马铃薯根际产IAA芽孢杆菌的分离鉴定及促生效果研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-1203

摘要/Abstract

摘要： 从黑龙江地区马铃薯根际土壤中分离得到11株具有较好的产IAA能力的芽孢杆菌,其中对产量较高的HL379进行形态学、Biolog以及16S rDNA序列分析鉴定其种属,采用拟南芥促生实验以及马铃薯盆栽实验进行促生功能研究。经鉴定该菌株为阿耶波多氏芽孢杆菌（Bacillus aryabhattai）,IAA产量为47.8 mg/L,经盆栽实验发现,马铃薯株高、鲜重、干重、根长等分别增加了13%、32%、107%、20.8%,马铃薯产量增加38%,对马铃薯的生长具有显著的促进作用。说明HL379在植物促生方面具有一定的应用潜力。

关键词: 马铃薯, 吲哚乙酸, 促生功能研究, 植物根际细菌

Abstract: Eleven Bacillus strains that have promising ability of producing IAA were isolated from the potato rhizosphere soil in Heilongjiang. The strain HL379 with high yield was identified by morphology,Biolog and 16S rDNA sequence analysis. The pot experiments of growing Arabidopsis thaliana and potato were carried out to study its growth promotion function. The strain was identified as Bacillus aryabhattai,and the yield of IAA was 47.8 mg/L. Growth experiments in the pot showed that potato plant height,fresh weight,dry weight,and root length increased by 13%,32%,107% and 20.8%,respectively,and potato yield increased by 38%,meaning that it had a significant promotion effect on potato growth. It reveals that HL379 has certain application potential in plant growth promotion.

Key words: potato, indole acetic acid, growth promotion function, plant rhizosphere bacteria

李培根, 要雅倩, 宋吉祥, 王天琪, 周波, 王冰, 林榕姗. 马铃薯根际产IAA芽孢杆菌的分离鉴定及促生效果研究[J]. 生物技术通报, 2020, 36(9): 109-116.

LI Pei-gen, YAO Ya-qian, SONG Ji-xiang, WANG Tian-qi, ZHOU Bo, WANG Bing, LIN Rong-shan. Isolation and Identification of IAA-producing Bacillus sp on Potato Rhizosphere and Its Growth-promoting Effect[J]. Biotechnology Bulletin, 2020, 36(9): 109-116.

参考文献

[1] 王颖慧, 蒙美莲, 陈有君, 等. 覆膜方式对旱作马铃薯产量和土壤水分的影响[J]. 中国农学通报, 2013(3):147-152.
Wang YH, Meng ML, Chen YJ, et al.Effect of different film-covering modes on the yield and soil moisture of dry land tillage potato[J]. Chinese Agricultural Science Bulletin, 2013(3):147-152.
[2] 杨雅伦, 郭燕枝, 孙君茂. 我国马铃薯产业发展现状及未来展望[J]. 中国农业科技导报, 2017, 19(1):29-36.
Yang YL, Guo YZ, Sun JM.Present status and future prospect for potato industry in China[J]. Journal of Agricultural Science and Technology, 2017, 19(1):29-36.
[3] Bais HP, Weir TL, Perry LG, et al.The role of root exudates in rhizosphere interactions with plants and other organisms[J]. Annual Review of Plant Biology, 2006, 57(1):233-266.
[4] Phillips KA, Skirpan AL, et al.Vanishing tassel2 encodes a grass-specific tryptophan aminotransferase required for vegetative and reproductive development in maize[J]. Plant Cell, 2011, 23(2):550-566.
[5] Woodward JB, Abeydeera ND, Paul D, et al.A maize thiamine auxotroph is defective in shoot meristem maintenance[J]. Plant Cell, 2010, 22(10):3305-3317.
[6] 张东艳, 刘晔, 吴越, 等. 花生根际产IAA菌的筛选鉴定及其效应研究[J]. 中国油料作物学报, 2016, 38(1):104-110.
Zhang DY, Liu Y, Wu Y, et al.Isolation and identification of IAA-producing strains from peanut rhizosphere and its promoting effects on peanut growth[J]. Chinese Journal of Oil Crop Sciences, 2016, 38(1):104-110.
[7] Youry P, Tanja M, Nicola T, et al.Microbial interactions in the rhizosphere:beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. A review[J]. Biology and Fertility of Soils, 2015, 51(4):403-415.
[8] Fahad S, Hussain S, Bano A, et al.Potential role of phytohormones and plant growth-promoting rhizobacteria in abiotic stresses:consequences for changing environment[J]. Environmental Science and Pollution Research, 2015, 22(7):4907-4921.
[9] Beneduzi A, Ambrosini A, Passaglia LM, et al.Plant growth-promoting rhizobacteria(PGPR):Their potential as antagonists and biocontrol agents[J]. Genetics and Molecular Biology, 2012, 35(Suppl4):1044-1051.
[10] Zamioudis C, Korteland J, Van Pelt JA, et al.Rhizobacterial volatiles and photosynthesis-related signals coordinate MYB72 expression in Arabidopsis roots during onset of induced systemic resistance and iron-deficiency responses[J]. Plant J, 2015, 84(2):309-322.
[11] 葛春辉, 孟阿静, 马彦茹, 等. 一株黄瓜根际促生菌的筛选、鉴定及其发酵特性[J]. 生物技术通报, 2014(3):94-99.
Ge CH, Meng AJ, Ma YR, et al.Selection, identification and characteristics of a cucumber growth-promotion strain of rhizobacteria[J]. Biotechnology Bulletin, 2014(3):94-99.
[12] Patten CL, Glick BR.Bacterial biosynthesis of indole-3-acetic acid[J]. Canadian Journal of Microbiology, 1996, 42(3):207-220.
[13] Tien TM, Gaskins MH, Hubbell DH.Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet(Pennisetum americanum L.)[J]. Applied and Environmental Microbiology, 1979, 37(5):1016-1024.
[14] Jiang Y, Wu Y, Xu WS, et al.IAA-producing bacteria and bacterial-feeding nematodes promote arabidopsis thaliana root growth in natural soil[J]. Europ J Soil Biol, 2012, 52:22-26.
[15] Shim J, Kim JW, Shea PJ, et al.IAA production by Bacillus sp. JH 2-2 promotes Indian mustard growth in the presence of hexavalent chromium.[J]. Journal of Basic Microbiology, 2015, 55(5):652-658.
[16] 吕泽勋, 宋未. 培养条件对产酸克雷伯氏菌SG-11生物合成IAA影响的研究[J]. 应用与环境生物学报, 2000(1):66-69.
Lv ZX, Song W.Effect of cultural conditions on IAA biosynthesis by klebsiella oxytoca SG-11[J]. Chinese Journal of Applied & Environmental Biology, 2000(1):66-69.
[17] 杨帆, 孟远夺, 姜义, 等. 2013年我国种植业化肥施用状况分析[J]. 植物营养与肥料学报, 2015, 21(1):217-225.
Yang F, Meng YD, Jiang Y, et al.Chemical fertilizer application and supply in crop farming in China in 2013[J]. Plant Nutrition and Fertilizer Science, 2015, 21(1):217-225.
[18] 张海亮, 王智博. 西北某地区农村农药化肥污染问题的调查研究[J]. 中国集体经济, 2016(33):16-17.
Zhang HL, Wang ZB.Investigation on the pollution of pesticides and fertilizers in a rural area Northwest China[J]. Collective Economy, 2016(33):16-17.
[19] Fan XH, Zhang SA, Mo XD, et al.Effects of plant growth-promoting rhizobacteria and N source on plant growth and N and P uptake by tomato grown on calcareous soils[J]. Pedosphere, 2017, 27(6):1027-1036.
[20] 万兵兵, 刘晔, 吴越, 等. 一株玉米根际多功能促生菌的筛选鉴定及效应研究[J]. 生物技术通报, 2016, 32(8):169-176.
Wan BB, Liu Y, Wu Y, et al.Screening and identification of maize growth-promoting rhizobacteria and its promoting effects on maize[J]. Biotechnology Bulletin, 2016, 32(8):169-176.
[21] 徐婧, 邵锴, 李东芳, 等. 产IAA菌的筛选、鉴定及其培养基的优化[J]. 苏州科技大学学报:自然科学版, 2018, 35(2):45-54.
Xu J, Shao K, Li DF.Selection, identification of an IAA biosynthesis strain and the optimization of its culture medium[J]. Journal of Suzhou University of Science and Technology:Natural Science, 2018, 35(2):45-54.
[22] 田宏先, 崔林, 王秀英, 等. 马铃薯内生促生菌的促生长作用[J]. 山西农业科学, 2003(1):28-30.
Tian HX, Cui L, Wang XY, et al.Growth promoting activity of endophytic bacterial isolated from potato[J]. Journal of Shanxi Agricultural Sciences, 2003(1):28-30.
[23] Kesaulya H, Baharuddin, Zakaria B, et al.Isolation and physiolog-ical characterization of PGPR from potato plant rhizosphere in medium land of Buru Island[J]. Procedia Food Science, 2015, 3:190-199.
[24] 梁允刚, 孟晶, 谭兰玉, 等. 马铃薯晚疫病生防菌的分离鉴定与防治效果[J]. 北方农业学报, 2017, 45(4):54-57.
Liang YG, Meng J, Tan LY, et al.Isolation and identification of biocontrol strain and its effect on the control of potato late blight[J]. Inner Mongolia Agricultural Science and Technology, 2017, 45(4):54-57.
[25] 高同国, 姜军坡, 郭晓军, 等. 马铃薯疮痂病高效拮抗菌的筛选及鉴定[J]. 江苏农业科学, 2016, 44(12):157-159.
Gao TG, Jiang JP, Guo XJ, et al.Screening and identification of antagonistic bacteria against Potato Scab[J]. Jiangsu Agricultural Sciences, 2016, 44(12):157-159.
[26] 宋本超, 赵冬梅, 杨志辉, 等. 马铃薯黑痣病菌拮抗菌的筛选鉴定及生防因子分析[J]. 生物技术通报, 2019, 35(8):9-16.
Song BC, Zhao DM, Yang ZH.et al.Screening and identification of an antagonistic bacterium against Rhizoctonia solani and analysis of biocontrol factor[J]. Biotechnology Bulletin, 2019, 35(8):9-16.
[27] Shivaji S, Chaturvedi P, Begum Z, et al.Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere[J]. Int J Syst Evol Microbiol, 2009, 59(12):2977-2986.
[28] Wen J, Ren C, Huang N, et al.Draft genome of bagasse-degrading bacteria Bacillus aryabhattai GZ03 from deep sea water[J]. Marine Genomics, 2015, 19:13-14.
[29] Yan Y, Zhang L, Yu MY, et al.The genome of Bacillus aryabhattai T61 reveals its adaptation to Tibetan Plateau environment[J]. Genes & Genomics, 2016, 38(3):293-301.
[30] Paredes-Páliz KI, Caviedes MA, Doukkali B, et al.Screening beneficial rhizobacteria from Spartina maritima for phytoremedia-tion of metal polluted salt marshes:comparison of gram-positive and gram-negative strains[J]. Environmental Science and Pollution Research, 2016, 23(19):19825-19837.
[31] Pan F, Yu Y, Xu A, et al.Application of magnetic OMS-2 in sequencing batch reactor for treating dye wastewater as a modulator of microbial community[J]. Journal of Hazardous Materials, 2017, 340:36-46.
[32] Lee S, Ka JO, Song HG.Growth promotion of Xanthium italicum by application of rhizobacterial isolates of Bacillus aryabhattaiin microcosm soil[J]. Journal of Microbiology, 2012, 50(1):45-49.
[33] 杨莹, 刘冬雪, 郭英, 等. 2株芽孢杆菌抗旱及解磷能力[J]. 江苏农业科学, 2019, 47(4):260-263.
Yang Y, Liu DX, Guo Y, et al.Drought resistance and phosphate solubilizing ability of two Bacillus Strains[J]. Jiangsu Agricultural Sciences, 2019, 47(4):260-263.
[34] 刘军生, 解修超, 罗阳兰, 等. 抗镉内生细菌阿耶波多氏芽孢杆菌的分离鉴定及生物学特性[J]. 生物技术通报, 2019, 35(2):64-72.
Liu JS, Xie XC, Luo YL, et al.Isolation, identification, and biological characteristics of a cadmium-resistant endophytic bacteria Bacillus aryabhattai[J]. Biotechnology Bulletin, 2019, 35(2):64-72.