橡胶草SRPP2基因克隆及表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2019-0684

摘要/Abstract

摘要： TkSRPP2是橡胶草SRPP基因家族的成员,在天然橡胶生物合成和橡胶粒子稳定方面起重要作用。采用PCR技术从橡胶草中克隆得到TkSRPP2基因全长序列,对其进行生物信息学分析、烟草亚细胞定位及定量表达分析。结果显示,TkSRPP2基因全长633 bp,编码210个氨基酸,蛋白质相对分子质量为23.15 kD,理论等电点（pI）8.51。系统进化分析表明TkSRPP2蛋白与莴苣SRPP3蛋白亲缘关系最近。烟草亚细胞定位显示TkSRPP2蛋白位于细胞质中。TkSRPP2基因在主要在根中表达,在6月龄橡胶草根部表达量最高。

关键词: 橡胶草, 小橡胶粒子蛋白（SRPP）, 生物信息学分析, 时空表达分析, 亚细胞定位

Abstract: TkSRPP2 is a member of SRPP family in Taraxacum kok-saghyz Rodin,and plays an important role in the biosynthetics of natural rubber and stability of rubber particles. PCR was applied to clone the full length of TkSRPP2 gene from T. kok-saghyz Rodin,then the bioinformatics analysis of TkSRPP2 gene,subcellular localization and quantitative expression analysis of tobacco were conducted. The results demonstrated that the full length of TkSRPP2 gene was 633 bp,encoding 210 amino acids,the relative molecular weight of the protein was 23.15 kD,and the theoretical isoelectric point（pI）was 8.51. The phylogenetic analysis demonstrated that it was most closely related to SRPP3.The subcellular localization of tobacco revealed that TkSRPP2 protein was in cytoplasm. TkSRPP2 gene was expressed in many tissues of T. kok-saghyz Rodin,with the highest expression in 6 months roots.

Key words: Taraxacum kok-saghyz Rodin, Small rubber particle protein（SRPP）, Bioinformatics analysis, Real-time quantitative PCR, Protein Subcellular Localization

张睿珠, 江羽宸, 黄俊, 闫洁. 橡胶草SRPP2基因克隆及表达分析[J]. 生物技术通报, 2020, 36(1): 9-14.

ZHANG Rui-zhu, JIANG Yu-chen, HUANG Jun, YAN Jie. Cloning and Expression Analysis of SRPP2 Gene in Taraxacum kok-saghyz Rodin[J]. Biotechnology Bulletin, 2020, 36(1): 9-14.

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