真菌DWL-C010的鉴定及其可溶性红色素热稳定性分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0129

摘要/Abstract

摘要： 从广西大王岭自然保护区内原始森林土壤中分离得到一株能够产可溶性红色素真菌DWL-C010,通过形态特征和多基因位点系统发育分析对其进行了鉴定,并分析了该菌红色素的热稳定性,同时还测定了其纤维素半纤维酶和糖化酶酶活。结果表明,菌株DWL-C010形态特征与白二轮蓝状菌基本一致,且其ITS、RBP2、RBP1和BenA序列与白二轮篮状菌CBS133440T相应序列的一致性均高达99%,CaM序列一致性为96%,并且在系统发育树中二者均聚在了一起,故将其鉴定为白二轮篮状菌。菌株DWL-C010红色素具有较好的热稳定性,其不同温度下的热解常数与时间具有良好的线性关系,符合一级动力学模型;依赖温度的热降解常数也符合Arrhenius模型,其活化能为51.82 kJ·mol-1。此外,菌株DWL-C010第6天的木聚糖酶、纤维素内切酶及糖化酶酶活依次为33.098、1.880和0.976 U/mL。

关键词: 红色素, 白二轮蓝状菌, 鉴定, 热稳定

Abstract: Strain DWL-C010 capable of secreting diffusible red pigment,isolated from primary forest humus soil of Dawangling Nature Reserve in Gxuangxi,was identified by morphological characteristics and phylogenetic analysis of multiple gene loci,and then the thermal stability of its red pigment was analyzed as well. In addition,the enzyme activity of its cellulase,hemicellulase,and glucoamylase was also evaluated. The results showed that morphological characteristics of strain DWL-C010 are consistent with Talaromyces albobiverticillius on the whole,and the identity of ITS RBP2,RBP1 and BenA between strain DWL-C010 and T. albobiverticillius CBS133440T reach 99%,while the identity of CaM has only 96%;but they all resolve in a clade with T. albobiverticillius CBS133440T. Referring to the traditional morphological observation,strain DWL-C010 belongs to be T. albobiverticillius. At the same time,the thermal stability of red pigment from DWL-C010 is promising,the degradation rate of red pigment from strain DWL-C010 was in fine linear relation with time,and it could be well described by a first order model. The temperature-dependent degradation constants also followed the Arrhenius model,and the activation energy of the red pigment is 51.82 kJ/mol. Besides,the enzyme activity of strain DWL-C010 for xylanase,cellulase and glucoamylase was 33.098,1.880 and 0.976 U/mL,respectively.

Key words: red pigment, Talaromyces albobiverticillius, identification, thermal stability

马博, 张婷婷, 吴宝祥. 真菌DWL-C010的鉴定及其可溶性红色素热稳定性分析[J]. 生物技术通报, 2018, 34(4): 186-193.

MA Bo, ZHANG Ting-ting, WU Bao-xiang. Identification of Filamentous Fungus Strain DWL-C010 and Thermal Stability Analysis of Its Soluble Pigment[J]. Biotechnology Bulletin, 2018, 34(4): 186-193.

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