萜类化合物的合成生物学研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017.01.007

摘要/Abstract

摘要： 萜类化合物是种类最多的一类天然产物，具有抗癌、抗过敏等多种生物活性，在食品、日化、医疗等领域受到广泛关注，展现了巨大的应用潜力和广阔的市场前景。近年来，研究人员采用功能基因组学和代谢组学技术对不同萜类的合成途径进行了深入研究，为萜类的合成生物学研究提供了大量的数据支撑。目前，已经通过合成生物学方法构建出萜类高产的酵母工程菌株，实现了多种目标产物的高效生产，有效提高了萜类的总体生产水平。因此，采用合成生物学策略合成萜类化合物，有望成为植物源萜类生产的有效技术手段。首先介绍了合成生物学概念，进而总结了植物源萜类的重要功能和应用领域，并简述了不同萜类的合成途径，归纳了现有的萜类生产方式，然后深入探讨了萜类生物合成的设计策略，最后以几种常见的萜类为例，详细论述了不同萜类的合成生物学的研究进展。

关键词: 类化合物, 生物合成, 萜类合成设计, 合成生物学进展

Abstract: The terpenoids represent the largest class of natural products with biological activities of antitumor and anti-allergy,thus they have been widely applied in the area of food,cosmetics and medical health,presenting huge potential and broad market prospects. Recent years,researchers applied functional genomics and metabonomics approaches to deeply study the biosynthesis pathways of terpenoids,providing tons of data for their synthetic biology. The construction of engineered yeasts using synthetic biology enabled the efficient synthesis of multi-target terpenoids,and highly improved the overall production level. Thus,the synthetic biology approach is expected to be an efficient way of producing plant-derived terpenoids. First,we introduced the concept of synthetic biology,summarized the important functions and applications of plant-derived terpenoids,briefly reviewed the biosynthesis pathways,and concluded the alternative production ways. Then,we discussed the design strategies of synthetic biology for terpenoids thoroughly. Finally,we elaborated the advances on the biosynthetic biology of varied terpenes with common terpenes as the studied cases.

Key words: terpenoids, biosynthesis, terpene synthesis and design, progresses on synthetic biology

孙丽超，李淑英，王凤忠，辛凤姣. 萜类化合物的合成生物学研究进展[J]. 生物技术通报, 2017, 33(1): 64-75.

SUN Li-chao, LI Shu-ying, WANG Feng-zhong, XIN Feng-jiao. Research Progresses in the Synthetic Biology of Terpenoids[J]. Biotechnology Bulletin, 2017, 33(1): 64-75.

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