海洋生物共附生真菌的分离、鉴定及抗菌活性分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0856

生物技术通报 ›› 2019, Vol. 35 ›› Issue (3): 59-64.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0856

海洋生物共附生真菌的分离、鉴定及抗菌活性分析

张圣良¹, 楚肖肖¹, 赵友兴², 孔凡栋², 黄小龙¹

1. 海南大学热带农林学院,海口 570100;
2. 中国热带农业科学院热带生物技术研究所,海口 570100

收稿日期:2018-09-30 出版日期:2019-03-26 发布日期:2019-04-03
作者简介:张圣良,男,硕士研究生,研究方向：海洋微生物;E-mail：1258591541@qq.com
基金资助:
海南省高等学校教育教学改革研究项目（Hnjg2017ZD-3）,海南大学教育教学研究项目（hdjy1703）

Isolation,Identification,and Antibacterial Activity of Fungi Associated with Marine Organisms

ZHANG Sheng-liang¹, CHU Xiao-xiao¹, ZHAO You-xing², KONG Fan-dong², HUANG Xiao-long¹

1. Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570100;
2. Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 570100

Received:2018-09-30 Published:2019-03-26 Online:2019-04-03

摘要/Abstract

摘要： 对海南文昌海域采集的海草、海绵和石珊瑚的共附生真菌进行分离、鉴定及抗菌活性分析。采用3种分离培养基分离海草、海绵和石珊瑚的共附生真菌,利用ITS序列分析对分离的真菌进行鉴定,采用滤纸片扩散法对分离真菌的发酵产物进行抗菌活性分析。结果从10种海草、9种海绵和11种石珊瑚中共获得113株真菌。ITS序列分析初步确定了32株独立菌株的分类地位,归属于3个门（子囊菌门、担子菌门和半知菌门）,8个目和10个属,其中子囊菌门所占比例最高为58.82%,半知菌门次之为32.35%,担子菌门最少为8.83%;抗菌活性测试显示12株真菌的发酵产物对枯草芽孢杆菌和金黄色葡萄球菌具有较强的抗菌活性。初步揭示了海南文昌海域海草、海绵和石珊瑚的共附生真菌的多样性以及其发酵产物的抗菌活性。

关键词: 海草, 海绵, 石珊瑚, 分离, 抗菌活性

Abstract: Isolation,identification and antibacterial activity analysis of fungus associated with sea grasses,sponges and stony corals from Wenchang sea area of Hainan were conducted. Three media were used to isolate and purify the fungi associated with these marine organisms,the isolated fungi were identified according to their ITS sequences,and their antibacterial activities were tested using standard disk diffusion. Total 113 strains of fungi were isolated from 10 sea grasses,9 sponges,and 11 stony corals. The 32 strains of independent fungi,belonging to 10 genera within 8 orders of Ascomycota,Basidiomycotina and Deutemycotina,were identified by ITS sequences analysis. The percentage of Ascomycota was as high as 58.82%,that of Deutemycotina was 32.35% and Basidiomycotina was 8.83%,respectively. The results of antibacterial activity showed that the fermentation products of 12 strains of fungi had strong antibacterial activity against Bacillus subtilis and Staphylococcus aureus. In sum,the diversity and antibacterial activity of fungi associated with sea grasses,sponges and stony corals from Wenchang sea area of Hainan are revealed initially.

Key words: sea grasses, sponges, stony corals, isolation, antibacterial activity

张圣良, 楚肖肖, 赵友兴, 孔凡栋, 黄小龙. 海洋生物共附生真菌的分离、鉴定及抗菌活性分析[J]. 生物技术通报, 2019, 35(3): 59-64.

ZHANG Sheng-liang, CHU Xiao-xiao, ZHAO You-xing, KONG Fan-dong, HUANG Xiao-long. Isolation,Identification,and Antibacterial Activity of Fungi Associated with Marine Organisms[J]. Biotechnology Bulletin, 2019, 35(3): 59-64.

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海洋生物共附生真菌的分离、鉴定及抗菌活性分析

Isolation,Identification,and Antibacterial Activity of Fungi Associated with Marine Organisms

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