碱胁迫条件下含ACC脱氨酶的根际细菌对大豆生长的影响

doi:10.13560/j.cnki.biotech.bull.1985.2020-0936

摘要/Abstract

摘要： 碱胁迫导致作物严重减产。接种含ACC脱氨酶的根际细菌能提高植物对多种非生物胁迫的抵御能力,但是关于这类细菌能否提高植物耐碱性的研究却寥寥无几。本实验以含ACC脱氨酶的根际细菌V. paradoxus 5C-2和大豆中黄13为实验材料,探究碱胁迫条件下,接菌对大豆生长状况的影响。研究结果表明40 mmol/L NaCl处理对大豆生长没有影响,但是含40 mmol/L Na⁺的碱性盐（pH值分别为8.12和9.04）处理16 d后,大豆地上部和根系的生物量、侧根总长度、叶绿素含量以及光合效率均明显降低,气孔阻力显著升高。接菌处理后,在每一种处理条件下,大豆地上部和根系的干重均有所增加。尤其在pH值为9.04的条件下,接菌处理后大豆的侧根总长度、叶绿素含量以及最大光合潜能均明显升高,气孔阻力显著下降。这些实验结果表明在高pH值条件下接种根际细菌V.paradoxus 5C-2能缓解碱性盐对大豆的胁迫效应。

关键词: 碱胁迫, ACC脱氨酶, 大豆, 根际促生细菌

Abstract: Alkaline stress leads to severe crop loss. Inoculation of ACC-deaminase-containing rhizobacteria can improve plant resistance to several abiotic stresses,however,little research has been done on whether such bacteria can improve plant resistance to alkalinity. Using the ACC deaminase-containing rhizobacteria Variovorax paradoxus 5C-2 and Glycine max（L.）Merr. Huang 13 as the experimental materials,we studied the effects of the inoculation of V. paradoxus 5C-2 on the growth of soybean under alkali stress. The results showed that 40 mmol/L NaCl treatment presented no effect on soybean growth,but at 16 d after treated with alkaline salts（pH values 8.12 and 9.04,respectively）containing 40 mmol/L Na⁺,the biomass of soybean aboveground and root,total lateral root length,chlorophyll content and photosynthetic efficiency dramatically reduced,while stomatal resistance significantly increased. After inoculation of the rhizobacteria,the dry weight of shoot and root increased. Under the condition of pH 9.04,the total lateral root length,chlorophyll content and maximum photosynthetic potential of soybean increased clearly after the inoculation,and stomatal resistance decreased significantly. These experimental results demonstrated that,under the high pH condition,inoculation of rhizospheric bacteria V. paradoxus 5C-2 may alleviate the stress effects of alkaline salts on soybean plant.

Key words: alkaline stress, ACC deaminase, soybean, growth-promoting rhizobacteria

梁烨, 何楚婷, 杨悦, 张玉芬, 姜帆. 碱胁迫条件下含ACC脱氨酶的根际细菌对大豆生长的影响[J]. 生物技术通报, 2020, 36(9): 100-108.

LIANG Ye, HE Chu-ting, YANG Yue, ZHANG Yu-fen, JIANG Fan. Effects of Inoculation of Rhizobacteria Containing ACC Deaminase on Soybean Growth Under Alkaline Stress[J]. Biotechnology Bulletin, 2020, 36(9): 100-108.

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