水稻Bph14基因生理功能初探

doi:10.13560/j.cnki.biotech.bull.1985.2017-0754

摘要/Abstract

摘要： Bph14 基因是第一个被克隆的水稻抗虫基因，但鲜有Bph14基因对水稻生理方面影响的报道。研究该基因对水稻生理方面的影响有助于全面理解Bph14基因对褐飞虱的抗性机制。将Bph14基因转入水稻栽培品种中，对携带有外源基因的转基因株系通过荧光定量PCR检测乙烯合成和非生物胁迫相关基因的表达，另外，分别用5 μmol/L ABA和250 mmol/L NaCl处理转基因水稻株系和相应受体材料，结果表明，与乙烯合成相关的基因OsACO2，OsACS2，OsACS6，O1gP5CS 和 O5gP5CS 的表达量均较转基因受体m5274发生了变化，与干旱、耐盐等非生物胁迫相关的基因CatA、CatB、CatC、RAB16A、LEA3、LIP9、SalT、AdhI等也都不同于在受体m5274中的表达。同时Bph14基因的导入也提高了水稻对ABA的敏感性和对盐胁迫的耐受性，说明Bph14基因在水稻中可能参与调控乙烯合成以及非生物胁迫应答的功能基因的表达。

关键词: Bph14基因, 乙烯合成, 非生物胁迫, 水稻

Abstract: Gene Bph14 is the first cloned rice insect-resistant gene，however，there were few reports about the effects of Bph14 gene on rice physiology. The study of the physiological effects of the gene on rice would contributes to a comprehensive understanding of the resistance mechanism of gene Bph14 to brown planthopper. The gene Bph14 was transferred into rice cultivar m5274，and genes related to ethylene biosynthesis and abiotic stress response were detected by Q-PCR in transgenic lines carrying gene Bph14. In addition，transgenic lines and the accepter line were treated with 5 μmol/L ABA and 250 mmol/L NaCl respectively. In these lines，OsACO2，OsACS2，OsACS6，O1gP5CS，and O5gP5CS expressed differently from those in m5274. Genes such as CatA，CatB，CatC，RAB16A，LEA3，LIP9，SalT，and AdhI related to abiotic stress of drought and salt-resistance also differently expressed from those in m5274. At the same time，the introduction of Bph14 gene improved the sensitivity of rice to ABA and the resistance to salt stress. These results indicate that gene Bph14 in rice probably participates in the regulation of ethylene biosynthesis and expression of functional genes in abiotic stress response.

Key words: Bph14 gene, ethylene biosynthesis, abiotic stress, rice

李三和，闸雯俊, 陈志军，周雷, 刘凯，游艾青，. 水稻Bph14基因生理功能初探[J]. 生物技术通报, 2017, 33(12): 93-98.

LI San-he, ZHA Wen-jun, CHEN Zhi-jun, ZHOU Lei, LIU Kai, YOU Ai-qing. Preliminary Study on Physiological Function of Gene Bph14 in Rice[J]. Biotechnology Bulletin, 2017, 33(12): 93-98.

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