白灵侧耳野生种质群体生长发育相关性状评价

doi:10.13560/j.cnki.biotech.bull.1985.2021-1147

摘要/Abstract

摘要：

通过对野生种质群体生长发育性状的表型鉴定和分析,揭示我国特有种类白灵侧耳种质资源的多样性和可利用性。从国家标准菌株库的库藏中随机选择了100个白灵侧耳野生菌株,对其生长发育性状,即PDA培养基平板中的菌丝生长速度、棉籽壳培养料试管中的菌丝长速及原基形成时间等3个性状进行了表型测定和分析。根据性状数据的统计分析发现3个性状均为多基因控制的数量性状,且性状间不具有显著的相关性;菌丝长速性状具有较高的广义遗传力,说明性状的遗传变异主要由基因型决定。来源于不同地区的菌株原基形成所需的时间存在显著性差异,发现来自于托里县的菌株原基形成所需的时间最短,而来自于青河县的菌株原基形成时间最长,推测可能由不同来源地菌株的进化机制不同造成。研究结果为发掘白灵侧耳育种的可利用种质提供了科学依据,为将优异种质资源转化为可利用的亲本材料提供理论基础。

关键词: 白灵侧耳, 野生种质, 菌丝长速, 原基形成, 表型评价

Abstract:

The phenotypic identification and analysis of growth and development traits for the wild germplasm of Pleurotus tuoliensis were conducted,thereby the diversity and the utilizability of the germplasm from the special P. tuoliensis were explored. A total of 100 wild strains were randomly selected from the collection of China Center for Mushroom Spawn Standards and Control（CCMSSC）. The mycelial growth rate on PDA plates and that in substrate tubes of cottonseed husk as culture material,as well as the formation time of primordia were tested and analyzed. According to the results of statistics,the three tested traits were all quantitative traits controlled by multiple loci,and there was no significant correlation among the three tested traits. The broad-sense heritability of the mycelial growth rates was high,indicating that the phenotypic variation of traits was mainly determined by genotypes although influenced by environments. Primordium formation time of strain group from different geographical origin varied significantly,the shortest for the strain from Tuoli,and the longest for the strains from Qinghe,suggesting that it resulted from the different evolution mechanism for different-origin strains. The findings of this study provides scientific basis for exploring useful germplasm in breeding P. tuoliensis and theoretical foundation for converting excellent germplasm into useful parent material.

Key words: Pleurotus tuoliensis, wild germplasm, mycelial growth rate, primordia formation, phenotypic evaluation

杨雅舒, 汪颖, 高巍. 白灵侧耳野生种质群体生长发育相关性状评价[J]. 生物技术通报, 2021, 37(11): 101-108.

YANG Ya-shu, WANG Ying, GAO Wei. Evaluation of the Growth and Development Traits for Wild Germplasm of Pleurotus tuoliensis[J]. Biotechnology Bulletin, 2021, 37(11): 101-108.

图/表 9

参考文献 19

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菌株编号 Strain code	采集地点 Collection location	菌株编号 Strain code	采集地点 Collection location	菌株编号 Strain code	采集地点 Collection location	菌株编号 Strain code	采集地点 Collection location
CCMSSC00930	青河	CCMSSC03141	青河	CCMSSC02498	裕民	CCMSSC02681	裕民
CCMSSC00933	青河	CCMSSC03165	青河	CCMSSC02502	裕民	CCMSSC02687	裕民
CCMSSC00938	青河	CCMSSC03656	青河	CCMSSC02506	裕民	CCMSSC02692	裕民
CCMSSC00947	青河	CCMSSC03668	青河	CCMSSC02507	裕民	CCMSSC02694	裕民
CCMSSC00956	青河	CCMSSC03683	青河	CCMSSC02511	裕民	CCMSSC02698	裕民
CCMSSC00973	青河	CCMSSC03689	青河	CCMSSC02514	裕民	CCMSSC02700	裕民
CCMSSC02995	青河	CCMSSC03205	托里	CCMSSC02579	裕民	CCMSSC02703	裕民
CCMSSC03001	青河	CCMSSC03207	托里	CCMSSC02590	裕民	CCMSSC02704	裕民
CCMSSC03003	青河	CCMSSC03209	托里	CCMSSC02596	裕民	CCMSSC02706	裕民
CCMSSC03006	青河	CCMSSC03211	托里	CCMSSC02597	裕民	CCMSSC02708	裕民
CCMSSC03010	青河	CCMSSC03216	托里	CCMSSC02599	裕民	CCMSSC02710	裕民
CCMSSC03048	青河	CCMSSC03219	托里	CCMSSC02610	裕民	CCMSSC02719	裕民
CCMSSC03058	青河	CCMSSC03235	托里	CCMSSC02611	裕民	CCMSSC02726	裕民
CCMSSC03064	青河	CCMSSC03245	托里	CCMSSC02632	裕民	CCMSSC02729	裕民
CCMSSC03065	青河	CCMSSC03293	托里	CCMSSC02634	裕民	CCMSSC02742	裕民
CCMSSC03070	青河	CCMSSC03585	托里	CCMSSC02635	裕民	CCMSSC02747	裕民
CCMSSC03073	青河	CCMSSC02242	裕民	CCMSSC02644	裕民	CCMSSC02748	裕民
CCMSSC03086	青河	CCMSSC02247	裕民	CCMSSC02660	裕民	CCMSSC02787	裕民
CCMSSC03108	青河	CCMSSC02250	裕民	CCMSSC02667	裕民	CCMSSC02789	裕民
CCMSSC03112	青河	CCMSSC02253	裕民	CCMSSC02668	裕民	CCMSSC02796	裕民
CCMSSC03118	青河	CCMSSC02382	裕民	CCMSSC02670	裕民	CCMSSC02798	裕民
CCMSSC03127	青河	CCMSSC02485	裕民	CCMSSC02671	裕民	CCMSSC03201	裕民
CCMSSC03129	青河	CCMSSC02494	裕民	CCMSSC02672	裕民	CCMSSC03553	裕民
CCMSSC03133	青河	CCMSSC02495	裕民	CCMSSC02677	裕民	CCMSSC03555	裕民
CCMSSC03139	青河	CCMSSC02497	裕民	CCMSSC02680	裕民	CCMSSC03568	裕民

菌株编号 Strain code	采集地点 Collection location	菌株编号 Strain code	采集地点 Collection location	菌株编号 Strain code	采集地点 Collection location	菌株编号 Strain code	采集地点 Collection location
CCMSSC00930	青河	CCMSSC03141	青河	CCMSSC02498	裕民	CCMSSC02681	裕民
CCMSSC00933	青河	CCMSSC03165	青河	CCMSSC02502	裕民	CCMSSC02687	裕民
CCMSSC00938	青河	CCMSSC03656	青河	CCMSSC02506	裕民	CCMSSC02692	裕民
CCMSSC00947	青河	CCMSSC03668	青河	CCMSSC02507	裕民	CCMSSC02694	裕民
CCMSSC00956	青河	CCMSSC03683	青河	CCMSSC02511	裕民	CCMSSC02698	裕民
CCMSSC00973	青河	CCMSSC03689	青河	CCMSSC02514	裕民	CCMSSC02700	裕民
CCMSSC02995	青河	CCMSSC03205	托里	CCMSSC02579	裕民	CCMSSC02703	裕民
CCMSSC03001	青河	CCMSSC03207	托里	CCMSSC02590	裕民	CCMSSC02704	裕民
CCMSSC03003	青河	CCMSSC03209	托里	CCMSSC02596	裕民	CCMSSC02706	裕民
CCMSSC03006	青河	CCMSSC03211	托里	CCMSSC02597	裕民	CCMSSC02708	裕民
CCMSSC03010	青河	CCMSSC03216	托里	CCMSSC02599	裕民	CCMSSC02710	裕民
CCMSSC03048	青河	CCMSSC03219	托里	CCMSSC02610	裕民	CCMSSC02719	裕民
CCMSSC03058	青河	CCMSSC03235	托里	CCMSSC02611	裕民	CCMSSC02726	裕民
CCMSSC03064	青河	CCMSSC03245	托里	CCMSSC02632	裕民	CCMSSC02729	裕民
CCMSSC03065	青河	CCMSSC03293	托里	CCMSSC02634	裕民	CCMSSC02742	裕民
CCMSSC03070	青河	CCMSSC03585	托里	CCMSSC02635	裕民	CCMSSC02747	裕民
CCMSSC03073	青河	CCMSSC02242	裕民	CCMSSC02644	裕民	CCMSSC02748	裕民
CCMSSC03086	青河	CCMSSC02247	裕民	CCMSSC02660	裕民	CCMSSC02787	裕民
CCMSSC03108	青河	CCMSSC02250	裕民	CCMSSC02667	裕民	CCMSSC02789	裕民
CCMSSC03112	青河	CCMSSC02253	裕民	CCMSSC02668	裕民	CCMSSC02796	裕民
CCMSSC03118	青河	CCMSSC02382	裕民	CCMSSC02670	裕民	CCMSSC02798	裕民
CCMSSC03127	青河	CCMSSC02485	裕民	CCMSSC02671	裕民	CCMSSC03201	裕民
CCMSSC03129	青河	CCMSSC02494	裕民	CCMSSC02672	裕民	CCMSSC03553	裕民
CCMSSC03133	青河	CCMSSC02495	裕民	CCMSSC02677	裕民	CCMSSC03555	裕民
CCMSSC03139	青河	CCMSSC02497	裕民	CCMSSC02680	裕民	CCMSSC03568	裕民

性状Traits	最小值 Min	最大值 Max	均值 Mean	标准差 Standard deviation	变异系数 Coefficient of variation	广义遗传力 Generalized heritability
PDA-平板上菌丝长速 Mycelial growth rate on the PDA- plate/（mm·d^-1）	3.26	10.64	6.46	2.00	13.60	0.97
培养料试管中菌丝长速 Mycelial growth rate in test tube/（mm·d^-1）	1.93	4.66	2.76	0.43	11.50	0.90
原基形成时间 Primordium formation time/（d）	23.00	130.00	45.14

性状Traits	最小值 Min	最大值 Max	均值 Mean	标准差 Standard deviation	变异系数 Coefficient of variation	广义遗传力 Generalized heritability
PDA-平板上菌丝长速 Mycelial growth rate on the PDA- plate/（mm·d^-1）	3.26	10.64	6.46	2.00	13.60	0.97
培养料试管中菌丝长速 Mycelial growth rate in test tube/（mm·d^-1）	1.93	4.66	2.76	0.43	11.50	0.90
原基形成时间 Primordium formation time/（d）	23.00	130.00	45.14

变异来源Source of variation	自由度df	平方和Sum of squares	均方Mean square	方差Variance	显著性P
菌株编号Strain	99	1441.974	14.5654	18.87	<0.001
测量次数Number of measurements	1	141.134	141.134	182.82	<0.001
菌株和测量次数Strains and number of measurements	99	375.5633	3.7936	4.91	<0.001
残差Residual	361	278.692	0.772
总数Total	560	2237.363