不同紫细菌光合基因表达调控机制异同

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.005

摘要/Abstract

摘要： 紫细菌是一类可以进行不放氧光合作用的原核微生物，可以利用光能产生ATP并为其生长代谢提供能量。其光系统由一系列色素及蛋白组成的复合体组成，并由一系列光合基因如puc、puf、bch和crt等编码。紫细菌光合相关基因的表达主要受到外界氧化还原信号及光照的影响，然而不同紫细菌光合基因表达调控机制具有明显的多样性。以球形红细菌与沼泽红假单胞菌为重点，介绍了近年来几种光合基因表达调控系统如PpsR/CrtJ型调控蛋白、双组分磷酸化调控系统、CRP-FNR型调控蛋白等在紫细菌中的研究进展。通过系统深入分析这些调控通路在不同紫细菌中的特征及功能，发现不同菌株中类似调控通路通常具有相似功能，但又各具特点。旨为对进一步了解紫细菌光合基因表达调控机制并为其应用研究提供参考。

关键词: 光合基因, 球形红细菌, 沼泽红假单胞菌, 紫细菌

Abstract: The purple bacteria are prokaryotes that can proceed photosynthesis without oxygen release.They use light energy to produce ATP for their own growth and metabolism.The photosystem of a purple bacterium consists of a series of complex of pigments and proteins encoded by photosynthetic genes like puc, puf, puh, bch, crt and so on.The expressions of their photosynthetic genes mainly are influenced by light and external redox signals；however, the expression and regulation mode of photosynthetic genes are greatly different between different kinds of purple bacteria.This review introduces the research advances on several expression and regulation systems of photosynthetic genes, such as PpsR/CrtJ like regulator, two-component phosphorylation regulation system and CRP-FNR like regulator in purple bacteria while focusing on Rhodobacter sphaeroides and Rhodopseudomonas palustris.The results from the functional and characteristic analysis of the regulation mechanism in different purple bacteria suggest that, each regulation system existing in different bacterial strain shows similar functions but with somewhat unique characteristics.This study gives us further insights for understanding the mechanisms of expression and regulation of photosynthetic genes in the purple bacteria, and provides important references for application of these purple bacteria in the future.

Key words: photosynthetic genes, Rhodobacter sphaeroides, Rhodopseudomonas palustris, purple bacteria

邓春浩, 孙良昱, 陈坤明. 不同紫细菌光合基因表达调控机制异同[J]. 生物技术通报, 2016, 32(2): 21-29.

DENG Chun-hao, SUN Liang-yu, CHEN Kun-ming. Differences in Regulation of Photosynthetic Gene Expression Between Different Purple Bacteria[J]. Biotechnology Bulletin, 2016, 32(2): 21-29.

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