生物技术通报 ›› 2022, Vol. 38 ›› Issue (8): 206-215.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1377

• 研究报告 • 上一篇    下一篇

不同树龄桑根际土壤原生生物群落组成多样性

王子夜1,2(), 王志刚1,2, 阎爱华1,3()   

  1. 1.河北农业大学林学院,保定071000
    2.河北省林木种质资源和森林保护重点实验室,保定071000
    3.河北省城市森林健康技术创新中心,保定 071000
  • 收稿日期:2021-11-03 出版日期:2022-08-26 发布日期:2022-09-14
  • 作者简介:王子夜,女,硕士研究生,研究方向:林木病理学;E-mail: 952608380@qq.com
  • 基金资助:
    国家重点研发计划(2018YFD0600203-4);河北省重点研发计划项目(19226515D);河北省教育厅优秀青年基金(YQ2014022);现代林业学科群项目(XK1008601519)

Diversity of Soil Protist Community in the Rhizosphere of Morus alba L. at Different Tree Ages

WANG Zi-ye1,2(), WANG Zhi-gang1,2, YAN Ai-hua1,3()   

  1. 1. College of Forestry,Hebei Agricultural University,Baoding 071000
    2. Key Laboratory of Genetic Resources of Forest and Forest Protection of Hebei,Baoding 071000
    3. Hebei Urban Forest Health Technology Innovation Center,Baoding 071000
  • Received:2021-11-03 Published:2022-08-26 Online:2022-09-14

摘要:

原生生物在土壤微生物组中起到枢纽作用,在时间、空间维度上对原生生物分布格局及其对根系微生态影响的研究具有重要意义。本研究按照空间换时间法,选择同一桑园不同树龄(10龄、80龄,200龄左右)桑(Morus alba L.)根际土壤作为样本,利用Illumina高通量测序技术测定土壤原生生物种类多样性、群落组成差异并分析了其驱动因素,为探讨桑根际原生生物的生态学稳定性机制奠定基础。结果表明,不同树龄桑树原生生物群落优势门基本一致,主要为金藻门Chrysophyta、Kinetoplastida、硅藻门Bacillariophyta、轮虫门Rotaliida、卵菌门Oomycetes和Petalomonadida,但相对丰度存在差异,在10a vs 80a、10a vs 200a和80a vs 200a中相对丰度差异显著的属分别为ParacercomonasPetalomonas和变形虫Acanthamoeba、吻滴虫属Rhynchomonas,表明土壤原生生物群落在属水平上组成和相对丰度具有明显树龄变异。原生生物多样性受到土壤理化性质的影响,群落多样性、丰富度和均匀度与氯离子含量极显著负相关,Simpson指数与全盐量显著负相关,群落覆盖度与速效钾和碱解氮含量显著正相关。桑根际原生生物与真菌、细菌之间存在共同的环境偏好或潜在的生物相互作用。

关键词: 桑, 高通量测序, 根际原生生物, 多样性, 群落结构

Abstract:

Protists play a pivotal role in the soil microbiome,and it is important to study the distribution pattern of protists in the temporal and spatial dimensions and their effects on the microecology of the root system. In this study,we followed the trading space for time and selected soil around the rhizosphere of mulberry plants at different ages(10,80 and 200 years old)in the same mulberry(Morus alba L.)orchard as sample. Then we used Illumina high-throughput sequencing technology to determine the diversity of soil protists and differences in community composition and to analyze their drivers,which may provide a basis for exploring the ecological stability mechanisms of mulberry inter-rooted protists. The results showed that the dominant phylum species of mulberry native community were basically the same among different ages,mainly Chrysophyta,Kinetoplastida,Bacillariophyta,Rotaliida,Oomycetes and Petalomonadida,but the relative abundance varied among different ages in Paracercomonas,Petalomonas,Acanthamoeba,and Rhynchomonas were the genera with significant differences in relative abundance at 10a vs 80a,10a vs 200a,and 80a vs 200a years of age,respectively,indicating significant age variation in the composition and relative abundance of the soil native community at the genus level. Native community diversity,abundance and evenness were in very significantly negative correlation with chloride ion,Simpson index was significantly and negatively correlated with total salt and community cover was significantly and positively correlated with potassium ion and alkaline nitrogen content. In sum,there are common environmental preferences or potential biological interactions among the protists and fungi and bacteria in the rhizosphere of mulberry..

Key words: Morus alba L., high throughput sequencing, rhizosphere protist, diversity, community structure