奶牛分娩后早期血浆代谢物变化研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-1065

摘要/Abstract

摘要： 旨在研究分娩及产后早期奶牛血浆代谢物的变化规律。选取胎次、预产期和体况相近健康高产荷斯坦奶牛15头。分别在分娩当天和产后第7天晨饲前尾静脉采集血液10 mL。采用高效液相色谱-四极飞行时间质谱联用技术（UHPLC-QTOF-MS）对奶牛的血浆进行了分析。结合主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）;以P<0.05、VIP>1和FC>1.3或FC<0.77作为标准筛选两组血浆差异代谢物;ROC曲线进一步研究了这些显著差异代谢物分类识别的能力。结果显示：经ROC曲线发现11个显著性上调差异代谢物：磷脂酰胆碱（16∶0/16∶0）、二醇胆酸、脯氨酸-丝氨酸、脯氨酸-甘氨酸、1-硬脂酰基-sn-甘油3-磷酸胆碱、乳清、L-天门冬氨酸、N-乙酰基-L-天冬氨酸、异戊酰甘氨酸、L-肉碱和苯甲酸。丙氨酸,天冬氨酸和谷氨酸代谢、甘油磷脂代谢和嘧啶代谢产后第7天与分娩当天代谢轮廓存在显著差异。综上表明：产后血浆中N-乙酰基-L-天冬氨酸、L-天门冬氨酸、磷脂酰胆碱和乳清的升高有利维持奶牛机体健康。

关键词: 产后奶牛, 分娩, 代谢物

Abstract: This work aims to study the changes of plasma metabolites in dairy cows in the calving and postpartum period. Fifteen healthy and high-yield dairy cows with similar parity,expected date of birth and similar body conditions were selected for collecting 10 mL blood from the tail vein on the day of calving and on the 7^th day after calving when before morning feeding. The blood plasma of the cows was analyzed by ultra-high performance liquid chromatography-quadrupole-time-of-fight-mass spectrometry（UHPLC-QTOF-MS）. Combined with principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）,plasma differential metabolites between 2 groups were screened based on the P<0.05,VIP>1 and FC>1.3 or FC<0.77. The ROC curve further was used to study the ability of these significant differential metabolites to be classified and identified. The results showed that 11 significant up-regulated differential metabolites were found by ROC curve：PC（16：0/16：0）,glycolithocholic acid,pro-ser,pro-gly,L-stearoyl-sn-glycerol 3-phosphocholine,orotic acid,L-aspartate,N-acetyl-L-aspartic acid,isovalerylglycine,L-calving-carnitine and benzoic acid. There were significant differences in the metabolic profiles of alanine,aspartic acid and glutamic acid metabolism,glycerophospholipid metabolism and pyrimidine metabolism between the day of calving and on the 7^th day after calving. In summary,the changes of N-acetyl-L-aspartic acid,L-aspartic acid,PC（16：0/16：0）and orotic acid are beneficial to maintain the health of cows after postpartum period.

Key words: postpartum dairy cows, calving, metabolite

张萌, 罗芳, 王敏, 武彦泽, 王俊奎, 和东迁, 陈丽尧, 陶金忠. 奶牛分娩后早期血浆代谢物变化研究[J]. 生物技术通报, 2020, 36(6): 191-199.

ZHANG Meng, LUO Fang, WANG Min, WU Yan-ze, WANG Jun-kui, HE Dong-qian, CHEN Li-yao, TAO Jin-zhong. Changes in Plasma Metabolites After Calving in Dairy Cows[J]. Biotechnology Bulletin, 2020, 36(6): 191-199.

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