一种耐盐促生菌筛选、鉴定及对玉米幼苗生长的影响

doi:10.13560/j.cnki.biotech.bull.1985.2019-0145

生物技术通报 ›› 2019, Vol. 35 ›› Issue (8): 27-33.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0145

一种耐盐促生菌筛选、鉴定及对玉米幼苗生长的影响

孙培¹, 王罡¹, 张亚楠¹, 李倩¹, 季静¹, 杨丹¹, 袁东¹, 王畅¹, 王昱蓉², 王萍²

1. 天津大学环境科学与工程学院,天津 300000;
2. 天津市天大天福生物技术有限公司,天津 300000

收稿日期:2019-02-25 出版日期:2019-08-26 发布日期:2019-08-05
作者简介:孙培,男,硕士研究生,研究方向：环境微生物;E-mail：sunpeitju@163.com
基金资助:
转基因检测和基因改造及其在植物和微生物中的利用（2017GKF-0294）

Screening and Identification of a Salt-tolerant Growth-promoting Bacterium and Its Effect on the Growth of Maize Seedlings

SUN Pei¹, WANG Gang¹, ZHANG Ya-nan¹, LI Qian¹, JI Jing¹, YANG Dan¹, YUAN Dong¹, , WANG Chang¹, WANG Yu-rong², WANG Ping²

1. School of Environmental Science and Engineering,Tianjin University,Tianjin 300000;
2. Tianjin Tianda Tianfu Biotechnology Limited,Tianjin 300000

Received:2019-02-25 Published:2019-08-26 Online:2019-08-05

摘要/Abstract

摘要： 采用10%盐浓度的LB培养基从玉米根际筛选并分离出一株能够耐高盐的细菌菌株,利用形态特征和16S rDNA序列对该菌进行鉴定并分析其促生特性,通过盆栽试验研究在盐胁迫下,该菌株对玉米幼苗生长的影响。研究结果表明,该菌为嗜根考克氏菌Y1,具有溶磷性;在正常条件下,将嗜根考克氏菌Y1定殖在玉米幼苗根际上,株高和根长变化量分别增加了44.96%和49.45%左右,总叶绿素含量提高了74.44%左右,而MDA的含量降低了0.65倍左右;在盐胁迫下,株高和根长变化量分别提高了1.2倍和1.17倍左右,总叶绿素含量提高了56.24%左右,CAT活性提高了0.9倍左右,而MDA含量降低了0.34倍左右。结果表明,嗜根考克氏菌Y1可以作为生物肥料应用于盐渍化土壤中,缓解盐对玉米幼苗生长的抑制,促进玉米幼苗的生长。

关键词: 根际促生菌, 溶磷性, 促生性, 盐渍化

Abstract: A strain of salt-tolerant bacteria was screened and isolated from maize rhizosphere using 10% salt concentration LB medium. The strain was identified by morphological characteristics and 16S rDNA sequence,and its growth-promoting characteristics were analyzed. The effect of the strain on maize seedling growth under salt stress was studied by pot experiment. The results showed that the strain was Kocuria rhizophila Y1 with phosphorus solubility. Under normal conditions,when K. rhizophila Y1 was colonized in the rhizosphere of maize seedlings,the plant height and root length increased by 44.96% and 49.45%,the total chlorophyll content increased by 74.44%,respectively,while the MDA content decreased by 0.65 times. Under salt stress,plant height and root length increased by 1.2 and 1.17 times,total chlorophyll content increased by 56.24%,CAT activity increased by 0.9 times,respectively,while MDA content decreased by 0.34 times. Therefore,K. rhizophila Y1 can be used as a biological fertilizer in saline soil to alleviate the inhibition of salt to the growth of maize seedlings and subsequently to promote the growth of maize seedlings.

Key words: rhizosphere growth promoting bacteria, phosphorus solubility, growth promoting, salinization

孙培, 王罡, 张亚楠, 李倩, 季静, 杨丹, 袁东, 王畅, 王昱蓉, 王萍. 一种耐盐促生菌筛选、鉴定及对玉米幼苗生长的影响[J]. 生物技术通报, 2019, 35(8): 27-33.

SUN Pei, WANG Gang, ZHANG Ya-nan, LI Qian, JI Jing, YANG Dan, YUAN Dong, , WANG Chang, WANG Yu-rong, WANG Ping. Screening and Identification of a Salt-tolerant Growth-promoting Bacterium and Its Effect on the Growth of Maize Seedlings[J]. Biotechnology Bulletin, 2019, 35(8): 27-33.

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一种耐盐促生菌筛选、鉴定及对玉米幼苗生长的影响

Screening and Identification of a Salt-tolerant Growth-promoting Bacterium and Its Effect on the Growth of Maize Seedlings

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