arsenic-enriched sediments from Camarones River, Northern Chile[J] . Bulletin of Environmental Contamination and Toxicology, 2009, 82（5）：593-596.
[26] 陈亚刚, 陈雪梅, 张玉刚, 等. 微生物抗重金属的生理机制[J] . 生物技术通报, 2009（10）：60-65.
[27] Fan H, Su C, Wang Y, et al. Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China[J] . Journal of Applied Microbiology, 2005, 105（2）：529-539.
[28] Li L, Hu Q, Zeng JH, et al. Resistance and biosorption mechanism of silver ions by Bacillus cereus biomass[J] . Journal of Environmental Sciences, 2011, 23（1）：108-111.
[29] Shan Q, Fang M, Huang X, et al . Study on the adsorption of bacteria in ceramsite and their synergetic effect on adsorption of heavy metals[J] . Water Sci Technol, 2014, 69（2）：407-413.
[30] 刘义, 何钢, 陈介南, 等. 细菌的锌抗性基因及其在生物修复中的应用[J] . 生物技术通报, 2008, 2：35-38.
[31] Baldi F, Gallo M, et al. Seasonal mercury transformation and surficial sediment detoxification by bacteria of Marano and Grado lagoons[J] . Estuari Coast Shelf Sci, 2012, 113：105-115.
[32] Osman D, Waldron KJ, Denton H, et al. Copper homeostasis in Salmonella is atypical and copper-CueP is a major periplasmic metal complex[J] . J Biol Chem, 2010, 33：25259-25268.
[33] Wang XY, Chen MLi, et al. Genome sequence analysis of the naph-thenic acid degrading and metal resistant bacterium Cupriavidus gilardii CR3[J] . PLoS One, 2015, 8：e0132881.
[34] 李樊, 刘义, 孙伟峰, 等, 铜绿假单胞菌czcCBA基因与生物修复[J] . 生物技术通报, 2010（11）：44-47.
[35] Boutoille D, Jacqueline C, Le Mabecque V, et al. In vivo impact of the MexAB-OprM efflux system on beta-lactam efficacy in an experimental model of Pseudomonas aeruginosa infection[J] . Int J Antimicrob Agents, 2009, 33（5）：417-420.
[36] Stahl A, Pletze D, Mehmood A, et al. Marinobacter adhaerens HP15 harbors two CzcCBA efflux pumps involved in zinc detoxification[J] . Antonie Van Leeuwenhoek, 2015, 108：649-658.
[37] Andres LT, Lorena NA, et al. CtpA, a putative Mycobacterium tube-rculosis P-type ATPase, is stimulated by copper（I）in the mycob-acterial plasma membrane[J] . Biometals, 2015, 28：713-724.
[38] Zhang WW, Yin K, Li BW, et al. A glutathione S-transferase from Proteus mirabilis involved in heavy metal resistance and its potential application in removal of Hg2+[J] . Journal of Hazardous Materials, 2013, 261：646-652.
[39] 王伟. 恶臭假单胞菌CD2 P型ATP酶基因cadA1抗Zn2+功能的确定[D] . 武汉：华中农业大学, 2008.
[40] Giovanella P, Costa AP, Schffer N, et al. Detoxification of mercury by bacteria using crude glycerol from biodiesel as a carbon source[J] . Water Air Soil Pollut, 2015, 226：224.
[41] Santos-Gandelman JF, Cruz K, Crane S, et al. Potential application in mercury bioremediation of a marine sponge-Isolated Bacillus cereus strain Pj1[J] . Curr Microbiol, 2014, 69（3）：374-380.
[42] Rahman Z, Singh VP. Cr（VI）reduction by Enterobacter sp. DU17 isolated from the tannery waste dump site and characterization of the bacterium and the Cr（VI）reductase[J] . International Biodeterioration and Biodegradation, 2014, 91：97-103.
[43] Yamamura S, Amachi S. Microbiology of inorganic arsenic：From metabolism to bioremediation[J] . Journal of Bioscience and Bioengineering, 2014, 118（1）：1-9.
[44] Bahar MM, Megharaj M, Naidu R. Oxidation of arsenite to arsenate in growth medium and groundwater using a novel arsenite-oxidizing diazotrophic bacterium isolated from soil[J] . International Biodeterioration and Biodegradation, 2016, 106：178-182.
[45] Li J, Wang Q, Zhang SZ, et al. Phylogenetic and genome analyses of antimony-oxidizing bacteria isolated from antimony mined soil[J] . Int Biodeterior Biodegradation, 2013, 76：76-80.
[46] Sinha A, Kumar S, Khare SK. Biochemical basis of mercury remediation and bioaccumulation by Enterobacter sp. EMB21[J] . Appl Biochem Biotechnol, 2013, 169（1）：256-267.
[47] Vasak M. Advances in metallothionein structure and functions. [J] . J Trace Elem Med Biol, 2005, 19（1）：13-17.
[48] Ward SK, Abomoelak B, Hoye EA, et al. CtpV：a putative copper exporter required for full virulence of Mycobacterium tuberculosis[J] . Mol Microbiol, 2010, 77（5）：1096-1110.