生物技术通报 ›› 2022, Vol. 38 ›› Issue (6): 166-173.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1197
收稿日期:
2021-09-16
出版日期:
2022-06-26
发布日期:
2022-07-11
基金资助:
GAO Xiao-ning1,2(), LIU Rui1, WU Zi-lin1, WU Jia-yun1
Received:
2021-09-16
Published:
2022-06-26
Online:
2022-07-11
摘要:
通过分析宿根矮化病抗感甘蔗品种蔗茎内生真菌和细菌的群落特征,旨在了解内生真菌和细菌类群在寄主抗病中的作用,从而为构建生态防控宿根矮化病提供理论依据。利用Illumina MiSeq高通量测序技术,比较了宿根矮化病感病品种YT60和抗病品种HoCP95988蔗茎内生真菌及细菌的多样性指数、类群组成和物种差异。感病品种YT60蔗茎内生真菌和细菌的Alpha多样性指数高于抗病品种HoCP95988,具有更多的物种组成。蔗茎内生细菌主要分布在变形菌门(Proteobacteria),子囊菌门(Ascomycota)和担子菌门(Basidiomycota)为内生真菌的优势菌门。抗感病甘蔗品种蔗茎内生真菌和细菌种群组成差异明显,其中内生细菌在浮霉菌门(Planctomycetes)、绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadetes)和软壁菌门(Tenericutes)中包含的34个属的物种丰度存在显著差异,并且差异物种在感病品种YT60中呈现较高相对丰度。宿根矮化病抗感甘蔗品种茎部内生真菌和细菌多样性均较为丰富,种群组成存在较大差异,差异物种的分类信息为宿根矮化病生防微生物资源的挖掘提供了线索。
高小宁, 刘睿, 吴自林, 吴嘉云. 宿根矮化病抗感甘蔗品种茎部内生真菌和细菌群落特征分析[J]. 生物技术通报, 2022, 38(6): 166-173.
GAO Xiao-ning, LIU Rui, WU Zi-lin, WU Jia-yun. Characteristics of Endophytic Fungal and Bacterial Community in the Stalks of Sugarcane Cultivars Resistant to Ratoon Stunting Disease[J]. Biotechnology Bulletin, 2022, 38(6): 166-173.
样品名称 Sample name | 高质量序列量 Number of high quality sequence | ASVs | Chao1 | Observed species | Shannon | Simpson | |
---|---|---|---|---|---|---|---|
内生细菌 Endophytic bacteria | YT60 | 552269 | 3061 | 780.91±393.51 | 777.88±392.31 | 4.94±1.96 | 0.82±0.16 |
HoCP95988 | 569775 | 1365 | 360.55±105.85 | 358.20±104.80 | 4.41±0.57 | 0.88±0.05 | |
内生真菌Endophytic fungi | YT60 | 520732 | 176 | 45.59±20.76 | 45.30±20.63 | 4.09±1.07 | 0.87±0.12 |
HoCP95988 | 480771 | 96 | 23.30±15.26 | 22.04±12.52 | 2.62±0.70 | 0.73±0.15 |
表1 不同甘蔗品种蔗茎内生真菌和细菌测序数据、分类单元及多样性指数
Table 1 Sequencing data, ASVs and diversity index of endophytic fungi and bacteria in the stalks of different sugarcane cultivars
样品名称 Sample name | 高质量序列量 Number of high quality sequence | ASVs | Chao1 | Observed species | Shannon | Simpson | |
---|---|---|---|---|---|---|---|
内生细菌 Endophytic bacteria | YT60 | 552269 | 3061 | 780.91±393.51 | 777.88±392.31 | 4.94±1.96 | 0.82±0.16 |
HoCP95988 | 569775 | 1365 | 360.55±105.85 | 358.20±104.80 | 4.41±0.57 | 0.88±0.05 | |
内生真菌Endophytic fungi | YT60 | 520732 | 176 | 45.59±20.76 | 45.30±20.63 | 4.09±1.07 | 0.87±0.12 |
HoCP95988 | 480771 | 96 | 23.30±15.26 | 22.04±12.52 | 2.62±0.70 | 0.73±0.15 |
图2 不同甘蔗品种蔗茎内生细菌(A)和真菌(B)菌群属分类水平结构分布图
Fig. 2 Dominant genera detected from endophytic bacterial(A)and fungal(B)community in the stalks of different sugarcane cultivars
图1 不同甘蔗品种蔗茎内生细菌(A)和真菌(B)菌群门分类水平结构组成
Fig. 1 Dominant phyla detected from endophytic bacterial(A)and fungal(B)community in the stalks of different suga-rcane cultivars
图4 不同甘蔗品种间蔗茎内生细菌LEfSe分析的物种分类学分枝图 蓝色:YT60;红色:HoCP95988
Fig. 4 LEfSe cladogram of endophytic bacteria in the stalks of different sugarcane cultivars Blue:YT60;Red:HoCP95988
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