H2O2对黑曲霉氧化胁迫机理的研究

doi:10.13560/j.cnki.biotech.bull.1985.2018-0026

摘要/Abstract

摘要： 旨在探究H₂O₂胁迫下,黑曲霉的氧化应激响应,为控制黑曲霉生长和赭曲霉毒素A（OTA）合成提供依据。测定黑曲霉菌体生长、OTA合成、胞内活性氧（ROS）和脂质过氧化水平,以及抗氧化酶活性。H₂O₂能够抑制黑曲霉生长,且抑制作用具有剂量相关性;能促进其合成OTA,尤其是生长第4天时最为明显;能提升胞内ROS水平和丙二醛（MDA）含量;引起胞内过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性增高,其中在生长第5天CAT酶活提高显著,生长第6 天 GPX酶活提高显著;但对超氧化物歧化酶（SOD）活性无显著影响。菌体通过提升胞内抗氧化酶活性及毒素的合成来平衡胞内过多的活性氧,从而在氧胁迫下维持菌体生长及代谢。

关键词: 黑曲霉, 赭曲霉毒素A, 活性氧, 抗氧化酶, 氧化胁迫

Abstract: To investigate the response of Aspergillus niger to oxidative stress by adding H₂O₂ to the medium will provide the basis for controlling the mycelial growth and ochratoxin A（OTA）biosynthesis of A. niger. The effects of H₂O₂ on the growth,OTA biosynthesis,intracellular reactive oxygen species（ROS）formation,lipid peroxidation level,and antioxidase activities in A. niger were investigated. H₂O₂ inhibited the mycelial growth of A. niger,and the inhibition was dose dependent. Also H₂O₂ promoted OTA biosynthesis in A. niger,especially the most remarkably at the day 4 of growth. H₂O₂ caused intracellular ROS and malondialdehyde（MDA）content increasing in A. niger. H₂O₂ enhanced the activities of intracellular catalase（CAT）and glutathione peroxidase（GPX）,while significantly at the day 5 of growth for CAT and at the day 6 for GPX in A. niger. However,H₂O₂ presented no significant effect on the activity of superoxide dismutase（SOD）in A. niger. A. niger remediates oxidative stress by enhancing the activity of antioxidant enzymes and producing OTA,which may maintain the growth and metabolism of A. niger under oxygen stress.

Key words: Aspergillus niger, ochratoxin A, reactive oxygen species, antioxidase, oxidative stress

陈浩宇, 徐瑞涛, 程志翔, 高强, 张健. H₂O₂对黑曲霉氧化胁迫机理的研究[J]. 生物技术通报, 2018, 34(4): 201-207.

CHEN Hao-yu, XU Rui-tao, CHENG Zhi-xiang, GAO Qiang, ZHANG Jian. Responses of Asperigillus niger to Oxidative Stress Under H₂O₂ Exposure[J]. Biotechnology Bulletin, 2018, 34(4): 201-207.

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H₂O₂对黑曲霉氧化胁迫机理的研究

Responses of Asperigillus niger to Oxidative Stress Under H₂O₂ Exposure

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