关键词: 沙门氏菌, 耐药基因, 耐药性

Abstract: The aim of this study is to analyze the relationship between resistance genes of Salmonella in swine and antimicrobial resistance. The antimicrobial resistances of 60 Salmonella strains from swine to 10 drugs were determined by K-B method，and the resistance genes of Salmonella were detected by PCR. The results showed that the antimicrobial resistance of the isolated Salmonella to ciprofloxacin was the highest（98.3% of them were resistant），and about 75% of the strains were resistant to sulfamethoxazole trimethoprim and amikacin. They were more sensitive to gentamicin，tochloramphenicol，norfloxacin，and spectinomycin，and the rate was over 45%. The results of the resistance gene test demonstrated that the detection rates of 7 resistance genes tetA，parC，gyrA，sulI，sulII，floR，and aadA1 were 53.3%，98.3%，66.7%，46.7%，33.3%，48.3%，and 66.3%，respectively，while the resistance gene tetB，tetC，tetG，cat1 and Aaca（3）-Ia were not even detected. Furthermore，by cloning and analyzing the several common resistance genes in the successfully isolates，it is concluded that the resistance genes of Salmonella may determine the resistance phenotype of a bacterium.

Key words: Salmonella, resistance genes, antimicrobial resistance