围产前期添加山楂和黄芪混合物对奶牛血浆代谢组的影响

doi:10.13560/j.cnki.biotech.bull.1985.2019-0300

摘要/Abstract

摘要： 旨为研究日粮中添加山楂、黄芪对产前奶牛血浆代谢物的影响。选取年龄、胎次和体况相近围产期健康高产荷斯坦奶牛20头。随机分为2组,对照组A组（n=10）：饲喂基础日粮;试验组B组（n=10）：饲喂基础日粮添加复方中草药山楂和黄芪（各150 g）。进入围产圈开始饲喂,连续饲喂14 d,晨饲前尾静脉采血10 mL,采用高效液相色谱-质谱（HPLC-MS）技术,结合主成分分析（PCA）和正交偏最小二乘法-判别分析（OPLS-DA）对两组血浆代谢轮廓进行模式识别;以VIP>1、P<0.05和FC>1.3或FC<0.77作为标准筛选两组血浆差异代谢物;ROC曲线进一步确定这些差异代谢物对两组血浆分类的识别能力。结果显示：添加山楂和黄芪对D-谷氨酰胺和D-谷氨酸代谢、亚油酸代谢、牛磺酸和亚牛磺酸代谢、丙氨酸、天冬氨酸和谷氨酸代谢、缬氨酸、亮氨酸和异亮氨酸的生物合成有显著的影响,经ROC曲线发现9个显著性上调差异代谢物：肌酐、5,2'-O-甲基胞苷、肉豆蔻酸、亮氨酸、烟酸、3-羟基苯甲酸、次黄嘌呤、γ-谷氨酰缬氨酸和马尿酸。综上表明：产前饲料添加山楂、黄芪影响奶牛的氨基酸代谢,脂肪代谢和糖代谢。

关键词: 围产期奶牛, 山楂, 黄芪, 代谢物

Abstract: This work aims to study the effects of adding hawthorn and astragalus on the plasma metabolites of prenatal dairy cows. Twenty healthy and high-yielding dairy cows with similar age,parity and body condition were selected,and then randomly divided into 2 groups,i.e.,control group A（n=10）：fed basal diet;test group B（n=10）：supplemented with compound Chinese herbal hawthorn and astragalus（150 g per cow）. Feeding for consecutive 14 days while the perinatal circle started,collecting 10 ml blood from the front tail vein before morning feeding,high performance liquid chromatography-mass spectrometry（HPLC-MS）combined with principal component analysis（PCA）and orthogonal partial least squares-Discriminant analysis（OPLS-DA）were used for pattern recognition of plasma metabolic profiles in both groups. Plasma differential metabolites between 2 groups were screened based on the VIP>1,P<0.05,and FC>1.3 or FC<0.77. The ROC curve was employed to further determine the recognition ability of these differential metabolites to plasma classification in 2 groups. The results showed that there was significant difference in the metabolism profile of D-glutamine and D-glutamic acid,linoleic acid,taurine and hypotaurine,alanine,aspartic acid and glutamic acid,ammonia,leucine and isoleucine between the test group and the control group. Nine significant up-regulated differential metabolites were identified by ROC curve：creatinine,5,2'-O-methylcytidine,myristoleic acid,L-leucine,nicotinate,3-hydroxybenzoic acid,hypoxanthine,γ-glutamyl proline and hippuric acid. In summary,the addition of hawthorn and astragalus to prenatal feed affects the amino acid metabolism,fat metabolism and glucose metabolism of dairy cows.

Key words: perinatal dairy cow, hawthorn, astragalus, metabolite

张萌, 刘国林, 李向龙, 陈永宏, 白玲荣, 罗芳, 李亚超, 陶金忠. 围产前期添加山楂和黄芪混合物对奶牛血浆代谢组的影响[J]. 生物技术通报, 2019, 35(8): 127-137.

ZHANG Meng, LIU Guo-lin, LI Xiang-long, CHEN Yong-hong, BAI Ling-rong, LUO Fang, LI Ya-chao, TAO Jin-zhong. Effect of Adding Hawthorn and Astragalus Mixtures on the Plasma Metabolome of Perinatal Dairy Cows[J]. Biotechnology Bulletin, 2019, 35(8): 127-137.

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