生物技术通报 ›› 2022, Vol. 38 ›› Issue (11): 175-184.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0353
王刚1(), 罗建勋2, 蒲尚饶3, 李亚平1, 王刚1(), 孙志鹏2()
收稿日期:
2022-03-25
出版日期:
2022-11-26
发布日期:
2022-12-01
作者简介:
王刚,男,博士研究生,研究方向:药用植物栽培生理;E-mail:基金资助:
WANG Gang1(), LUO Jian-xun2, PU Shang-rao3, LI Ya-ping1, WANG Gang1(), SUN Zhi-peng2()
Received:
2022-03-25
Published:
2022-11-26
Online:
2022-12-01
摘要:
光照是植物生长的必要环境因子,揭示不同遮荫处理对云曼红豆杉苗木活性成分10-DAB、矿质营养累积和光合作用的影响,为云曼红豆杉林下综合利用和林-草复合生态系统的构建提供科学依据。以2年生云曼红豆杉实生苗为材料,设置5种光强条件,阐明云曼红豆杉对不同光照环境的响应策略。结果表明,遮荫对云曼红豆杉苗木各生长时期10-DAB累积具有显著影响(P < 0.05),10-DAB产量在4-10月呈上升趋势,10月达到最大值;随着遮荫的增强,各部位矿质元素的累积量均呈先增加再降低的趋势,其中,N和P在75%遮荫处理下最高,含量大小依次为叶>根>茎,K在50%遮荫处理下最高,含量大小依次为叶>茎>根;遮荫可以显著提高云曼红豆杉光合色素含量(P < 0.05),随遮荫的增强呈“先升后降”的变化规律,75%遮荫下最大,净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)的变化规律同光合色素相一致,胞间CO2浓度(Ci)则是先降低后增加,在95%遮荫下的各指标(除Ci)均最低。重度遮荫抑制了云曼红豆杉光合作用,遮荫强度可以有效提高云曼红豆杉苗木(枝叶)10-DAB产量及矿质营养累积,云曼红豆杉苗木培育宜选50%-75%遮荫。
王刚, 罗建勋, 蒲尚饶, 李亚平, 王刚, 孙志鹏. 遮荫对云曼红豆杉活性成分10-DAB及矿质营养累积的影响[J]. 生物技术通报, 2022, 38(11): 175-184.
WANG Gang, LUO Jian-xun, PU Shang-rao, LI Ya-ping, WANG Gang, SUN Zhi-peng. Effects of Shading on the Active Component 10-DAB and Mineral Nutrient Accumulation of Taxus madia × T. Yunnanensis ‘Yunman’[J]. Biotechnology Bulletin, 2022, 38(11): 175-184.
图1 不同遮荫处理云曼红豆杉苗木10-DAB含量、枝叶生物量 不同小写字母表示各遮荫处理间差异显著(P<0.05)。下同
Fig. 1 10-DAB content and branch and leaf biomasses of YM(Taxus madia × T. Yunnanensis ‘Yunman’)seedlings in different shading treatments Different lowercase letters indicate significant differences among shading treatments(P<0.05). The same below
图5 云曼红豆杉苗木根(a)、茎(b)和叶(c)中N、P、K含量及其化学计量比间的相关性分析 * 表示P<0.05,** 表示P<0.01
Fig. 5 Correlation analysis of N,P and K contents and th-eir stoichiometric ratios in the roots(a),stems(b)and leaves(c)of young YM seedlings * indicate P<0.05, ** indicate P<0.01
遮荫处理Shading treatment | 叶绿素a Chl a/(mg·g-1) | 叶绿素b Chl b/(mg·g-1) | 总叶绿素Total Chl/(mg·g-1) | 类胡萝卜素Carotenoids/(mg·g-1) |
---|---|---|---|---|
CK | 0.818±0.047b | 0.627±0.034b | 1.433±0.065b | 0.563±0.029b |
30% | 0.957±0.059b | 0.823±0.120a | 1.787±0.237a | 0.712±0.084a |
50% | 1.372±0.120a | 0.835±0.129a | 2.206±0.349a | 0.841±0.149a |
75% | 1.532±0.112a | 0.853±0.061a | 2.315±0.231a | 0.865±0.053a |
95% | 0.728±0.041b | 0.535±0.037b | 1.262±0.071b | 0.496±0.053b |
表1 不同遮荫处理对云曼红豆杉苗木叶绿素和类胡萝卜素含量的影响
Table 1 Effects of shading treatments on the chlorophyll and carotenoid content in the leaves of YM seedlings
遮荫处理Shading treatment | 叶绿素a Chl a/(mg·g-1) | 叶绿素b Chl b/(mg·g-1) | 总叶绿素Total Chl/(mg·g-1) | 类胡萝卜素Carotenoids/(mg·g-1) |
---|---|---|---|---|
CK | 0.818±0.047b | 0.627±0.034b | 1.433±0.065b | 0.563±0.029b |
30% | 0.957±0.059b | 0.823±0.120a | 1.787±0.237a | 0.712±0.084a |
50% | 1.372±0.120a | 0.835±0.129a | 2.206±0.349a | 0.841±0.149a |
75% | 1.532±0.112a | 0.853±0.061a | 2.315±0.231a | 0.865±0.053a |
95% | 0.728±0.041b | 0.535±0.037b | 1.262±0.071b | 0.496±0.053b |
遮荫处理 Shading treatment | 净光合速率 Pn/ (μmol·m-2·s-1) | 气孔导度 Gs/ (mol·m-2·s-1) | 蒸腾速率Tr/ (mmol·m-2·s-1) | 胞间CO2浓度Ci/ (μmol·mol-1) |
---|---|---|---|---|
CK | 2.041±0.277b | 0.071±0.077b | 117.641±8.039b | 6.496±0.202b |
30% | 2.971±0.093b | 0.100±0.011ab | 125.86±10.850b | 2.558±0.073c |
50% | 4.509±0.245a | 0.090±0.009ab | 340.43±18.821a | 0.853±0.078d |
75% | 5.298±0.806a | 0.158±0.006a | 439.322±8.357a | 0.575±0.053d |
95% | 1.351±0.111b | 0.057±0.017b | 58.547±2.089c | 8.686±0.113a |
表2 不同遮荫处理对云曼红豆杉苗木光合作用相关参数影响
Table 2 Effects of shading treatments on the relevant photosynthetic parameters of YM seedlings
遮荫处理 Shading treatment | 净光合速率 Pn/ (μmol·m-2·s-1) | 气孔导度 Gs/ (mol·m-2·s-1) | 蒸腾速率Tr/ (mmol·m-2·s-1) | 胞间CO2浓度Ci/ (μmol·mol-1) |
---|---|---|---|---|
CK | 2.041±0.277b | 0.071±0.077b | 117.641±8.039b | 6.496±0.202b |
30% | 2.971±0.093b | 0.100±0.011ab | 125.86±10.850b | 2.558±0.073c |
50% | 4.509±0.245a | 0.090±0.009ab | 340.43±18.821a | 0.853±0.078d |
75% | 5.298±0.806a | 0.158±0.006a | 439.322±8.357a | 0.575±0.053d |
95% | 1.351±0.111b | 0.057±0.017b | 58.547±2.089c | 8.686±0.113a |
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