土壤酸杆菌门细菌生态学研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.002

摘要/Abstract

摘要： 酸杆菌是土壤中一类重要的细菌类群。基于16S rRNA基因序列分析发现，酸杆菌一般占细菌总量的20%左右，甚至高达50%以上，表明酸杆菌在土壤生态过程中起到重要的作用。从植被、海拔高度、氮肥管理及二氧化碳升高对土壤酸杆菌分布的影响，以及酸杆菌根际效应等几方面论述了土壤酸杆菌门细菌生态学研究进展；综合分析揭示出不同分类单元酸杆菌细菌分布与环境因子间的关系；阐述了部分酸杆菌细菌的潜在生态功能。最后指出在土壤酸杆菌研究中应强化分离培养、细化分子生态、采用宏基因组学和单细胞测序新技术的重要性。

关键词: 酸杆菌, 微生物生态, 亚群, 16S rRNA基因, 高通量测序

Abstract: The phylum Acidobacteria is one of the most important bacterial groups in soils.Based on analysis of 16S rRNA gene sequences, the number of Acidobacteria generally represents about 20% of total soil bacterial communities, some of them even account for more than 50%, which suggests that Acidobacteria play important roles in soil ecological process.In this paper, we reviewed the research progress of Acidobacteria ecology in soils from several aspects, such as effects of plant types, altitude, nitrogen fertilizer and CO₂ enrichment on the distribution of Acidobacteria, as well as rhizosphere effects of Acidobacteria, etc.The relationships between distributions of different taxonomic level Acidobacteria and environmental factors were summarized.The potential ecological functions of Acidobacteria were also presented in this paper.In the end of this paper, the importance for future study of Acidobacteria, such as reinforcement of isolation and pure culture, refinement of molecular ecological research, and adoption of metagenomics and single-cell sequencing approaches were also addressed.

Key words: Acidobacteria, microbial ecology, subdivision, 16S rRNA gene, high-throughput sequencing

王光华, 刘俊杰, 于镇华, 王新珍, 金剑, 刘晓冰. 土壤酸杆菌门细菌生态学研究进展[J]. 生物技术通报, 2016, 32(2): 14-20.

WANG Guang-hua, LIU Jun-jie, YU Zhen-hua, WANG Xin-zhen, JIN Jian, LIU Xiao-bing. Research Progress of Acidobacteria Ecology in Soils[J]. Biotechnology Bulletin, 2016, 32(2): 14-20.

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